Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (29): 4681-4687.doi: 10.3969/j.issn.2095-4344.1814

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The secretion levels of various cytokines in human adipose mesenchymal stem cells under hypoxia

Wang Qian1, Liu Yi2, Zhang Yu3, Yang Yinxiang1, Wang Zhaoyan1, Zhang Leping1, Luan Zuo1   

  1. 1Department of Pediatrics, The Sixth Medical Center of PLA General Hospital, Beijing 100048, China; 2Department of Pediatrics, The First Medical Center of PLA General Hospital, Beijing 100039, China; 3Department of Neonates, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China
  • Revised:2019-05-20 Online:2019-10-18 Published:2019-10-18
  • Contact: Luan Zuo, Master, Chief physician, Department of Pediatrics, The Sixth Medical Center of PLA General Hospital, Beijing 100048, China
  • About author:Wang Qian, Technologist-in- charge, Department of Pediatrics, The Sixth Medical Center of PLA General Hospital, Beijing 100048, China； Liu Yi, Technologist-in- charge, Department of Pediatrics, The First Medical Center of PLA General Hospital, Beijing 100039, China. Wang Qian and Liu Yi contributed equally to this work.
  • Supported by:

    the Military Logistics Research Project, No. CHJ14C022 (to LZ)

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Abstract:

BACKGROUND: Mesenchymal stem cells derived from adipose tissue can secrete a variety of cytokines. Therefore, these cells have become hot seed cells for treating various ischemic diseases.
OBJECTIVE: To explore the effects of different oxygen partial pressures on the secretion of cytokines from human adipose mesenchymal stem cells, and to select the optimal oxygen partial pressures under which cytokines can highly express.
METHODS: Adipose mesenchymal stem cells were isolated from human adipose tissue by enzymatic digestion, and were identified by morphological characteristics, differentiation function and immunophenotype. Human adipose mesenchymal stem cells were cultured at 3%, 5%, and 21% partial oxygen pressures for 48 hours, respectively. The secretion levels of vascular endothelial growth factor, brain-derived neurotrophic factor, and insulin-like growth factor-1 in the cells were detected by enzyme-linked immunosorbent assay.
RESULTS AND CONCLUSION: Human adipose mesenchymal stem cells grew spirally with a long fusiform or polygonal shape, plump cell body, and uniform cytoplasm. They could be induced for adipogenic, osteogenic and chondrogenic differentiation in vitro. The cultured cells highly expressed mesenchymal surface markers CD29, CD73, CD90, and CD105, scarcely expressed hematopoietic stem cell surface markers CD34 and CD45, and negatively expressed HLA-DR. The level of vascular endothelial growth factors secreted by human adipose mesenchymal stem cells under 3% or 5% partial pressure of oxygen (hypoxia) was 2.3 times (P < 0.05) or 1.5 times (P < 0.05) as high as that under normoxia, respectively. The secretion level of insulin-like growth factor-1 under 3% or 5% partial pressure of oxygen (hypoxia) was 2.4 times (P < 0.05) or 1.5 times (P < 0.05)higher than that under normoxia, respectively. However, there was no significant difference in the secretion levels of brain-derived neurotrophic factor between hypoxic and normoxic conditions (P > 0.05). Therefore, human adipose mesenchymal stem cells significantly increase the secretion of vascular endothelial growth factor and insulin-like growth factor-1 under hypoxic conditions, while the secretion of brain-derived neurotrophic factor dose not change under hypoxic conditions.

Key words: hypoxia, human adipose stem cells, vascular endothelial growth factor, insulin-like growth factor-1, brain-derived neurotrophic factor, oxygen partial pressure

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