Regulatory effects of cAMP response element-binding protein on hippocampal brain-derived neurotrophic factor level in a rat model of drug-resistant epilepsy

doi:10.12307/2023.847

Abstract

Abstract: BACKGROUND: The formation mechanism of drug-resistant epilepsy is still unclear, and the role of cAMP response element-binding protein (CREB) and (brain-derived neurotrophic factor (BDNF) in the formation of drug-resistant epilepsy has been widely studied.
OBJECTIVE: To regulate the expression of CREB in the hippocampus of normal rats by lentivirus transfection and to explore its effect on the formation of drug-resistant epilepsy and the expression of BDNF in the hippocampus of rats.
METHODS: Lentivirus overexpression or interfering was used to regulate the expression level of CREB in the hippocampus of rats and detect the virus transfection results. Lithium-pilocarpine induced epilepsy models were established in rats 7 days after lentivirus injection. Sodium phenytoin and phenobarbital were then used for resistance selection. Drug-resistant epileptic rats were screened and divided into overexpression group, overexpression control group, interference group and interference control group. The frequency of epileptic seizures during the screening process was observed and the expression levels of CREB and BDNF in the hippocampus of rats were measured by western blot and real-time fluorescence quantitative PCR.
RESULTS AND CONCLUSION: Fourteen days after virus transfection, the hippocampus of rats was covered with green fluorescence, indicating that the virus transfection level was good. The frequency of epileptic seizures in the overexpression group was higher than that in the overexpression control group and the frequency of epileptic seizures in the interference group was lower than that in the interference control group. The expression levels of CREB and BDNF in the overexpression group were higher than those in the overexpression control group, while the expression levels of CREB and BDNF in the interference group were lower than those in the interference control group. These results indicate that the regulation of CREB expression level in the hippocampus of rats can affect epileptic seizures and formation of drug-resistant epilepsy in rats, and regulating CREB expression may affect the expression level of BDNF in the hippocampus of rats with drug-resistant temporal lobe epilepsy.

Key words: drug-resistant epilepsy, brain-derived neurotrophic factor, cAMP response element-binding protein, CREB-BDNF-TrkB signaling pathway

CLC Number:

Li Jingxuan, Shi Dai, Wu Guofeng. Regulatory effects of cAMP response element-binding protein on hippocampal brain-derived neurotrophic factor level in a rat model of drug-resistant epilepsy[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(35): 5659-5664.

Figures/Tables 6

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