Effects of perfluorotributylamine/alginate/bioglass biomaterials on viability and osteogenic differentiation of adipose-derived stem cells

doi:10.3969/j.issn.2095-4344.1688

Abstract

Abstract:

BACKGROUND: Under hypoxia, the in vivo application of bone tissue engineering materials is limited. To solve the problem of hypoxia, an oxygen-carrying bone tissue engineering material is required.
OBJECTIVE: To prepare an alginate/bioactive glass scaffold with optimal perfluorotributylamine concentration and to explore the effect of the composite scaffold on in vitro proliferation and osteogenic differentiation of adipose-derived stem cells under hypoxia.
METHODS: Alginate/bioactive glass biomaterials containing 0% (control), 5%, 10%, and 20% perfluorotributylamine were prepared to measure oxygen-releasing curves and cytotoxicity. Under the condition of hypoxia, the rabbit adipose-derived stem cells were implanted into in alginate/bioactive glass materials containing different concentrations of perfluorotributylamine. The cellular proliferation in the four groups was compared, and the optimal perfluorotributylamine concentration in the scaffolds was selected. The adipose-derived stem cells were implanted onto the optimal scaffold, and cell adhesion, alkaline phosphatase activity and osteogenesis-related genes were detected under normoxia and hypoxia.
RESULTS AND CONCLUSION: (1) Under hypoxic conditions, the cell proliferation in the 10% perfluorotributylamine group was significantly higher than that in the 5% perfluorotributylamine group and the control group (P < 0.05). (2) Under hypoxic conditions, the cells in the 10% perfluorotributylamine group adhered well, and the mean absorbance value of adherence spots was significantly higher than that in the control group (P < 0.05). (3) After 7 days of osteogenic induction under hypoxia, the activity of alkaline phosphatase and the expression of osteogenesis-related genes in the 10% perfluorotributylamine group were significantly higher than those in the control group (P < 0.05). To conclude, the alginate/bioactive glass bone tissue-engineered scaffold with 10% perfluorotributylamine can promote the proliferation, adhesion and osteogenic differentiation of rabbit adipose-derived stem cells under hypoxia.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Biocompatible Materials, Anoxia, Adipose Tissue, Stem Cells, Cell Proliferation, Cell Adhesion, Tissue Engineering

CLC Number:

Luo Kai, Yang Yafeng, Ma Teng, Xia Bing, Huang Liangliang, Huang Jinghui, Luo Zhuojing. Effects of perfluorotributylamine/alginate/bioglass biomaterials on viability and osteogenic differentiation of adipose-derived stem cells[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(13): 1995-2001.

Figures/Tables 2

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