Chinese Journal of Tissue Engineering Research

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Bone marrow mononuclear cells combined with nano-hydroxyapatite/collagen for repair of mandibular defects

Wu Guang-xin, Wang Jian-guo, Du Xiao-yan   

  1. Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Jiamusi University, Jiamusi  154000, Heilongjiang Province, China
  • Received:2012-12-18 Revised:2013-01-19 Online:2013-09-17 Published:2013-09-17
  • Contact: Du Xiao-yan, Master, Chief physician, Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Jiamusi University, Jiamusi 154000, Heilongjiang Province, China dxy00007@163.com
  • About author:Wu Guang-xin★, Studying for master’s degree, Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Jiamusi University, Jiamusi 154000, Heilongjiang Province, China wgx629582@163.com

Abstract:

BACKGROUND: Nano-hydroxyapatite/collagen combined with mononuclear cells can promote the growth of a variety of stem cells to induce the formation of new bone and vascularization, finally inducing osteogenesis.
OBJECTIVE: To investigate the feasibility of bone marrow mononuclear cells combined with the nano-hydroxyapatite/collagen for repair of mandibular defects in a rabbit.
METHODS: Twenty-seven New Zealand white rabbits were selected to prepare bilateral mandibular bone defect models, and then divided into three groups. In experiment group, bone marrow mononuclear cells combined with the nano-hydroxyapatite/collagen were implanted into mandibular defects; in control group, nano-hydroxyapatite/collagen scaffold was implanted; and in blank control group, nothing was implanted. Tissue specimens were prepared at weeks 4, 8, 12 for gross observation, imaging analysis, hematoxylin-eosin staining and scanning electron microscope.
RESULTS AND CONCLUSION: The imaging examination and histological staining showed that the bone quality and healing degree in the experimental group was better than those in the other groups. Scanning electron microscope showed that better histocompatibility and no inflammation reaction in the experimental group. Dental CT data showed that the experimental group had better repair effect than the other groups (P < 0.05). These findings indicate that bone marrow mononuclear cells combined with the nano-hydroxyapatite/collagen have capacity of bone induction and bone formation, which can be used to repair mandibular defects.

Key words: biocompatible materials, nanoparticles, collagen, stents

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