Effect of Hippo-YAP signaling pathway on osteogenic differentiation of human amniotic epithelial cells

doi:10.12307/2023.697

Abstract

Abstract: BACKGROUND: Human amniotic epithelial cells, as a new type of seed cells, play an important role in bone injury repair.
OBJECTIVE: To investigate the potential regulatory mechanism of osteogenic differentiation of human amniotic epithelial cells in coculture with osteoblasts.
METHODS: Human amniotic epithelial cells were extracted by the enzyme digestion method. The proliferation of primary cells was described by the real-time cell analysis system xCELLigence RTCA S16. Human amniotic epithelial cells were co-cultured with osteoblasts by Transwell and corresponding samples were treated with siRNA-YAP1. The osteogenic differentiation of human amniotic epithelial cells was evaluated by detecting osteogenic associated proteins.
RESULTS AND CONCLUSION: (1) Primary human amniotic epithelial cells were adherent within 12 hours and entered an exponential growth period from 24 to 48 hours. (2) During coculture, human amniotic epithelial cells showed a trend of osteogenic differentiation, and the expression of YAP1 protein was increased in Hippo signaling pathway. (3) After siRNA-YAP1 was added during coculture, the expression of YAP1 protein in the Hippo signaling pathway was decreased and the osteogenic differentiation ability of human amniotic epithelial cells was weakened.

Key words: bone injury, human amniotic epithelial cell, osteogenic differentiation, cell coculture, signaling pathway

CLC Number:

Zhang Wenjing, Zhi Xiaodong, Zhang Yuqiang, Wang Wei. Effect of Hippo-YAP signaling pathway on osteogenic differentiation of human amniotic epithelial cells[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(24): 3788-3794.

Figures/Tables 6

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