Bony ingrowth induced by treadmill exercise in rats with femoral shaft bone defects

doi:10.3969/j.issn.2095-4344.2014.15.005

Abstract

Abstract:

BACKGROUND: It is well-known that mechanical stimulation could promote fracture healing. However, what kind of mechanical stimulation induced by treadmill exercise can increase the bone conductibility of bone material and promote the healing of bone defect is still unclear.
OBJECTIVE: To evaluate the influence of indirect mechanical stimulation produced by treadmill exercise on bone defect healing and osteogenesis of bone materials.
METHODS: Sprague-Dawley rats at 12 weeks old were used in this study to establish a bone defect of 3 mm in diameter and height at the left distal femur. Afterwards, calcium sulphate scaffolds were implanted into the defects. The rats were divided into treadmill exercise group and control group. Treadmill exercise was began at 1 week postoperatively, 10 m/min, 45 minutes per day, 5 days per week, for 3 weeks. Control group did not receive any exercise. Micro-computed tomography was used to determine bone formation in the bone defects at 1, 2, 3, and 4 weeks after surgery. The sections of left distal femur were subject to hematoxylin-eosin staining, the new bone formation and degradation of bone materials in the bone defects were observed.
RESULTS AND CONCLUSION: Micro-CT analysis showed that, a small amount of new bone formed in both treadmill exercise group and control group at 1 week after surgery. In treadmill exercise group, new bone formation was significantly higher than the control group at 2, 3, 4 weeks (P < 0.05). At 4 weeks, histological
results also confirmed the difference of new bone formation in bone defect between treadmill exercise group and control group. In addition, bone mineral density of treadmill exercise group was higher than that of control group at 2, 3, 4 weeks, but no significant difference was found (P > 0.05). The results suggest that moderate treadmill exercise could promote bone defect healing and enhance osteoconductivity of bone substitute.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: motor activity, calcium sulfate, osteogenesis, tomography, X-ray

CLC Number:

R318

Zhang Jie, Zhang Wen, Chen Xi, Luo Zong-ping, Yang Hui-lin. Bony ingrowth induced by treadmill exercise in rats with femoral shaft bone defects[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(15): 2320-2325.

Figures/Tables 2

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