A rat model of liver cirrhosis induced by the combination of carbon terachloride and ethanol: establishment and verification

doi:10.3969/j.issn.2095-4344.0813

Abstract

Abstract:

BACKGROUND: Establishing a stable, reliable, easily handling and highly efficient animal model of liver cirrhosis is the prerequisite for the study on the prevention and treatment of the disease.
OBJECTIVE: To establish and evaluate an ideal means to induce liver cirrhosis in rats by carbon tetrachloride (CCl4).
METHODS: Wistar rats were randomly allotted into control group (n=8), group A (n=21) and group B (n=21). Olive oil was injected subcutaneously into both sides of the abdomen of control rats every 4 days; in the group A, 50% CCl4 in olive oil was injected subcutaneously into the abdomen with a dosage of 3 mL/kg every 4 days, and a 10% ethanol solution (Erguotou liquor and distilled water) constituted the only liquid drank; in the group B, 50% CCl4 in olive oil was injected subcutaneously into the abdomen every four days, the first four and final three doses were 5 mL/kg, and the other doses were 3 mL/kg, and a gradually transition from 5% to 10% ethanol solution (Erguotou liquor and distilled water), constituted the only liquid drank. The modeling time was 9 weeks.
RESULTS AND CONCLUSION: There was a slower increase in the rat body mass in the groups A and B compared with the control group, especially in the group B. The serum levels of alanine aminotransferase and aspartate aminotransferase in the groups A and B were significantly higher than those in the control group. The level of alanine aminotransferase in the group B was significantly higher than that in the group A, but there was no significant difference in the aspartate aminotransferase level between the groups A and B. The cirrhosis with pseudolobules rate and success rate in the group B were higher than those in the group A. There was no significant difference in survival rate between group B and control group. That is to say, the improved method of inducing cirrhotic model in rats by CCl4 combined with ethanol shows a high rate of success and a low rate of mortality.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Liver, Liver Cirrhosis, Carbon Tetrachloride, Tissue Engineering

CLC Number:

R318

Bin Wen-ting1, Chang Jia-song1, Wu Jian-ping2. A rat model of liver cirrhosis induced by the combination of carbon terachloride and ethanol: establishment and verification[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(20): 3224-3229.

Figures/Tables 1

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