Repair of abdominal wall defects by highly simulated chitosan scaffolds

doi:10.3969/j.issn.2095-4344.2017.26.018

Abstract

Abstract:

BACKGROUND: Studies have shown that chitosan can improve the proliferation and migration of fibroblasts, promote the synthesis of extracellular matrix, and create a favorable environment for defect healing.

OBJECTIVE: To observe the effect of highly simulated chitosan scaffolds in repairing abdominal wall defects in rats.

METHODS: Sixty adult Sprague-Dawley rats were used to make animal models of abdominal wall defects, and then model rats were randomly divided into two groups: experimental group and control group followed by implantation with the highly simulated chitosan scaffold and polypropylene mesh, respectively. At 2, 4 weeks after repair, gross, adhesion and histopathological observations of the repair part were performed.

RESULTS AND CNCLUSION: Gross observation: in the control group, omental adhesion was found at 2 weeks after repair; and at 4 weeks after repair, the mesh was thickened and exhibited clear boundaries with the surrounding tissue, but common color and integration. In the experimental group, there was mild adhesion between the scaffold and the defect site at 2 weeks after repair, and the mesh was thickened, but the wound healed well with no prominence, swelling, and infection at 4 weeks after repair. Kadata adhesion score in the experimental group was lower than that the control group at 4 weeks after repair (P < 0.05). Pathological observation showed that at 4 weeks after repair, there were a large number of infiltrated inflammatory cells, capillary growth, and a large number of fibroblasts at the defect site in the control group; and meanwhile, a small amount of infiltrated inflammatory cell infiltration and a large number of collagen fibers and capillaries, with better granulation tissue maturity, were found in the experimental group. To conclude, the highly simulated chitosan scaffold can promote the repair of abdominal wall defects and reduce inflammatory reactions.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Chitosan, Polypropylenes, Tissue Adhesions, Tissue Engineering

CLC Number:

R318

Liu Hong-ze, Wei Jie.

Repair of abdominal wall defects by highly simulated chitosan scaffolds

[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(26): 4205-4209.

Figures/Tables 5

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