Chinese Journal of Tissue Engineering Research ›› 2023, Vol. 27 ›› Issue (35): 5659-5664.doi: 10.12307/2023.847
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Li Jingxuan1, Shi Dai1, Wu Guofeng2
Received:
2022-10-20
Accepted:
2022-11-30
Online:
2023-12-18
Published:
2023-06-02
Contact:
Wu Guofeng, MD, Professor, Chief physician, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China
About author:
Li Jingxuan, Master candidate, Guizhou Medical University, Guiyang 550004, Guizhou Province, China
Supported by:
CLC Number:
Li Jingxuan, Shi Dai, Wu Guofeng. Regulatory effects of cAMP response element-binding protein on hippocampal brain-derived neurotrophic factor level in a rat model of drug-resistant epilepsy[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(35): 5659-5664.
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2.2 耐药性癫痫模型建立 注射过表达慢病毒的大鼠共有10只成为慢性癫痫模型,最终筛选出 5只耐药性癫痫大鼠,5只药物敏感性癫痫大鼠;注射过表达对照慢病毒的大鼠共9只成为慢性癫痫模型,最终筛选出3只耐药性癫痫大鼠,6只药物敏感性癫痫大鼠;注射干扰慢病毒的大鼠共14只成为慢性癫痫模型,最终筛选出3只耐药性癫痫大鼠,11只药物敏感性癫痫大鼠;注射干扰对照慢病毒的大鼠共10只成为慢性癫痫模型,最终筛选出3只耐药性癫痫大鼠,7只药物敏感性癫痫大鼠。结果分析,注射过表达慢病毒的大鼠耐药比率高于过表达对照组,而注射干扰慢病毒的大鼠耐药比率低于干扰对照组,见表2。 2.3 病毒注射结果 慢病毒转染后,镜下观察注射不同慢病毒大鼠的海马组织表达绿色荧光,提示各组大鼠慢病毒转染大鼠海马组织成功,慢病毒转染有效调控大鼠海马组织CREB表达,见图2。"
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