Biological characterization of C57 mouse bone marrow mesenchymal stem cells using a whole bone marrow adherent culture technique

doi:10.3969/j.issn.2095-4344.2014.01.008

Abstract

Abstract:

BACKGROUND: Under mitomycin C treatment, feeder cells appear to have restricted proliferation, but they are still able to secret different cytokines. Non-mesenchymal stem cells from the bone marrow and secreted factors in plasma maintain the micro-environment suitable for the growth of mesenchymal stem cells that can improve the yield of mesenchymal stem cells.
OBJECTIVE: To study the biological characteristics of C57 mouse bone marrow mesenchymal stem cells isolated using a whole bone marrow adherent culture technique.
METHODS: Using the whole bone marrow adherent culture technique, purified and amplified C57 mouse bone marrow mesenchymal stem cells were harvested. Cell proliferation kinetics, immune cell surface markers, multiple differentiation potential and cell cycle were detected.
RESULTS AND CONCLUSION: Using the whole bone marrow culture, mouse bone marrow mesenchymal stem cells were harvested and capable of adhering to the plastic culture vessel. The obtained cells expressed CD45, CD105 and Sca-1, but were negative for CD34, CD33 and C-kit. The doubling time was (57.11±1.5) hours. The cells could be induced to differentiate into osteoblasts, adipocytes and chondrocytes. The cell cycle analysis showed that 64% of cells were in G0-G1 phase. These indicates that C57 mouse bone marrow mesenchymal stem cells isolated using a whole bone marrow adherent culture technique have biological characteristics of mesenchymal stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, mesenchymal stem cells, cell culture techniques, induced pluripotent stem cells

CLC Number:

R394.2

Zhang Rong-yao, Bi Xiao-juan, Ma Yan, Duan Xian-lin, Jiang Ming . Biological characterization of C57 mouse bone marrow mesenchymal stem cells using a whole bone marrow adherent culture technique[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(1): 45-50.

Figures/Tables 3

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