Chinese Journal of Tissue Engineering Research ›› 2017, Vol. 21 ›› Issue (26): 4210-4216.doi: 10.3969/j.issn.2095-4344.2017.26.019
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Received:
2017-04-19
Online:
2017-09-18
Published:
2017-09-28
Contact:
Hou Xiao-wei, Professor, Department of Prosthodontics, the Third Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China
About author:
Sun Zhi-hui, Studying for master’s degree, Department of Prosthodontics, the Third Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China
CLC Number:
Sun Zhi-hui, Zheng Yu, Wang Li-jie, Fan Shi-feng, Hou Xiao-wei.
2.1 实验动物数量分析 9只兔均进入结果分析。 2.2 大体观察结果 所有术后实验动物均健康无死亡,术后1周创口愈合良好。定期检查见各时段植区周围牙龈无红肿、无破溃、无溢脓炎症表现。 9只实验动物共得36个标本,所有标本钛螺纹钉均无脱落。实验组钛螺纹钉未见松动现象,钛螺纹钉周围骨组织紧密包绕,未见纤维组织长入。对照组植入4周,部分钛螺纹钉松动,钛螺纹钉龈方周围存在角形骨缺损;植入8周,钛螺纹钉松动度有好转,骨缺损面积减小,植入12周,钛螺纹钉顶端周围有骨组织包绕。 2.3 X射线观察及骨密度分析结果 X射线观察结果:X射线片可见各标本钛螺纹钉均在位,两组钛螺纹钉周围新生骨量均随时间延长而升高。植入后4周时,两组拔牙窝内异种冻干骨与周围骨组织均无明显边界,实验组可见钛螺纹钉周围新生骨影像呈云雾状,包绕钛螺纹钉整体,外周皮质骨密度无降低;对照组钛螺纹钉周围较大透射区,新生骨云雾状不明显且钛螺纹钉顶端见骨缺损区。植入后8周时,两组钛螺纹钉-骨界面密度较4周均有明显升高,实验组新生骨量增多,密度与正常骨密度接近,新生骨界限不明显;对照组钛螺纹钉顶端仍可见透射影像,新生骨密度较低且界限清晰。植入后12周,实验组可见新生骨组织紧密包绕在螺纹钉周围,密度较正常骨密度稍高;对照组龈方透射影像基本消失,新生骨密度较植入8周时增加但仍低于正常骨组织,新生骨界限仍可分辨,见图1。 骨密度结果分析:实验组各时间点钛螺纹钉周围骨组织的灰度值均高于对照组(P < 0.05),见表1。"
2.5 组织学观察结果 植入后4,8,12周标本组织学观察显示,实验组成骨细胞较为活跃,新生骨与成熟骨出现较早。 实验组:植入后4周,可见成骨细胞活跃,在新生骨小梁周围紧密排列,钛螺纹钉周围区域可见骨吸收陷窝及类骨质分泌,新生骨小梁呈片状分布,可见编织骨形成,视野内见丰富的新生血管。植入后8周,成骨细胞数量减少,骨细胞增多,破骨细胞几乎不可见,大量类骨质矿化为骨基质,骨小梁较4周排列密集、粗大,可见编织骨转变成板状新骨。植入后12周,可见骨细胞较多,排列紧密,骨小梁排列粗大致密,大量板状新骨形成,见图2。 对照组:植入后4周,见大量结缔组织细胞成分,成骨细胞量少,破骨细胞较多,但形成的骨吸收陷窝较少,类骨质分泌少,骨小梁几乎不可见,新生血管极其稀疏。植入后8周,可见成骨细胞较4周增多,破骨细胞减少,类骨质沉积较实验组低,新生骨小梁纤细、稀疏,新生血管较4周增多。植入后12周,骨细胞排列混杂,骨小梁较实验组纤细,骨成熟度较低,仅有少量板状新骨形成,见图2。"
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