Potential molecular targets and therapeutic mechanisms underlying transplantation of autologous bone marrow stem cells for the treatment of spinal cord injury based on bioinformatics

doi:10.3969/j.issn.2095-4344.2131

Abstract

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells have achieved good clinical effect in the treatment of spinal cord injury. However, most of the existing studies focus on the pathophysiological changes, and less is reported on the molecular regulatory mechanism involved.

OBJECTIVE: To study the potential molecular targets and signal pathway related to the use of bone marrow mesenchymal stem cells in the treatment of spinal cord injury based on bioinformatics analysis.

METHODS: The microarray data of bone marrow mesenchymal stem cells for spinal cord injury treatment were retrieved from GEO database and then mapped to the background network of spinal cord injury obtained from CTD database. Finally, the differentially expressed genes were obtained. A protein-protein interaction network constructed by sting was used to analyze its network characteristics using Cytoscape and obtain the core molecular targets in the process. Finally, the molecular functions and signal pathways of the above molecular targets were analyzed by functional enrichment of the above genes on the website of WebGestalt.

RESULTS AND CONCLUSION: According to the data of GEO chip, there are 126 core molecular targets in the treatment of spinal cord injury by bone marrow mesenchymal stem cells, 60 of which have been reported in CTD database. According to the results of sting and Cytoscape, there are 57 nodes in the protein-protein interaction network, among which 10 core targets are MAPK3, tumor necrosis factor, interleukin 6, CASP3, vascular endothelial growth factor A, MAPK1, CCL2, interleukin 1B, proto-oncogene protein, insulin-like growth factor 1. In this process, the main regulatory pathways are interleukin 17, MAPK, tumor necrosis factor, Toll-like receptor and nod-like receptor. The two most important aspects of this process are the inhibition of inflammatory response mediated nerve damage and the nutritional repair of damaged nerve.

Key words: bioinformatics, spinal cord injury, stem cell therapy, bone marrow mesenchymal stem cells, molecular mechanism, protein-protein interaction network, neuroprotection, neural repair

CLC Number:

Xun Chong, Wang Qiang, Li Changzhou, Liu Xiaofeng. Potential molecular targets and therapeutic mechanisms underlying transplantation of autologous bone marrow stem cells for the treatment of spinal cord injury based on bioinformatics[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(31): 4927-4933.

Figures/Tables 6

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