Changes of perivascular astrocyte foot process in the cerebral cortex of a mouse model of sustained hyperglycemia

doi:10.3969/j.issn.2095-4344.0288

Abstract

Abstract:

BACKGROUND: Sustained hyperglycemia can induce cerebral microvascular disease and peripheral neuropathy, which significantly increases the risk of stroke and neurological dysfunction. Astrocyte foot process around the cerebral microvessels may play a vital role in the pathogenesis of sustained gyperglycemia.
OBJECTIVE: To observe the changes of perivascular astrocyte foot process and gliovascular interface in cerebral cortex of mice with sustained hyperglycemia, and to investigate the mechanism of hyperglycemia-induced cerebral microvascular diseases.
METHODS: Twenty healthy adult male Kunming mice were randomly divided into two groups: control group and hyperglycemia group. The hyperglycemia was induced by intraperitioneal injection of streptozotocin (50 mg/kg) for 5 consecutive days. The expression levels of aquaporin-4 in the astrocyte foot process and collagen type IV in the vascular basement membrane were detected by double immunofluorescent staining to observe the changes in perivascular astrocyte foot process and gliovascular interface. The expression levels of aquaporin-4 in the astrocyte foot process and collagen type IV in the vascular basement membrane were determined by immunohistochemistry.
RESULTS AND CONCLUSION: Compared with the control group, in the hyperglycemia group, there was a significant decrease in the colocalization of aquaporin-4 in the astrocyte foot process and collagen type IV in the vascular basement membrane (P < 0.05), and the expression levels of aquaporin-4 and collagen type IV were significantly decreased (P < 0.05). Immunohistochemistry results showed that the expression levels of aquaporin-4 in the astrocyte foot process and collagen type IV in the vascular basement membrane in the hyperglycemia group were significantly lower than those in the control group (P < 0.05). These results indicate that sustained hyperglycemia can destroy gliovascular interface, and result in neurovascular unit and blood brain barrier dysfunction, probably by reducing the expression level of aquaporin-4 in the astrocyte foot process.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Hyperglycemia, Basilar Membrane, Models, Animal, Tissue Engineering

CLC Number:

R318

Huang Yan1, He Jing1, Xiang Yang2, Wang Zhi-qiang2, Du Guo2, Wang Qing-song1, 2. Changes of perivascular astrocyte foot process in the cerebral cortex of a mouse model of sustained hyperglycemia[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(20): 3218-3223.

Figures/Tables 1

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