Chinese Journal of Tissue Engineering Research

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Cytotoxicity tests for the mussel adhesive protein dressing for wound healing

Liu Qian1, 2, Lan Hua-lin1, 3, Gu Ming4, Gao Min4, Wang Zhao-xu1   

  1. 1Laboratory of Biomaterials and Tissue Engineering, National Institutes for Food and Drug Control, Beijing  100050, China; 2Center of Biological Safety Evaluation, Jiangsu Province Medical Instrument Testing Institute, Nanjing  210022, Jiangsu Province, China; 3Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering of Chongqing University, Chongqing  400044, China; 4National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Science, Beijing  100190, China
  • Received:2013-07-04 Revised:2013-07-12 Online:2013-09-17 Published:2013-09-17
  • Contact: Wang Zhao-xu, Associate investigator, Laboratory of Biomaterials and Tissue Engineering, National Institutes for Food and Drug Control, Beijing 100050, China WangZX@nicpbp.org.cn
  • About author:Liu Qian★, Master, Laboratory of Biomaterials and Tissue Engineering, National Institutes for Food and Drug Control, Beijing 100050, China; Center of Biological Safety Evaluation, Jiangsu Province Medical Instrument Testing Institute, Nanjing 210022, Jiangsu Province, China liuqian0916@126.com
  • Supported by:

    the Projects of the Ministry of Science and Technology of China, No. 2012BAI18B02

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Abstract:

BACKGROUND: In the cytotoxicity test of the mussel adhesive protein dressing for wound healing, because of positive charge properties of the protein, when extracting ratio is 1:9, the cells exhibit poly-group phenomenon that results in errors in the cytotoxicity test of mussel adhesive protein samples.
OBJECTIVE: According to the existing standards, to improve the leaching proportion and pretreatment of mussel adhesive protein dressing for wound healing based on the special properties and working condition of mussel adhesive protein.
METHODS: (1) Extract method: Extract solution of mussel adhesive protein dressing was prepared with mussel adhesive protein dressing and cell medium at extracting ratios of 1:9 and 1:131. Then, L929 cells were cultured in extract solutions of mussel adhesive protein dressing, natural latex and high-density polyethylene, respectively. (2) Method of direct contact: Distilled water, solutions of mussel adhesive protein dressing, dimethyl sulfoxide and cell medium were used to culture L929 cells.
RESULTS AND CONCLUSION: When the extracting ratio was 1:9, the cells agglomerated which is not suitable for cytotoxicity test. When the extracting ratio was 1:131, flocculated sediment and cell aggregation disappeared, the cytotoxicity test results showed no cytotoxicity with higher reliability. Direct contact method showed the samples had no cytotoxicity. The extract method with adjusted extracting radio or direct contact method can be applied to test the in vitro cytotoxicity of mussel adhesive protein dressing for wound healing.

Key words: mucins, biocompatible materials, biological dressings, cytotoxicity, immunologic

CLC Number: