Prostaglandin E1 effects on proliferation of rat bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2014.14.005

Abstract

Abstract:

BACKGROUND: Currently, there are few studies about prostaglandin E1 on the proliferation of bone marrow mesenchymal stem cells.
OBJECTIVE: To explore the effects of prostaglandin E1 on the proliferation of bone marrow mesenchymal stem cells.
METHODS: Bone marrow mesenchymal stem cells isolated from Sprague Dawley rats were cultured in vitro. Passage 3 cells were selected to identify cell surface phenotypes by flow cytometry. Cells were seeded onto 96-well microtiter plates at a density of 1×104 cells/well, and then treated with prostaglandin E1 at concentrations of 0, 1, 2, 5, 10, 20, 40, 100 μg/L for 3 days. Cell counting kit-8 assay was applied to assess cellular proliferation, and detect the optimal proliferative concentration of prostaglandin E1 (10%) effects on the proliferation of bone marrow mesenchymal stem at 24, 48 and 72 hours.
RESULTS AND CONCLUSION: The positive rates of passage 3 cells were 4.93% for CD11b/c, 99.93% for CD29, 0.1% for CD45, 99.58% for CD90. In vitro proliferation of bone marrow mesenchymal stem cells could be promoted by prostaglandin E1 at concentrations of 1, 2, 5, 10, 20, 40 and 100 μg/L, and the optimal concentration was 10 μg/L. The proliferation of bone marrow mesenchymal stem cells was promoted dramatically after intervention with 10 μg/L prostaglandin E1 for 48 hours (P < 0.05), and peaked at 72 hours in a time-dependent manner.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, mesenchymal stem cells, prostaglandins

CLC Number:

R394.2

Zeng Kuan, Jiang Hui-qi, Wang Meng, Hua Ping, Zhang Hong-wu, Deng Bao-ping, Zhang Shu-ming, Yang Yan-qi. Prostaglandin E1 effects on proliferation of rat bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(14): 2161-2166.

Figures/Tables 5

References

[1] Minguell JJ, Erices A. Mesenchymal stem cells and the treatment of cardiac disease. Exp Biol Med (Maywood).2006; 231(1):39-49.
[2] Devine SM. Mesenchymal stem cells: will they have a role in the clinic?. J Cell Biochem Suppl.2002,38:73-79.
[3] Pittenger MF, Mackay AM, Beck SC, et al. Multilineage potential of adult human mesenchymal stem cells. Science. 1999;284(5411):143-147.
[4] Menasche P. Current status and future prospects for cell transplantation to prevent congestive heart failure.Semin Thorac Cardiovasc Surg.2008;20(2):131-137.
[5] Lazarus HM, Koc ON, Devine SM, et al.Cotransplantation of HLA-identical sibling culture-expanded mesenchymal stem cells and hematopoietic stem cells in hematologic malignancy patients. Biol Blood Marrow Transplant.2005; 11(5):389-398.
[6] Tambara K, Sakakibara Y, Sakaguchi G, et al. Transplanted skeletal myoblasts can fully replace the infarcted myocardium when they survive in the host in large numbers. Circulation. 2003;108(Suppl 1):I259-I263.
[7] Gao Y, Xu P,Chen L,et al.Prostaglandin E1 encapsulated into lipid nanoparticles improves its anti-inflammatory effect with low side-effect.Int J Pharm. 2010;387(1-2):263-271.
[8] Wu CC, Wu CI, Wang WY, et al.Low concentrations of resveratrol potentiate the antiplatelet effect of prostaglandins. Planta Med.2007;73(5):439-443.
[9] Zhao XS, Pan W, Bekeredjian R, et al. Endogenous endothelin-1 is required for cardiomyocyte survival in vivo. Circulation.2006;114(8):830-837.
[10] 杨焕纳,崔正军,Han Seung-Kyu,等.前列腺素E1对人类脂肪源性基质细胞体外增殖的影响[J].中国组织工程研究与临床康复, 2011,15(14):2517-2520.
[11] Grassel S, Anders S.Cell-based therapy options for osteochondral defects. Autologous mesenchymal stem cells compared to autologous chondrocytes. Orthopade. 2012; 41(5):415-428, 429-430.
[12] Xu N, Liu H, Qu F, et al. Hypoxia inhibits the differentiation of mesenchymal stem cells into osteoblasts by activation of Notch signaling. Exp Mol Pathol. 2013;94(1):33-39.
[13] Fink T, Zachar V. Adipogenic differentiation of human mesenchymal stem cells. Methods Mol Biol.2011;698: 243-251.
[14] Law S,Chaudhuri S.Mesenchymal stem cell and regenerative medicine: regeneration versus immunomodulatory challenges. Am J Stem Cells.2013;2(1):22-38.
[15] Cai B, Li J, Wang J, et al.microRNA-124 regulates cardiomyocyte differentiation of bone marrow-derived mesenchymal stem cells via targeting STAT3 signaling. Stem Cells.2012;30(8):1746-1755.
[16] Zhang Y, Chu Y, Shen W, et al.Effect of 5-azacytidine induction duration on differentiation of human first-trimester fetal mesenchymal stem cells towards cardiomyocyte-like cells. Interact Cardiovasc Thorac Surg.2009;9(6):943-946.
[17] Soma T, Kishimoto J, Fisher D.Isolation of mesenchymal stem cells from human dermis. Methods Mol Biol.2013; 989:265-274.
[18] Lubis A M, Sandhow L, Lubis VK, et al. Isolation and cultivation of mesenchymal stem cells from iliac crest bone marrow for further cartilage defect management. Acta Med Indones, 2011,43(3):178-184.
[19] Conget PA, Minguell JJ. Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells. J Cell Physiol.1999;181(1):67-73.
[20] Gao X, Zhang J, Zhang J, et al.Identification of Rat Respiratory Mucosa Stem cells and comparison of the early neural differentiation potential with the bone marrow mesenchymal stem cells in vitro. Cell Mol Neurobiol. 2014; 34(2):257-268.
[21] 李晓峰,赵劲民,苏伟,等.大鼠骨髓间充质干细胞的培养与鉴定[J]. 中国组织工程研究与临床康复, 2011,15(10):1721-1725.
[22] 杨宇辉,吴继雄.大鼠骨髓间充质干细胞和内皮祖细胞的分离培养及鉴定[J].中国组织工程研究与临床康复, 2009,13(40):7876- 7880.
[23] Liu Y, Wang L, Fatahi R, et al.Isolation of murine bone marrow derived mesenchymal stem cells using Twist2 Cre transgenic mice. Bone.2010;47(5):916-925.
[24] Kitazawa Y, Li X K, Xie L, et al.Bone marrow-derived conventional, but not cloned, mesenchymal stem cells suppress lymphocyte proliferation and prevent graft- versus-host disease in rats. Cell Transplant.2012; 21(2-3): 581-590.
[25] Feng J, Wu G, Liu R, et al. Prostaglandin E1 (PGE1) reduces cardiac-derived TXA2 release in ischaemic arrest in isolated working rat heart. Int J Cardiol. 1996;55(3):265-270.
[26] Bai W, Zheng X, Zhou L, et al. Prostaglandin E1 dose-dependently promotes stability of atherosclerotic plaque in a rabbit model. Can J Physiol Pharmacol. 2012;90(2): 131-139.
[27] Li J, Yang P, Li A, et al. Cardioprotective effect of liposomal prostaglandin E1 on a porcine model of myocardial infarction reperfusion no-reflow. Journal of Zhejiang University Science B.2011;12(8):638-643.
[28] Jia Y,Lin J,Zeng W,et al.Effect of prostaglandin on luteinizing hormone-stimulated proliferation of theca externa cells from chicken prehierarchical follicles. Prostaglandins Other Lipid Mediat.2010;92(1-4):77-84.
[29] 符晓阳,蔡传奇,郑鸿,等.前列腺素E1促进血管内皮细胞增生和迁移及其机制[J].中华医学杂志, 2013,93(3):222-225.