Action mechanism and potential function on main components of organoid culture medium

doi:10.12307/2021.153

Abstract

Abstract: BACKGROUND: Traditional 2D cell culture has become the dominant method of cell culture due to its simplicity and easy operation. However, it is doubtful because of its inability to mimic the growth mode, microenvironment, and genetic stability of cells in the host. Organoid culture highly simulates the environment in the human body and recreates certain functions of the original organs. It is currently the most reliable cell model. However, due to the complexity and variety of the culture conditions, the organoid culture conditions are not yet mature. Therefore, it is very important to summarize the existing main culture components and improve the organoid culture technology on this basis.
OBJECTIVE: To study the main components and potential functions of various organoid culture media, providing theoretical basis for the culture of organoid establishment.
METHODS: The first author searched the related literature published from 2009 to 2020 in PubMed database, Cochrane Library database, CNKI database, and Wanfang database using the English or Chinese keywords “organoids, cell culture, cytokines, amino acid, R-spondin-1”. After excluding those repetitive and irrelevant articles to the purpose of this review, a total of 84 eligible articles were finally selected for review.
RESULTS AND CONCLUSION: In addition to the basic medium, organoid culture requires the participation of cytokines, amino acids, small molecule inhibitors and other substances. Different organoid culture requires different culture conditions, which is highly related to the source of the tissue. Each component plays a vital role in development of organoids from specific tissues. This article analyzes the detail role of each main culture component by summarizing the main existing organoid culture compound, which will provide a certain reference for the stable construction of various organoid models.

Key words: organoids, medium components, cytokines, amino acids, small molecule inhibitors, review

CLC Number:

Liu Qi, Yao Xi, Wei Zhengbo, Xie Ying. Action mechanism and potential function on main components of organoid culture medium[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(31): 5072-5078.

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