Accurate determination of HLA ambiguous results based on group-specific haploid full-length sequencing

doi:10.3969/j.issn.2095-4344.2017.20.016

Abstract

Abstract:

BACKGROUND: Due to the polymorphism of HLA, a large number of ambiguities have been generated by conventional HLA typing techniques, and confirmed stereotypes of ambiguous results based on group-specific haploid full-length typing are rarely reported.

OBJECTIVE: To analyze the accuracy of HLA-typing ambigulity based on group-specific haploid full-length sequencing.

METHODS: The low-resolution results were used as the starting point for two ambiguous samples. Sanger sequencing (PCR-SBT) based on haploid full-length was performed after group-specific amplification.

RESULTS AND CONCLUSION: One case showed a new A*02:03:01 allele, which was found a mutation in NT817 from C to T in comparison with A*11:01:01:01. The other case indicated another new C*07:02:01:01, which was found a mutation in NT879 from A to G in comparison with C*08:01:01. In conclusion, these results indicate that the group-specific haploid full-length sequencing method can be used to accurately classify HLA alleles and to discover new alleles.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: HLA Antigens, Genes, Tissue Engineering

CLC Number:

R318

Wang Song-xing, Yang Hui, He Liu-mei, Hong Wen-xu, Zou Hong-yan, Xu Yun-ping. Accurate determination of HLA ambiguous results based on group-specific haploid full-length sequencing[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(20): 3208-3215.

Figures/Tables 1

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