Chinese Journal of Tissue Engineering Research ›› 2024, Vol. 28 ›› Issue (1): 68-73.doi: 10.12307/2023.915

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Effect of overexpression of protein phosphatase 2Cm on transcriptome of human renal tubular epithelial cells

Zhang Li1, 2, 3, 4, 5, Yang Wenjun1, 2, 3, 4, 5, Sang Xiaohong1, 2, 3, 4, 5, Han Yuanyuan1, 2, 3, 4, 5, Mao Zhijie1, 2, 3, 4, 5, Wang Shun1, 2, 3, 4, 5 , Lu Chen1, 2, 3, 4, 5   

  1. 1Nephrology Center of First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China; 2Xinjiang Clinical Research Center of Renal Replacement Therapy, Urumqi 830011, Xinjiang Uygur Autonomous Region, China; 3Xinjiang Branch of National Clinical Research Center for Kidney Disease, Urumqi 830011, Xinjiang Uygur Autonomous Region, China; 4Xinjiang Blood Purification Medical Quality Control Center, Urumqi 830011, Xinjiang Uygur Autonomous Region, China; 5Institute of Nephrology of Xinjiang, Urumqi 830011, Xinjiang Uygur Autonomous Region, China
  • Received:2022-11-29 Accepted:2023-01-29 Online:2024-01-08 Published:2023-06-28
  • Contact: Lu Chen, MD, Chief physician, Nephrology Center of First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China; Xinjiang Clinical Research Center of Renal Replacement Therapy, Urumqi 830011, Xinjiang Uygur Autonomous Region, China; Xinjiang Branch of National Clinical Research Center for Kidney Disease, Urumqi 830011, Xinjiang Uygur Autonomous Region, China; Xinjiang Blood Purification Medical Quality Control Center, Urumqi 830011, Xinjiang Uygur Autonomous Region, China; Institute of Nephrology of Xinjiang, Urumqi 830011, Xinjiang Uygur Autonomous Region, China
  • About author:Zhang Li, MD, Chief physician, Nephrology Center of First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China; Xinjiang Clinical Research Center of Renal Replacement Therapy, Urumqi 830011, Xinjiang Uygur Autonomous Region, China; Xinjiang Branch of National Clinical Research Center for Kidney Disease, Urumqi 830011, Xinjiang Uygur Autonomous Region, China; Xinjiang Blood Purification Medical Quality Control Center, Urumqi 830011, Xinjiang Uygur Autonomous Region, China; Institute of Nephrology of Xinjiang, Urumqi 830011, Xinjiang Uygur Autonomous Region, China
  • Supported by:
    the National Natural Science Foundation of China, No. 81960145 (to ZL)

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Abstract: BACKGROUND: A previous study by our group found that protein phosphatase 2Cm (PP2Cm) null mice developed significantly fewer symptoms of renal failure relative to wild-type mice, and thus it was speculated that PP2Cm may play an important protective role in the development of renal fibrosis, however, the molecular mechanisms remain undefined.
OBJECTIVE: To investigate the effect of the PP2Cm gene on the transcriptome of human renal tubular epithelial cells.
METHODS: Cultured human renal tubular epithelial cells were transfected with the PP2Cm gene into human renal tubular epithelial cells using plasmids. The expression of PP2Cm in the cells was detected by fluorescence quantitative PCR assay and western blot assay, and subsequently, cell RNA was separately extracted for transcriptome sequencing to look for differentially expressed genes between transfected and control groups. The resulting differential genes were further subjected to GO analysis and KEGG analysis using bioinformatics methods.
RESULTS AND CONCLUSION: There were 796 differentially expressed genes, 553 of which were downregulated genes and 243 upregulated genes, in human renal tubular epithelial cells transfected with the PP2Cm gene compared with untransfected blank cells by sequencing analysis. GO analysis results showed that the upregulated genes were significantly enriched in cellular biosynthetic processes, protein translation, intrinsic apoptotic signaling pathways, and so on. The downregulated expressed genes were significantly enriched in endothelial cell proliferation, cell adhesion and other signaling pathways. KEGG analysis results showed that the significantly up-regulated genes were enriched in metabolism-related signaling pathways such as amino acid metabolism and biosynthesis. The downregulated expressed genes were significantly enriched in signaling pathways such as pantothenate and coenzyme A biosynthesis. Our results show that PP2Cm overexpression can affect a number of signaling pathways related to a range of biological processes in renal tubular epithelial cells, which may be important in metabolism-related signaling pathways such as amino acid metabolism and biosynthesis. 

Key words: renal tubular epithelial cell, PP2Cm, transcriptome sequencing, enrichment analysis, signaling pathway

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