Chinese Journal of Tissue Engineering Research ›› 2024, Vol. 28 ›› Issue (15): 2430-2436.doi: 10.12307/2024.408

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Preparation, characterization, and application of acylated collagen with anhydride

Li Weizhao, Zhou Hui, Peng Xinsheng, Li Baohong   

  1. School of Pharmacy, Guangdong Medical University, Dongguan 523808, Guangdong Province, China
  • Received:2023-06-05 Accepted:2023-07-17 Online:2024-05-28 Published:2023-09-23
  • Contact: Li Baohong, PhD, Professor, School of Pharmacy, Guangdong Medical University, Dongguan 523808, Guangdong Province, China Peng Xinsheng, PhD, Professor, School of Pharmacy, Guangdong Medical University, Dongguan 523808, Guangdong Province, China
  • About author:Li Weizhao, Master candidate, School of Pharmacy, Guangdong Medical University, Dongguan 523808, Guangdong Province, China
  • Supported by:
    Key Research Project for Ordinary Universities of Guangdong Provincial Department of Education, No. 2021ZDZX2039 (to PXS); Chongbu Qiang Key Project of Guangdong Medical University, No. 4SG21277P (to PXS)

Abstract: BACKGROUND: Collagen is a biomedical material with good biocompatibility, low toxicity, low immunoactivity, and high cellular affinity. However, the defects such as hydrophilicity and poor thermal stability are the key technical problems that need to be solved urgently in biomedical, tissue engineering, and other applications. 
OBJECTIVE: To elaborate on the preparation method, characterization, and application progress of acylated collagen.
METHODS: PubMed, X-mol, and CNKI databases were used to search the literature on acylated collagen preparation methods, characterization, and application. The search time was from January 1992 to May 2023. The English search terms were “acylated collagen, modified collagen, water-soluble collagen, acetic anhydride”. Chinese search terms were “acylated collagen, modified collagen, water-soluble collagen”. After an initial screening of all articles according to inclusion and exclusion criteria, 53 articles with high relevance were retained for review.
RESULTS AND CONCLUSION: (1) The preparation of acylated collagen is mainly obtained by the acylation reaction of acid anhydride, and the reaction sites are mainly ε-amino and N-terminus-amino of collagen, and the reaction environment of acylation reaction needs to be carried out in an alkaline environment. (2) Succinic anhydride is the preferred reactant of acylated collagen. Carbonic anhydride, citric anhydride, and bifunctional modifiers with acylation ability have been excavated in recent years to meet various requirements, but it is still limited to laboratory preparation; complex preparation process is difficult to industrialize, and follow-up research is needed to continuously improve. (3) The characterization methods of acylated collagen are similar to those of collagen, but there is still a lack of standards for the water-soluble characterization of acylated collagen. (4) In recent years, acylated collagen has been gradually used in the preparation of lenses, hydrogels, and dressings, as well as cosmetic raw materials. However, there is still little research on the application of acylated collagen, and there is a lack of complete in vivo experiments to verify the practicality of acylated collagen.

Key words: collagen, acylation, anhydride, acylated collagen, characterization, application area, review

CLC Number: