Effect of icariin on the repair of femoral defects in rabbits by calcium phosphate bone cement/bone marrow mesenchymal stem cell complex

doi:10.3969/j.issn.2095-4344.1413

Abstract

Abstract:

BACKGROUND: Previous studies have shown that calcium phosphate bone cement/bone marrow mesenchymal stem cell complex can help repair cartilage defects. Icariin is a kind of flavonoids that can promote cell proliferation and differentiation.

OBJECTIVE: To investigate the effect of icariin-treated calcium phosphate cement/bone marrow mesenchymal stem cell complex in the repair of femoral defects in rabbits.

METHODS: Thirty New Zealand white rabbits were provided by Laboratory Animal Center, Hainan Medical University, China and prepared into models of right femur end defect. Then these models were randomly divided into three groups. In the blank group, there was no filling material for femoral defect. In the control group, the complex of calcium phosphate bone cement and the bone marrow mesenchymal stem cells which were cultured from osteoblasts was implanted in the femoral defect area. In the experimental group, the complex of calcium phosphate bone cement and the bone marrow mesenchymal stem cells which were cultured from osteoblasts and treated with icariin was implanted in the femoral defect area. Imaging and biomechanical analysis of bone defects were performed at 4, 8 and 12 weeks after surgery. Animal experiments were approved by the Ethics Committee, First Affiliated Hospital of Hainan Medical University, China.

RESULTS AND CONCLUSION: At postoperative 4, 8 and 12 weeks, micro-CT scans showed that the osteogenesis rate of the experimental group was significantly faster than that of the blank and control groups. At 12 weeks after surgery, some calcium phosphate bone cement was replaced by new bone, and callus was not completely enclosed in the control group. New bone formed in the femoral defect area, and the implanted calcium phosphate bone cement was completely degraded in the experimental group. At 12 weeks after surgery, trabecular mode factor, bone volume fraction, and the number of trabeculae in the experimental and control groups were significantly greater than those in the blank group (P < 0.05). The indexes in the experimental group were better than those in the control group (P < 0.05). At 4, 8 and 12 weeks after surgery, bone density in the experimental group was significantly higher than that in the control and blank group (P < 0.05). At 12 weeks after surgery, the maximum stress, maximum load and destructive energy in the experimental group were significantly greater than those in control and blank groups (P < 0.05). These results suggest that icariin-treated bone marrow mesenchymal stem cells/calcium phosphate bone cement complex can effectively promote new bone formation and accelerate bone repair.

Key words: calcium phosphate bone cement, icariin, bone marrow mesenchymal stem cells, femoral defect, bone density, biomechanics, bone formation, bone repair

CLC Number:

Jia Bingshen, Zhang Ximing, Yu Peng, Li Ming, Li Jun, Yun Dake, Wang Sheng, Hu Shuai. Effect of icariin on the repair of femoral defects in rabbits by calcium phosphate bone cement/bone marrow mesenchymal stem cell complex[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(30): 4757-4762.

Figures/Tables 6

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