Chinese Journal of Tissue Engineering Research
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Wu Guang-xin, Wang Jian-guo, Du Xiao-yan
Received:
2012-12-18
Revised:
2013-01-19
Online:
2013-09-17
Published:
2013-09-17
Contact:
Du Xiao-yan, Master, Chief physician, Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Jiamusi University, Jiamusi 154000, Heilongjiang Province, China
dxy00007@163.com
About author:
Wu Guang-xin★, Studying for master’s degree, Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Jiamusi University, Jiamusi 154000, Heilongjiang Province, China
wgx629582@163.com
CLC Number:
Wu Guang-xin, Wang Jian-guo, Du Xiao-yan. Bone marrow mononuclear cells combined with nano-hydroxyapatite/collagen for repair of mandibular defects[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.38.014.
术后4周时,实验组见缺损区材料少量吸收,与周围骨组织融合尚可,质地略硬;对照组材料未见明显吸收,纤维组织充填,与周围边界明显,质地软;空白组见肉芽组织长入,炎性渗出明显。术后8周时,实验组材料小部分吸收,骨痂形成明显,质地较硬;对照组材料少量吸收,形成少量骨痂,残存材料较多,质地较软,硬度不及实验组;空白组肉芽组织增生明显。术后12周时实验组骨缺损区表面成骨良好,骨膜包被,质地硬;对照组骨痂形成明显,仍有材料残存,硬度不及实验组;空白组肉芽组织增生明显,有少量骨形成,见图1。 2.3 各组影像学分析结果 术后4周时,实验组见骨缺损区网状高密度影出现,材料少量吸收,有早期成骨现象,与周围组织界限清;对照组骨缺损成低密度影,有材料阻射影,与周围界限清,无明显成骨;空白组明显低密度影。术后8周时,实验组材料小部分吸收,出现絮状高密度影,成骨较多,密度略高;对照组材料少量吸收,少量成骨,密度较低,与周围组织界限尚清;空白组明显低密度影,术后12周时,实验组材料明显吸收,与周围组织界限模糊,密度明显增高,但较正常骨略低;对照组密度较实验组低,可见低密度影;空白组仍见明显低密度影,见图2。"
2.6 各组骨缺损区CT值测定比较分析结果 术后4,8,12周牙CT扫描骨缺损区内6个不同位点,记录CT值并取均值后进行统计分析:实验组成骨CT值高于对照组和空白组(P < 0.05),说明实验组在成骨方面优于对照组及空白组,见表1。 2.7 各组骨缺损区新生骨占骨缺损面积的百分比比较分析结果 将术后4,8,12周3个时间段所有组织连续切片,进行系统选片,每块组织选5张,光镜100倍下每张切片系统随机选取8个视野,在图像分析仪下进行组织学形态分析,测定术后各个时间点上新生骨小梁面积所占修复区面积的比值。发现实验组新生骨小梁面积占修复区面积的比值高于对照租及空白组(P < 0.05),证明实验组成骨效果优于对照组及空白组,见表2。"
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