Isoliquiritigenin inhibits SETD7 expression against oxidative damage in cardiomyocytes induced by hypoxia/reoxygenation

doi:10.3969/j.issn.2095-4344.2353

Abstract

Abstract:

BACKGROUND: Ischemic heart disease has become one of the important diseases that threaten human health, but the molecular mechanism of its occurrence and development has not yet been elucidated.

OBJECTIVE: To investigate the effects of isoliquiritigenin (ISL) on oxidative stress and apoptosis in cardiomyocytes after hypoxia/reoxygenation (H/R) injury and its possible mechanism.

METHODS: Rat cardiomyocytes H9C2 were cultured in vitro and randomly divided into normal control group, H/R group, H/R+ISL 10, 20, 40, 80 μmol/L groups, H/R+si-con group, H/R+si-SETD7 group, H/R+ISL+pcDNA group, H/R+ISL+pcDNA-SETD7 group. Cell viability was measured using methylthiazolyl tetrazolium (MTT) assay. Apoptosis was detected by flow cytometry. The levels of reactive oxygen species (ROS), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) in the cells were measured. The expression of apoptosis-related protein Cleaved Caspase-3 was detected by western blot assay.

RESULTS AND CONCLUSION: Compared with the normal control group, the cell survival rate of H/R group was significantly decreased (P < 0.05), the apoptosis rate was significantly increased (P < 0.05), Cleaved caspase-3 expression and ROS and MDA levels were significantly increased (P < 0.05), while the GSH-Px content was significantly reduced (P < 0.05). Compared with the H/R group, the cell survival rate of H/R+ISL 10, 20, 40, 80 μmol/L groups was significantly increased (P < 0.05), and the apoptosis rate was significantly decreased (P < 0.05), and the expression of SETD7 and Cleaved caspase-3 was significantly decreased (P < 0.05), and the levels of ROS and MDA were significantly decreased (P < 0.05), while the content of GSH-Px was significantly increased (P < 0.05). Compared with the H/R+si-con group, the cell survival rate of H/R+si-SETD7 group was significantly increased (P < 0.05), the apoptosis rate was significantly decreased (P < 0.05), the expression of SETD7 and Cleaved caspase-3 was significantly decreased (P < 0.05), and the levels of ROS and MDA were significantly decreased (P < 0.05), while the content of GSH-Px was significantly increased (P < 0.05). Compared with the H/R+ISL+pcDNA group, the cell survival rate of H/R+ISL+pcDNA-SETD7 group was significantly decreased (P < 0.05), the apoptosis rate was significantly increased (P < 0.05), the expression of SETD7 and Cleaved caspase-3 was significantly increased (P < 0.05), and the levels of ROS and MDA were significantly increased (P < 0.05), while the content of GSH-Px was significantly decreased (P < 0.05). These findings indicate that ISL can inhibit the expression of SETD7 to reduce the apoptosis of cardiomyocytes induced by H/R and enhance the cell antioxidant capacity, thereby protecting cardiomyocytes against H/R injury.

Key words: ">isoliquiritigenin, SETD7, hypoxia/reoxygenation, cardiomyocytes, oxidative stress

CLC Number:

Yu Hui, Zhao Yang, Fei Jiayue, Zhang Wenli, Zhao Luosha. Isoliquiritigenin inhibits SETD7 expression against oxidative damage in cardiomyocytes induced by hypoxia/reoxygenation[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(35): 5613-5618.

Figures/Tables 5

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