Construction of a tissue-engineered urethral scaffold using adipose-derived stem cells combined with biodegradable materials in vitro

doi:10.3969/j.issn.2095-4344.1677

Abstract

Abstract:

BACKGROUND: Studies have shown that adipose-derived stem cells have the ability to differentiate into urothelial cells as seed cells for urethral tissue engineering reconstruction. To this end, we developed a novel tissue-engineered urethral scaffold carrying adipose-derived stem cells.
OBJECTIVE: To test the feasibility of using human adipose-derived stem cells and collagen materials to construct a new type of tissue-engineered urethral scaffold.
METHODS: Human adipose-derived stem cells were isolated from the waste tissue of liposuction. The isolated cells were then cultured and amplified. Collagen and polylactic acid were used to construct urethral scaffolds by solvent volatilization and electrospinning method. The scaffolds were divided into two groups according to whether collagen was added or not. The cultured adipose-derived stem cells were seeded onto the constructed urethral scaffolds and cultured for 7 days. MTT analysis, live/dead staining and hematoxylin-eosin staining were done to evaluate the cell growth on the novel tissue-engineered urethral scaffold thereafter.
RESULTS AND CONCLUSION: (1) At 7 days after transplantation, the cells in the collagen-containing group grew well, there were two or three layers of cells on the scaffold surface, and some cells infiltrated and grew inside the scaffold. In the collagen-free group, the cells distributed in a low density, and only one or two layers of cells formed, mainly on the surface of scaffolds. (2) More than 90% of the implanted cells in the collagen-containing group were living cells, suggesting that the cells survived well and scaffolds had good biocompatibility. In the collagen-free group, the cells on the scaffolds were in poor condition, with only about 50% living cells. (3) The cells on the scaffolds containing collagen showed a trend of continuous proliferation, and the collagen-containing scaffold was more suitable for the growth and proliferation of cells as compared with the collagen-free scaffold. Overall, it is feasible to construct a new type of tissue-engineered urethral scaffold using human adipose-derived stem cells and polylactic acid/collagen, providing a new approach for the urethral repair and reconstruction.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Urethral Diseases, Adipose Tissue, Stem Cells, Collagen, Tissue Engineering

CLC Number:

Shi Jianguo, Wang Weining, Li Chunwu, Bai Xinyu, Chen Yudong. Construction of a tissue-engineered urethral scaffold using adipose-derived stem cells combined with biodegradable materials in vitro[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(13): 1989-1994.

Figures/Tables 3

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