Theaflavin-3,3'-digallate alleviates osteogenesis inhibition induced by wear particles in artificial prostheses

doi:10.12307/2023.411

Abstract

Abstract: BACKGROUND: Theaflavin-3,3'-digallate has shown a good therapeutic effect in animal models of osteoporosis by inhibiting osteoclast formation, but there are few studies on osteogenesis.
OBJECTIVE: To investigate the effect of theaflavin-3,3'-digallate on periprosthetic osteolysis.
METHODS: (1) In vitro test: The MC3T3-E1 cells with good growth status were selected and cultured in four groups. The control group was added with osteogenic differentiation medium; the titanium particle group was added with titanium particles + osteogenic differentiation medium; the low-dose group was added with titanium particles + theaflavin-3,3'-digallate (1 mg/L) + osteogenic differentiation medium; high-dose group was added with titanium particles + theaflavin-3,3'-digallate (10 mg/L) + osteogenic differentiation medium. After 2 weeks of osteogenic induction, alkaline phosphatase staining was performed; after 3 weeks of osteogenic induction, alizarin red staining was performed. (2) In vivo experiment: 32 C57BL/6J mice were randomly divided into 4 groups. The blank control group did not receive any treatment. In the model group, sterile titanium particles were evenly placed on the surface of the skull to establish an osteolysis model. The low-dose group and high-dose group were injected intraperitoneally with 1, 10 mg/(kg·d) theaflavin-3,3'-digallate into osteolysis model mice. The mouse skull was analyzed by micro-CT 2 weeks after operation.
RESULTS AND CONCLUSION: (1) In vitro experiments: alkaline phosphatase and alizarin red staining showed that titanium particles could inhibit alkaline phosphatase activity and mineralized nodule formation in MC3T3-E1 cells, and theaflavins-3,3'-bisgallate could inhibit the effect of titanium particles and was dose-dependent. (2) In vivo experiments: micro-CT scans showed that titanium particles could induce osteolysis around the skull, and high-dose theaflavin-3,3'-digallate could inhibit the osteolysis caused by titanium particles. (3) The results showed that theaflavin-3,3'-digallate could promote the osteogenic differentiation of MC3T3-E1 and inhibit periprosthetic osteolysis.

Key words: theaflavin-3,3'-digallate, titanium particle, osteogenic differentiation, periprosthetic osteolysis, prosthesis loosening

CLC Number:

Hou Zhenyang, Sun Yiling, Su Changzheng, Li Zhen, Xu Zhengwen, Li Wenming, Bai Jiaxiang. Theaflavin-3,3'-digallate alleviates osteogenesis inhibition induced by wear particles in artificial prostheses[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(25): 4069-4074.

Figures/Tables 3

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