Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (10): 1545-1549.doi: 10.3969/j.issn.2095-4344.3026

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Safety and antibacterial properties of gentamicin-loaded silk fibroin in meniscus repair

Xu Changkui1, Pu Xiaobing2, Lu Yao3, Chen Jiarong4, Pan Lei1    

  1. 1Department of Spine and Joint, Sanshui District People’s Hospital of Foshan, Foshan 528000, Guangdong Province, China; 2Department of Orthopedics, West China Fourth Hospital, Sichuan University, Chengdu 610000, Sichuan Province, China; 3Department of Osteoarthritis, Zhujiang Hospital of Southern Medical University, Guangzhou 510000, Guangdong Province, China; 4Department of Orthopedics, General Hospital of Southern Theater Command, Guangzhou 510000, Guangdong Province, China
  • Received:2020-03-14 Revised:2020-03-19 Accepted:2020-05-09 Online:2021-04-08 Published:2020-12-18
  • Contact: Pan Lei, MD, Department of Spine and Joint, Sanshui District People’s Hospital of Foshan, Foshan 528000, Guangdong Province, China
  • About author:Xu Changkui, Master, Physician, Department of Spine and Joint, Sanshui District People’s Hospital of Foshan, Foshan 528000, Guangdong Province, China
  • Supported by:
    the Natural Science Foundation of Guangdong Province, No. 2014A030310147

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Abstract: BACKGROUND: Silk fibroin material has good biocompatibility, excellent mechanical properties, biodegradation and other advantages. However, it has not been used as a kind of suture fixation material in the repair of meniscus tear.
OBJECTIVE: To prepare gentamicin loaded silk fibroin material and analyze its biocompatibility and antibacterial properties.
METHODS: After dissolving the degummed natural silkworm cocoon with ternary solution, the silk fibroin material was prepared by dialysis, freeze-drying, redissolution and molding, and gentamicin was dissolved in silk fibroin solution to prepare gentamicin loaded silk fibroin material. Silk fibroin material and gentamicin loaded silk fibroin material were co-cultured with mouse pre chondroblasts respectively. After 1, 3, and 5 days, cell proliferation was detected by MTT assay. After 24 hours of co-culture, the cytotoxic effect of the material was detected by calcein staining. The early apoptosis of cells was detected by flow cytometry. The adhesion of cells on the surface of the material was observed by scanning electron microscope. Silk fibroin material and gentamicin loaded silk fibroin material were mixed with anticoagulant separately. The hemolysis rate of the material was detected by hemolysis experiment. The silk fibroin material and gentamicin loaded silk fibroin material were co-cultured with S.aureus and E.coli respectively. The bacterial count, scanning electron microscope observation and bacteriostatic circle experiment were carried out.  
RESULTS AND CONCLUSION: (1) The two materials had no significant effect on cell proliferation and early apoptosis. (2) There was no obvious cytotoxicity between the two materials, and the cells adhered on the surfaces of the two materials grew well, arranged tightly, and the morphology of the cells expanded. (3) The two materials had good blood compatibility and did not cause hemolytic reaction. (4) Gentamicin loaded silk fibroin material had obvious inhibition on S.aureus and E.coli, and the inhibition on S.aureus was more obvious. (5) The results showed that the gentamicin loaded silk fibroin material has good biocompatibility and antibacterial property.

Key words: bone, material, silk fibroin, meniscus, biocompatibility, antibacterial property, meniscus repair

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