Cytocompatibility of calcium metaphosphate nanoparticles

doi:10.3969/j.issn.2095-4344.2013.38.015

Abstract

Abstract:

BACKGROUND: Calcium metaphosphate has excellent biocompatibility, degradability, and cell affinity. Human bone marrow mesenchymal stem cells can grow and proliferate in the pores of the porous calcium metaphosphate, but less is known about calcium metaphosphate nanoparticles.
OBJECTIVE: To prepare calcium metaphosphate nanoparticles, and to analyze the effect of calcium metaphosphate nanoparticles at different concentrations on apoptosis of human bone marrow mesenchymal stem cells by flow cytometry.
METHODS: The calcium metaphosphate nanoparticles were prepared by wet ball milling. Scanning electron microscopy and transmission electron microscopy were used to observe the morphology of the calcium metaphosphate nanoparticles, and the crystal structure of nanoparticles was analyzed by X-ray diffraction. Calcium metaphosphate nanoparticles were mixed in the CYAGON OricellTM basal medium, and the ncentrations of calcium metaphosphate nanoparticles in the medium were 10, 1, 0.1 mg/L. Human bone marrow mesenchymal stem cells were cultured for 7 days in the above-mentioned media, and apoptosis of human bone marrow mesenchymal stem cells was analyzed by flow cytometry.
RESULTS AND CONCLUSION: Calcium metaphosphate nanoparticles were successfully prepared by wet ball milling, irregular in shape, and the mean diameter was 10-30 nm. X-ray diffraction results showed the crystal structure of nonaparticles was mainly β-Ca(PO3)2. The cell ratio of G0/G1 phase and G2/M phase in 10 mg/L group was obviously higher than that in 1, 0.1 mg/L groups (P < 0.01). The cell apoptosis rates during the early, middle, late stages in 10 mg/L group were obviously higher than those in 1, 0.1 mg/L groups (P < 0.01), and the total cell apoptosis was also significantly increased in 10 mg/L group (P < 0.01). These findings indicate that human bone marrow mesenchymal stem cells proliferation can be inhibited by calcium metaphosphate nanoparticles, and apoptosis rate is increased significantly when the concentration of calcium metaphosphate nanoparticles increases from 1 mg/L to 10 mg/L.

Key words: biocompatible materials, nanoparticles, calcium phosphates, histocompatibility

CLC Number:

R318

Wu Yue-heng1, Mai Li-ping1, Chen Peng2, Zhang Ling-min, Huang Huan-lei, Zeng Xiang-jun,Tang Shun-qing, Xiao Xue-jun, Yu Xi-yong. Cytocompatibility of calcium metaphosphate nanoparticles[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.38.015.

Figures/Tables 6

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