Titanium-copper alloys with nanotubular coatings increase antibacterial abilities and osteoblast functions

doi:10.3969/j.issn.2095-4344.0692

Abstract

Abstract:

BACKGROUND: Implant infection is still a serious adverse event after orthopedic surgery. Copper (Cu) is a currently known metal that has antibacterial properties. Studies have shown that nanostructured metals prepared by nanotechnology can promote the adhesion, proliferation and osseointegration of osteoblasts in vivo. Staphylococcus aureus, the most common implant-related pathogen in clinical practice, is used to test antibacterial properties of titanium-copper alloy nanotubes.

OBJECTIVE: To observe the effect of antibacterial properties of titanium-copper alloy nanotubes on the function of osteoblasts.

METHODS: Mouse osteoblasts (MC-3T3-E1) were co-cultured with different materials, including pure titanium, titanium dioxide nanotubes, and titanium-copper alloy nanotubes with a copper content of 5%, for 6 and 24 hours. Cell adhesion and proliferation on the scaffold were observed. Antibacterial properties of titanium-copper alloy nanotubes, titanium dioxide nanotubes and pure titanium were compared. Biocompatibility of osteoblasts co-cultured on different material surfaces was detected, and antibacterial properties of different materials to Staphylococcus aureus were measured.

RESULTS AND CONCLUSION: (1) Under scanning electron microscope, we observed good cell adhesion onto the surface of titanium dioxide nanotubes and titanium-copper alloy nanotubes, and the adherent cells had good cell morphology and regular arrangement. Cell proliferation of osteoblasts was better in the two nanotube groups than in the pure titanium group, but there was no significant difference between the titanium-copper alloy nanotube and titanium dioxide nanotube groups. (2) Higher bacteria counts were observed in the pure titanium and titanium dioxide nanotube groups than the titanium-copper alloy nanotube group. In conclusion, the titanium-copper alloy nanotubes have good inhibitory effect on bacterial adhesion, and have no influence on the bio-functions of osteoblasts.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Tissue Engineering, Titanium, Staphylococcus aureus, Osteoblasts

CLC Number:

R496
R318

Zhang Lei, Zhang Hangzhou, Allieu Kamara. Titanium-copper alloys with nanotubular coatings increase antibacterial abilities and osteoblast functions[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(2): 165-171.

Figures/Tables 5

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