Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (27): 4280-4285.doi: 10.3969/j.issn.2095-4344.1373

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Mechanism of long non-coding RNA in intervertebral disc degeneration

Zhang Chi1, Lü Haoyuan2, Zhang Xiaoyun3, Lin Zonghan3, Chen Yueping3, Liu Jianhang4, Dong Panfeng3, Chen Qingrong1   

  1.  (1Graduate School of Guangxi University of Chinese Medicine, Nanning 530001, Guangxi Zhuang Autonomous Region, China; 2Hubei University of Chinese Medicine, Wuhan 430061, Hubei Province, China; 3Department of Orthopedics, 4Department of Orthopedic Manipulation, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530011, Guangxi Zhuang Autonomous Region, China)
  • Received:2019-02-19 Online:2019-09-28 Published:2019-09-28
  • Contact: Zhang Xiaoyun, Master, Attending physician, Department of Orthopedics, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530011, Guangxi Zhuang Autonomous Region, China Corresponding author: Lin Zonghan, Chief physician, Master’s supervisor, Department of Orthopedics, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530011, Guangxi Zhuang Autonomous Region, China
  • About author:Zhang Chi, Master candidate, Graduate School of Guangxi University of Chinese Medicine, Nanning 530001, Guangxi Zhuang Autonomous Region, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81760796 (to CYP); the Discipline Training Project of Guangxi University of Chinese Medicine-Orthopedics Construction Project, No. 04B2017082 (to CYP); the Top Ten Engineering Projects of Chinese Medicine Development Planning, No. (2018)1 (to LJH); the School Qihuang Engineering Cultivation Project, No. 030030001-04131804804-500101 (to LJH)

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Abstract:

BACKGROUND: lncRNA GAS5 has been shown to activate the mitochondria apoptosis pathways in the nucleus pulposus cells of degenerative intervertebral disc, thus promoting pulposus apoptosis. 
OBJECTIVE: To explore the regulation mechanism of ceRNA network related to intervertebral disc degeneration and to find potential targets for the treatment of intervertebral disc degeneration.
METHODS: The GEO database was retrieved by computer, the lncRNA microarray chip GSE56081 was downloaded, the probe nucleic acid sequence in the platform file was re-annotated, and the probe ID in the matrix file of the chip series was converted into gene name by using Perl software, and the gene category was added. The R software was used to analyze the series of matrix files to obtain differential lncRNA and mRNA. Highly conserved miRNA family files were downloaded from the miRcode platform and compared to obtain lncRNA-miRNA associations. According to the miRDB database, the miRTarBase database and the TargetScan database, the miRNA-regulated mRNA was predicted, and the differential mRNA was obtained by the difference analysis of the chip data, finally the miRNA-mRNA associations were confirmed, and the ceRNA network was set up. By using the String database to analyze protein interactions, the key protein interaction modules were screened. The DAVID database was used to analyze the function and related pathways of the genes of the key protein modules, and to discover the key ceRNA networks.
RESULTS AND CONCLUSION: Differential lncRNA and mRNA competed for miRNA in degenerative intervertebral disc, which regulated the synthesis of protein and ultimately affected the ubiquitin-mediated proteolysis, Wnt signaling pathway, and PI3K-Akt signaling pathway. Seven miRNAs (hsa-miR-107, hsa-miR-449c-5p, hsa-miR-301b-3p, hsa-miR-135a-5p, hsa-miR-17-5p, hsa-miR-20b-5p, hsa-miR-876-3p) are found which may play an important role in the protein catabolism and apoptosis, leading to degeneration of the intervertebral disc.

Key words:  intervertebral disc degeneration, ceRNA, lncRNA, miRNA, protein interaction network, microarray analysis, data mining, bioinformatics

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