Table of Content

18 July 2017, Volume 21 Issue 20 Previous Issue    Next Issue

For Selected:

Download Citations EndNote Reference Manager ProCite BibTeX RefWorks

Toggle Thumbnails

Select

Polygonatum sibiricum polysaccharide attenuates bone marrow-derived macrophages to differentiate into osteoclasts and protects against lipopolysaccharide-induced osteolysis in vivo He Ji-chen1, Zong Shao-hui1, Zeng Gao-feng2, Du Li1, Peng Xiao-ming1, Shi Xiong-zhi1, Wu Yun-le1 2017, 21 (20):  3117-3122.  doi: 10.3969/j.issn.2095-4344.2017.20.001 Abstract ( 345 )   PDF (940KB) ( 571 )   Save BACKGROUND: Bone marrow-derived mononuclear cells (BM-MNCs) hold the potential of differentiating into osteoclasts. Polygonatum sibiricum polysaccharide (PSP) may inhibit the differentiation of BM-MNCs into osteoclasts and it is expected to become a new drug for the treatment of osteoporosis. OBJECTIVE: To investigate the effect of PSP on the differentiation of mouse BM-MNCs into osteoclasts induced by receptor activator of nuclear factor kappa-B ligand (RANKL) and bone resorption in vivo. METHODS: Mouse bone marrow-derived macrophages cultured in vitro, the effect of macrophage colony stimulating factor and PSP (5, 10, 20, 40, 80,160, 320, 640, 1 280, 2 560 mg/L) on the proliferation of mouse BM-MNCs was detected by cell counting kit-8 assay to determine the PSP concentration range; the mouse BMMs were cultured and induced in DMEM medium containing macrophage colony stimulating factor, RANKL and 5, 10, 20, 40, 80,160, 320, 640 mg/L PSP, respectively; those cultured without PSP served as control group. The morphological changes of cells were observed under an inverted microscope.; the number of osteoclasts was detected by tartrate-resistant acid phosphatase staining; the mRNA expression levels of osteoclast-related genes including tartrate-resistant acid phosphatase, matrix metalloproteinase-9, cathepsin K, and nuclear factor of activated T cells c1 were evaluated by quantitative real-time PCR. A mouse model of calvarial osteolysis induced by lipopolysaccharide was established to receive PSP intervention, and then micro CT scanning, three-dimensional reconstruction and relevants software were used for quantitative analysis of bone volume/volume percentage, trabecular number, trabecular bone spacing and thickness. The number of osteoclasts was identified by tartrate-resistant acid phosphatase staining and quantitative analysis of bone resorption area was conducted. RESULTS AND CONCLUSION: Compared with the control group, the concentration of PSP below 640 mg/L showed no significant effect on the proliferation of BMMs (P > 0.05). Different concentrations of PSP (40-640 mg/L) significantly reduced the number of osteoclasts, osteoclast differentiation and maturation, and the mRNA expression levels of tartrate-resistant acid phosphatase, matrix metalloproteinase-9, cathepsin K, and nuclear factor of activated T cells c1 TRAP, MMP-9, CtsK and NFATc1 (P < 0.05). Compared with lipopolysaccharide, PSP could effectively alleviate the lipopolysaccharide-induced calvarial osteolysis, and the bone volume/volume percentage, trabecular number, and trabecular bone spacing were significantly decreased (P < 0.05); additionally, the number of osteoclasts and the area of bone resorption were decreased significantly (P < 0.01). To conclude, PSP can inhibit the differentiation and maturation of mouse BMMs to osteoclasts and alleviate lipopolysaccharide-induced calvarial osteolysis. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Radiofrequency combined with platelet-rich plasma for repair of white meniscal tears Zhou Wei-feng, Zhu Lin, Jiang Xue-feng, Wang Ya-bin 2017, 21 (20):  3123-3128.  doi: 10.3969/j.issn.2095-4344.2017.20.002 Abstract ( 300 )   PDF (818KB) ( 238 )   Save BACKGROUND: Both biological and physical methods contribute to the repair of white meniscal tears, so the combined use will be better. OBJECTIVE: To study the influence of radiofrequency combined with platelet-rich plasma (PRP) on the repair of white meniscal tears. METHODS: Forty-eight adult New Zealand white rabbits were randomly divided into four groups, including simple suture, radiofrequency, PRP, and combination groups, and then the model of medial meniscus posterior root tears was established in all experimental animals. The simple suture group underwent interrupted mattress suture; the radiofrequency group was treated with radiofrequency (20 W, 45 ℃) after suture; the PRP group received the intra-articular injection of PRP after suture; the combination group was firstly treated with radiofrequency after suture, and then underwent the intra-articular injection of PRP. The gross and histological changes of the meniscus were observed, and the expression levels of transforming growth factor β1, vascular endothelial growth factor and platelet-derived growth factor were detected at 3 and 12 weeks postoperatively. RESULTS AND CONCLUSION: At 12 weeks after operation, the simple suture group showed no healing; the radiofrequency and PRP groups healed partially; and the combination group healed completely, and chondrocytes and collagen fibers arranged regularly. There was a significant difference in the healing rate between combination and simple suture groups (P=0.003). At 3 weeks postoperatively, the expression levels of vascular endothelial growth factor in the radiofrequency, PRP and combination groups were obviously higher than that in the simple suture group, which decreased markedly at the 12th week. At 3 weeks postoperatively, the expression level of platelet-derived growth factor was increased in all groups, especially in the PRP and combination groups. The PRP group showed the highest level of transforming growth factor β1 at 12 weeks postoperatively. These results manifest that the combination of radiofrequency and PRP promotes the repair of white meniscal tears by increasing the cell proliferation, cell mitosis and angiogenesis.     中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Effect of Trifolium pratense L. extracts on the bone resorption and osteogenic differentiation of osteoclasts and the underlying mechanisms Li Yang, Wang De-ping, Ai Dong, Wei Xin, Guan Li-xin, Guan Yue 2017, 21 (20):  3129-3134.  doi: 10.3969/j.issn.2095-4344.2017.20.003 Abstract ( 310 )   PDF (1632KB) ( 360 )   Save BACKGROUND: Isoflavone isolated from Trifolium pratense L. has been found to be able to effectively inhibit bone resorption, reduce bone turnover rate, improve osteocyte activity and bone mineral density by enhancing the effect of estrogen, which is helpful for the prevention and treatment of osteoporosis. OBJECTIVE: To investigate the effect of Trifolium pratense L. extracts on the bone resorption and differentiation of osteoclasts. METHODS: Rat bone marrow cells were extracted, isolated by lymphocyte separation and cultured for 5 hours; then, the non-adherent cells were selected followed by induced by 30 μg/L macrophage colony stimulating factor and 75 μg/L RANKL (control groups), or different concentrations of Trifolium pratense L. extracts (0.3, 0.6 and 1.2 g/L) to observe their effect on the osteoclast differentiation and bone resorption. The levels of osteoclast differentiation-associated proteins c-fos and NFATcl were determined by western blot assay. RESULTS AND CONCLUSION: Compared with the control group, different concentrations of Trifolium pratense L. extracts could suppress osteoclast differentiation and bone resorption to different degrees. Tartrate-resistant acid phosphatase staining showed that Trifolium pratense L. extracts could significantly reduce the number of osteoclasts. Western blot assay results suggest that Trifolium pratense L. extracts significantly inhibited the expression levels of c-fos and NFATcl. These results reveal that Trifolium pratense L. extracts can inhibit osteoclast differentiation and bone resorption.     中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Expression and role of H-type vessels in the subchondral bone in the progression of osteoarthritis Lu Jian-sen, Liu Xin, Zeng Chun, Zhao Chang, Fang Hang, Cai Dao-zhang 2017, 21 (20):  3135-3140.  doi: 10.3969/j.issn.2095-4344.2017.20.004 Abstract ( 609 )   PDF (2036KB) ( 347 )   Save BACKGROUND: H-type vessels are mainly distributed in the metaphysis, which can promote the proliferation of osteocytes, further accelerating osteogenesis. OBJECTIVE: To investigate the expression of H-type vessels in the subchondral bone during the occurrence and development of osteoarthritis. METHODS: 8-week-old C57 mice were randomly divided into experimental and sham groups, followed by the right medial menisectomy to establish the osteoarthritis models or only articular capsulotomy. The knee samples were removed at 4 weeks postoperatively, and were stained with safranin-O-fast green to evaluate the degree of injury. The expression levels of CD31, Emcn and matrix metalloproteinase 13 were detected by immunofluorescent staining. The changes of the subchondral bone were observed by hematoxylin-eosin staining and the changes of bone mass in the subchondral bone were analyzed by micro-CT. RESULTS AND CONCLUSION: Compared with the sham group, the Osteoarthritis Research Society International scores, expression levels of CD31, Emcn, H-type vessels and matrix metalloproteinase 13, as well as the bone mass in the subchondral bone were significantly increased in the experimental group (P < 0.05). To conclude, the increased H-type vessels in the subchondral bone promote the hyperplasia and remodeling of subchondral bone in the progression of osteoarthritis.     中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Morphological features of the knee joint in healthy Zhuang ethnic group from Guangxi Zhuang Autonomous Region, China Zhang Xiao-yun, Chen Yue-ping, Xia Tian, Kang Jie, Zhuo Ying-hong, Hu Qing-lei, Li Jin-huan, Dong Pan-feng,Zhao Bin, Cao Zhen-bin 2017, 21 (20):  3141-3146.  doi: 10.3969/j.issn.2095-4344.2017.20.005 Abstract ( 241 )   PDF (1716KB) ( 198 )   Save BACKGROUND: With the increasing number of patients undergoing knee arthroplasty in China, the complications caused by the mismatch of prosthesis with anatomical parameters arouse extensive attentions. OBJECTIVE: To measure the anatomical parameters of the knee joints in healthy Zhuang ethnic group from Guangxi Zhuang Autonomous Region, China, using three-dimensional (3D) reconstruction technology, thus providing reference for the design and placement of the hip prosthesis. METHODS: Sixty-eight individuals, including 30 females and 38 males, were selected from 217 healthy people undergoing CT examination. All data were imported into Mimics 15.0 software used for 3D reconstruction, and then the knee parameters were then measured and analyzed. RESULTS AND CONCLUSION: There was no significant difference in the parameters of the bilateral femur and tibia (P > 0.05). The femoral parameters had significant differences between genders (P < 0.05). The width of tibial platform, sagittal length of lateral tibial platform, distance between medial tibial plateau and fibular head, and posterior slope of tibial plateau showed no significant differences between genders (P > 0.05), while there were significant differences in the width of medial/lateral tibial platform, and sagittal length of medial tibial platform between genders (P < 0.05). The sagittal length of medial femoral condyle was negatively correlated with age, and all femoral parameters were positively correlated with height; while only the width of medial and lateral tibial platform were positively correlated with height. To conclude, 3D reconstruction technology is available for research on the morphology of the knee joint in the Zhuang ethnic group from Guangxi Zhuang Autonomous Region, which provides references for prosthesis design and research appropriate for the Zhuang ethnic group. Figures and Tables | References | Related Articles | Metrics

Select

Micromechanical properties of articular cartilage resilience under different compression conditions Xu Xi-fan, Gao Li-lan, Men Yu-tao, Dong Pei-bei, Zhang Chun-qiu 2017, 21 (20):  3147-3151.  doi: 10.3969/j.issn.2095-4344.2017.20.006 Abstract ( 336 )   PDF (935KB) ( 271 )   Save BACKGROUND: It is of great significance to study the resilience of articular cartilage for human daily routine  and their match quality. OBJECTIVE: To analyze the micromechanical properties of articular cartilage resilience under different loads and at time points. METHODS: The swine cartilage samples coated with tracers were compressed using the MTF-100 tensile machine, and the cartilage compression and resilience were recorded by CCD. Images were processed using digital image correlation technology. RESULTS AND CONCLUSION: During resilience, the strain value on the superficial surface of the cartilage was decreased most, successively followed by the middle layer and the deep layer, while the time of a decrease from 20%, 10% and 6% to 3% was similar. The longer the resilience time was, the more slowly the strain changed in different layers of the cartilage, but the ultimate strain was less than 1%. On the same layer under different compressive stress, the larger load caused faster strain change firstly, and then the smaller load brought about faster strain change. The effect of different continuous compressive time on the same layer of cartilage was similar with the load. These results showed that 90% resilience of the articular cartilage occurred within the first 15 minutes. The mechanical resilience of different layers of the articular cartilage has a close relationship with the loading and the loading time, and both compressive time and loading do harm to the resilience of articular cartilage. Besides, the cartilage will rebound to the state before compression.   中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Orthopantomography and cone-beam CT for bone height measurement and simulation in posterior implant region Lu Xi, Su Yi 2017, 21 (20):  3152-3157.  doi: 10.3969/j.issn.2095-4344.2017.20.007 Abstract ( 463 )   PDF (1065KB) ( 347 )   Save BACKGROUND: Cone-beam CT (CBCT) and orthopantography are two imaging methods mostly used in the preoperative evaluation of dental implantation. CBCT has high cost and low penetration rate, but orthopantography can result in a larger error in the measurement of bone quantity. OBJECTIVE: To compare the accuracy of bone quantity measurement using CBCT and orthopantography, and to evaluate the clinical effect of simulation in posterior implant region. METHODS: 115 implants from 72 patients undergoing implantation of posterior teeth in the Eye & ENT Hospital of Fudan University were selected. The horizontal heights from the top of the alveolar bone to the important anatomic structures (mandibular nerve or maxillary sinus floor) were measured using CBCT and orthopantography and then analyzed statistically. The implantation simulations were performed using Planmeca Romexis3.8 (for CBCT) and Cliniview9.3 (for orthopantography) to compare the differences in stimulated and postoperative horizontal heights. RESULTS AND CONCLUSION: There were significant differences in the measured data between CBCT and orthopantography (P < 0.05), especially in the maxilla (P < 0.01). There were significant differences between the simulated and postoperative results of the two methods (P < 0.05). The average error and standard deviation in orthopantography were larger than those in CBCT. The average error of implantation simulation using CBCT in the maxilla was smaller than that using orthopantography, whereas the error was similar in mandibular simulations. To conclude, CBCT is more accurate for assessing the quantity of bone in posterior implant region, especially in the maxilla. The accuracy of CBCT and orthopantography in implantation simulation needs to be improved.     中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Reconstruction of the medial patellofemoral ligament in the treatment of adolescent acute patellar dislocation using “double-pulley” technique Yuan Ling-li, Xu Bin, Xu Wen-di, Geng Chun-hui 2017, 21 (20):  3158-3163.  doi: 10.3969/j.issn.2095-4344.2017.20.008 Abstract ( 284 )   PDF (954KB) ( 227 )   Save BACKGROUND: Although there are many studies on acute patellar dislocation in adolescents, the optimal treatment strategy is under discussion. OBJECTIVE: To summarize the clinical efficacy of reconstruction of the medial patellofemoral ligament (MPFL) in the treatment of adolescent acute patellar dislocation using “double-pulley” technique. METHODS: Thirty cases of acute patellar dislocation were enrolled and received reconstruction of the MPFL using “double-pulley” technique. We proceeded to prepare the bone bed for anchor placement at the medial point of the superior edge of the patella and at the upper 1/3 patella along the long axis of the patella using arthroscopic grinding. Two double-loaded 5.0 anchors were respectively placed at the medial point of patella and at the upper 1/3 patella parallel to the patellar articular surface, and then tied using the double-pulley technique. Long longitudinal incisions were performed at the medial condyle and at the adductor tubercle to expose these two structures, the deep fascias tunnel between two incisions were penetrated to pull the tendon weave end through the tunnel until the lateral side along a femoral tunnel; finally the tendon was fixed using screws in appropriate tightness. All patients were followed for more than 10 months, and the patellar-related parameters and knee function parameters were compared before and after operation. RESULTS AND CONCLUSION: There were 12 cases of intra-articular cartilage injury and 4 cases of meniscus injury. The patella title angle, Q-angle and outward shift distance at the last follow-up were less than those before operation. Lysholm and International Knee Documentation Committee scores at the last follow-up were higher than those before operation. The mean operation time was (90±10.5) minutes. There was one patient with knee movement angle from 0° to 30° and one patient with a patellar fracture caused by slipping and treated with internal fixation. None of patients appeared with patellar dislocation, positive apprehension test infection or poor wound healing. These results indicate that the simple and mini-invasive double-pulley technique is a good treatment strategy for acute patellar dislocation, because it is consistent with patellar anatomical stability, holds patellar trajectory visibility, quick recovery and good curative effect. Additionally, osteotomy is recommended when the simple efficacy is not satisfactory.     中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Different dosages of retinoic acid to establish a rat model of osteoporosis: a stability evaluation Sun Shi-dong, Liang Qi-bin, Fan Wei-zhi, Zeng Zhan-peng, Chen Bo-xing 2017, 21 (20):  3164-3169.  doi: 10.3969/j.issn.2095-4344.2017.20.009 Abstract ( 279 )   PDF (887KB) ( 543 )   Save BACKGROUND: Osteoporosis is a serious threat to the health and quality of life in the elderly. It is important to establish an ideal experimental animal model to study the etiology and treatment of osteoporosis. OBJECTIVE: To establish a rat model of osteoporosis induced by different dosages of retinoic acid, thus selecting the optimal dosage.   METHODS: Eighty female Sprague-Dawley rats were randomly divided into control, low-, middle- and high-dosage groups based on body mass (n=20 per group), The rats in the latter three groups were induced with 80, 100, and     120 mg/(kg•d) retinoic acid via gastric lavage for 14 days. RESULTS AND CONCLUSION: Compared with the control group, the bone mineral density, number of osteoblasts and osteoclasts, and bone microarchitecture in the low-dosage group showed no significant changes, while there were significant decrease in the serum level of calcium and bone mineral density of femur, significant increase in the number of osteoclasts at the femur and significant changes in the femoral microarchitecture in the middle- and high-dosage groups, especially in the middle-dose group. To conclude, 120 mg/(kg•d) retinoic acid via gastric lavage for 14 days can induce a stable osteoporosis model in rats.     中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Establishment of a high-precision serial colorful slice image data set of mice Tian Jun, Jia Yue, He Peng-fei, Guo Jue, Jin Rong-xiu, Guo Rui 2017, 21 (20):  3170-3175.  doi: 10.3969/j.issn.2095-4344.2017.20.010 Abstract ( 373 )   PDF (955KB) ( 368 )   Save BACKGROUND: With the extensive application of small animal imaging and imaging studies, it requires the investigators to master the cross-sectional anatomy of mice, but the anatomy of mouse nasal cavity and sinuses cannot be obtained through vivisection. OBJECTIVE: To establish a highly precise serial colorful sectional image data set of mice using digital virtual human technology. METHODS: An adult male Balb/c mouse was killed for freezing embedded using modified Kawamoto’s method and was then serially scanned at 5 μm of thickness by Leica CM3600XP cryostat microtome. The sectional images were photographed with a 24 million pixels Nikon D750 camera and stored as JPG files. All images were preliminarily registered by manually cropping the images based on the edge of block surface using software photoshop 7.0. Then, the image size, brightness and registration were corrected and adjusted, and the format of the images was unified as TIFF. The data set was imported to Amira 6.0 and was registered again with least square method. The quality of the data set was evaluated by three-dimensional reconstruction. RESULTS AND CONCLUSION: A total of 6 034 original images with a size of 87 GB were captured. After cropping, adjusting and registration, the final size was 184 G. The mouse three-dimensional reconstruction model was satisfactory. To conclude, the data set established using the method and system mentioned above is characterized by high resolution and high-fidelity, which might facilitate the further study of the precise anatomy of mice and other fundamental experiments.     中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Exercise effects on pain relief and extracellular matrix production in a rat model of intervertebral disc degeneration Luo Hai-jie, Ke Song-jian, Lin Cai-na, Wan Qing, Li Xiao, Liu Cui-cui, Ma Chao, Wu Shao-ling 2017, 21 (20):  3176-3182.  doi: 10.3969/j.issn.2095-4344.2017.20.011 Abstract ( 331 )   PDF (3366KB) ( 318 )   Save BACKGROUND: Exercise has been proved to accelerate the proliferation of intervertebral disc cells and extracellular matrix production in healthy rats. For the degenerative intervertebral disc, whether exercise also has positive effects on its cell proliferation, extracellular matrix production or pain relief remains unclear. OBJECTIVE: To investigate the effect of exercise on the extracellular matrix production in a rat model of intervertebral disc degeneration. METHODS: A rat model of intervertebral disc degeneration was prepared by Freund’s complete adjuvant injection into the intervertebral disc at L5-6 levels. Then, the model rats were allowed to have a rest for 2 weeks. All rats were then randomly divided into exercise and control groups. Rats in the exercise group were forced to run every day, while the controls allowed free activities in the cage. The behavioral tests were performed at 7, 14, 28, 42, 56 and 70 days after modeling; meanwhile, the intervertebral disc samples were collected used for alcian blue staining and immunohistochemical staining to detect the levels of proteoglycan, aggrecan and collagen type II in the intervertebral disc cells, respectively. RESULTS AND CONCLUSION: Vocalization threshold on the rat back of punctured disc was significantly decreased, while grooming and wet-dog shaking were significantly increased at 7 days after modeling compared with the baseline  (P < 0.05), suggesting that Freund’s complete adjuvant injection successfully induces disc degeneration, hyperalgesia and abnormal behaviors. Further, the vocalization threshold and wet-dog shaking in the exercise group showed significant improvement compared with the control group after 14 days of exercise (P < 0.05), while the grooming was significantly reduced until the 28th day (P < 0.01), indicating that exercise can alleviate pain caused by disc degeneration in model rats. At 21 days after modeling, the levels of proteoglycan, aggrecan and collagen type II in the nucleus pulposus and annulus fibrosus were significantly decreased compared with the baseline (P < 0.01), indicating the occurrence of disc degeneration. After 14 days of training, the levels of proteoglycan, aggrecan, and collagen type II in the nucleus pulposus and annulus fibrosus in the exercise group were significantly increased compared with the control group (P < 0.01). Moreover, after 8-week exercise, the level of proteoglycan in the nucleus pulposus and annulus fibrosus in the exercise group was increased by 4-5 times compared with the control group, and levels of aggrecan and collagen type II in the nucleus pulposus in the exercise group also was increased by 3-4 times compared with the control group. To conclude, exercise can promote extracellular matrix increased by production by increasing the levels of proteoglycan, aggrecan, and collagen type II in the degenerative intervertebral disc.     中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Expression of adenovirus-mediated nerve growth factor and myelin-associated glycoprotein double-gene in sciatic nerve injury Chen Yu, Deng Zhong-liang, Chen Shi-mou, Weng Zheng, Huang Fang 2017, 21 (20):  3183-3189.  doi: 10.3969/j.issn.2095-4344.2017.20.012 Abstract ( 263 )   PDF (1626KB) ( 327 )   Save BACKGROUND: Nerve growth factor (NGF) and myelin-associated glycoprotein (MAG) can promote the repair of peripheral nerve. However, increasing the expression of NGF and MAG in the injured peripheral nerve by transgene technology has not been reported. OBJECTIVE: To investigate the expression of NGF and MAG after adenovirus (Ad) mediated transfection in the injured sciatic nerve in rats. METHODS: Forty Sprague-Dawley rats were randomly divided into normal, model, Ad, NGF and NGF-MAG groups. Sciatic nerve injury models were established in the latter four groups by cutting and suturing the right sciatic nerve. Afterwards, the rats in each group were subject to the intramuscular injection of normal saline, normal saline, Ad (1×108 PFU), Ad-NGF (1×108 PFU), and Ad-NGF-MAG (1×108 PFU), respectively, once every 2 days, for consecutive three times. RESULTS AND CONCLUSION: The expression level of NGF in the rat sciatic nerve in the Ad and normal groups was significantly higher than that in the NGF and NGF-MAG groups (P < 0.05). Compared with the Ad, NGF and normal groups, the expression level of MAG in the NGF-MAG group was significantly increased (P < 0.05). To conclude, the Ad-mediated NGF and MAG after double gene transfection were expressed effectively in the injured sciatic nerve in rats.     中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Establishment and evaluation of a rabbit model of femoral delayed union using external fixation Zhang Zhi-tian, Wang Shu-min, Zhao Jing-xin, Zhang Meng 2017, 21 (20):  3190-3195.  doi: 10.3969/j.issn.2095-4344.2017.20.013 Abstract ( 409 )   PDF (1809KB) ( 310 )   Save BACKGROUND: Mechanisms related to bone fracture and nonunion have been clarified, but the healing time and treatment strategy of delayed union remain controversial. OBJECTIVE: To establish a reliable model of femoral delayed union using external fixation in the New Zealand white rabbit. METHODS: Seventy-two New Zealand white rabbits were randomized to three groups. After removal of 10-mm femoral middle bone cortex with internal and external periosteum and bone marrow, a uniplanar external fixator was used. There was a 1 mm gap between broken ends (group A), and the broken ends were sealed by bone wax (group B). For controls, only bone cortex was cut and then fixed to contact the broken ends. The gross, radiological and histological observations were performed to observe the bone healing at 4, 8 and 12 weeks after modeling. RESULTS AND CONCLUSION: The control group achieved bone healing at the 4th week, which was considered as the normal time of bone healing. The groups A and B achieved healing at the 12th week, which was remarkably longer than that in the control group. The rabbit femoral delayed union model showed the typical X-ray imaging and pathological features of delayed union. Our results suggest that the model is reasonable and reliable, which can provide reference for clinical research.     中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Development and application of a multifunctional impactor based on animal experiments Wang Hai-lin, Xiao He-da, Zhang Sheng, Jiang Zong-xing, Liu Wei-hui, Tang Li-jun, Dai Rui-wu 2017, 21 (20):  3196-3201.  doi: 10.3969/j.issn.2095-4344.2017.20.014 Abstract ( 346 )   PDF (987KB) ( 207 )   Save BACKGROUND: A few devices have been reported to be used for studies on trauma, but these devices are unavailable for establishing the animal models of trauma because of their limited application range. OBJECTIVE: To develop a multifunctional impact system and evaluate its application effect, thus paying ways for establishing the animal models of trauma and basic experiments. METHODS: The multifunctional impact system was designed based on the theory of energy storage device, simple multifunctional impact device and impact parameter measuring equipment, and its effectiveness and stability were detected. The rat chest and different visceral organs were subjected to the closed impact experiment using a 5 cm2 impact at the predetermined parameter of 200, 300, 400, 500 kPa, respectively. Afterwards, the rats were sacrificed for morphological observation. RESULTS AND CONCLUSION: The multifunctional impactor was successfully developed, of which the maximum impact stress could be adjusted from 0 to 200 kg and compressive and extrusion stress also could be continuously adjusted from 0 to 100 kg. The experimental results showed that the impactor made certain damage to the rat lung, liver and spleen suggesting its favorable effectiveness (P < 0.05) and repeatability (P > 0.05). These findings suggest that the impactor is easy to operate in various ways and holds good effectiveness and stability, and its impact parameters can be detected in real time. Therefore, the impactor is suitable for both establishing the animal model of trauma and basic experiments.     中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Effects of caveolin-1 on the expression of epidermal growth factor in fibroblasts co-cultured with breast cancer cells Xiao Liang, Wang Ying, Yang Xiao-jun, Yang Jun-ping 2017, 21 (20):  3202-3207.  doi: 10.3969/j.issn.2095-4344.2017.20.015 Abstract ( 317 )   PDF (889KB) ( 312 )   Save BACKGROUND: Caveolin-1, as the most important functional protein in caveolae, is involoved in a variety of cell biological processes, and is closely related to the occurrence and development of breast cancer. OBJECTIVE: To explore the effect of caveolin-1 on epidermal growth factor (EGF) in fibroblasts after co-cultured with breast cancer cells. METHODS: Caveolin-1 expression in fibroblast lines ESF-1 was interfered with siRNA, and the optimal effect was determined through QRT-PCR and western blot assay. (1) The optimal silencing model of ESF-1-Caveolin-1 SiRNA-N.2 was obtained, which was co-cultured with breast cancer cells BT474 as experimental group, single-cultured ESF-1 and ESF-1-Caveolin-1 SiRNA-N.2 as controls. The expression level of EGF in ESF-1 was detected by QRT-PCR at 24 and 48 hours of culture; the expression level of EGF in the culture medium was detected by ELISA at 48 and 72 hours of culture. (2) ESF-1-Caveolin-1 SiRNA-N.2 (experimental group) and ESF-1 (control group) were respectively co-cultured with BT474, and single-cultured BT474 as blank control group. The proliferation of BT474 was detected by cell counting-kit 8 assay after 24- and 48-hour culture. RESULTS AND CONCLUSION: The mRNA expression level of EGF in the experimental group was significantly higher than that in the other two groups (P < 0.05), and the EGF expression level in the ESF-1-Caveolin-1 SiRNA-N.2 group was significantly higher than that in the ESF-1 group (P < 0.05). The protein expression level of EGF was ranked as follows: experimental group > ESF-1-Caveolin-1 SiRNA-N.2 group > ESF-1 group (P < 0.05). The proliferation of BT474 cells was significantly increased after co-cultured with BT474 cells and ESF-1 siRNA Caveolin-1 cells, especially with BT474 cells (P < 0.05). Our findings suggest that Caveolin-1 siRNA can promote the expression of EGF in fibroblasts, especially co-cultured with breast cancer cells. Furthermore, caveolin-1 siRNA accelerates the proliferation of breast cancer cells after co-cultured with fibroblasts. Figures and Tables | References | Related Articles | Metrics

Select

Accurate determination of HLA ambiguous results based on group-specific haploid full-length sequencing Wang Song-xing, Yang Hui, He Liu-mei, Hong Wen-xu, Zou Hong-yan, Xu Yun-ping 2017, 21 (20):  3208-3215.  doi: 10.3969/j.issn.2095-4344.2017.20.016 Abstract ( 393 )   PDF (818KB) ( 294 )   Save BACKGROUND: Due to the polymorphism of HLA, a large number of ambiguities have been generated by conventional HLA typing techniques, and confirmed stereotypes of ambiguous results based on group-specific haploid full-length typing are rarely reported. OBJECTIVE: To analyze the accuracy of HLA-typing ambigulity based on group-specific haploid full-length sequencing. METHODS: The low-resolution results were used as the starting point for two ambiguous samples. Sanger sequencing (PCR-SBT) based on haploid full-length was performed after group-specific amplification. RESULTS AND CONCLUSION: One case showed a new A*02:03:01 allele, which was found a mutation in NT817 from C to T in comparison with A*11:01:01:01. The other case indicated another new C*07:02:01:01, which was found a mutation in NT879 from A to G in comparison with C*08:01:01. In conclusion, these results indicate that the group-specific haploid full-length sequencing method can be used to accurately classify HLA alleles and to discover new alleles.     中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Gap arthroplasty versus interpositional arthroplasty for temporomandibular joint ankylosis: a Meta-analysis Yishakejiang•Maimaiti, Wu Jun, Maimaitituxun•Tuerdi 2017, 21 (20):  3216-3223.  doi: 10.3969/j.issn.2095-4344.2017.20.017 Abstract ( 233 )   PDF (1025KB) ( 211 )   Save BACKGROUND: Efficacy of gap arthroplasty (GA) versus interpositional arthroplasty (IA) for temporomandibular joint ankylosis (TMJA) is rarely reported, and the treatment efficacy is still under discussion. OBJECTIVE: To systematically review the efficacy of GA versus IA in the TMJA treatment and patient’s prognosis. METHODS: A computer-based online research of the databases of WanFang, CqVip, PubMed, Ovid, Embase, Clinical Evidence, Cochrance Library combined with the manual search of relevant controlled trials that compared the resurrence postoperative maximal incision opening and/or recurrence of the two methods for TMJA published before October, 2015 was performed. According to heterogeneity results, the maximal incision opening index was analyzed by random-effect model, and the two groups were subdivided into temporomandibular myofascial flap and other material groups, and then MD and 95%CI were calculated; recurrence was analyzed through fixed effect model, and OR and 95%CI were calculated. RESULTS AND CONCLUSION: According to the inclusion and exclusion criteria, a total of 18 eligible articles involving 999 patients were enrolled, and the GA and IA were conducted in 656 and 343 cases, respectively. Meta-analysis results of the maximal incision opening were as followings: MD= -1.85, 95%CI: (-3.15, -0.55), Z=2.80, P=0.005, I2=55.4%; the temporomandibular myofascial flap subgroup: MD= -0.94, 95%CI: (-1.84, -0.04), Z=2.05, P=0.040, I2=0.0%; the other material group: MD= -3.36, 95%CI: (-6.66, -0.66), Z=2.39, P=0.017, I2=67.0%. Meta-analysis of the recurrence showed that OR=1.38, 95%CI: (0.87, 2.17), Z=1.37, P=0.170, I2=14.9%. Egger test manifested that there were no publication biases in the literatures. Sensitivity test showed that only one study influenced the total combined Meta-analysis results, and the others were steady. These results indicate that the IA can markedly increases the maximal incision opening in the TMJA treatment compared with the GA, but the two methods have the similar recurrence rate. Due to the limited quantity and quality of the included studies, the above conclusion needs to be verified by more high quality researchs. Figures and Tables | References | Related Articles | Metrics

Select

Molecular dynamics simulation: a new direction targeting physiological mechanisms of neuronal calcium sensor-1 protein Zhu Yu-zhen, Zhang Qing-wen 2017, 21 (20):  3224-3233.  doi: 10.3969/j.issn.2095-4344.2017.20.018 Abstract ( 295 )   PDF (1480KB) ( 310 )   Save BACKGROUND: Physiological functions, structural fold and unfolding of neuronal calcium sensor-1 (NCS-1) have been explored in a series of experiments, and then the possible mechanism models and key factors for remaining the structural stability are raised. But many functional models cannot be verified due to the limitations of resolution of the time and space and complex protein structure. The experimental phenomena and hypothesis or models may be tested at the atom levels by molecular dynamics, and the new structure may be predicted to provide basis for model establishment and functional mechanisms. OBJECTIVE: To overview the research process of physiological functions and mechanisms of NCS-1 using the experimental method and molecular dynamics simulations, thereby providing basis for future research. METHODS: PubMed database was retrieved for the literatures addressing NCS-1 using the English subject term “Neuronal Calcium Sensor-1 or Neuronal Calcium Sensor1 or Neuronal Calcium Sensor 1 or NCS-1”. Finally, 72 articles were included in result analysis based on the inclusion and exclusion criteria. RESULTS AND CONCLUSION: The theoretical models of NCS-1 in secretion regulation, dopamine D2 receptor regulation, adenosine A2A receptor regulation in hepatocytes and Ca2+ regulation in myocardial cytoplasm and nuclei with different stimuli are put forward. The key factors to remaining structural stability are analyzed and summarized by modular dynamics simulation in view of structure. It is recommended to combine these two methods in order to deeply understand the protein functional mechanisms, thereby pushing the in-depth study.     中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Cell therapy is the most promising technique for degenerative intervertebral disc repair Wang Yan-chao, Xi Zhi-peng, Xie Lin 2017, 21 (20):  3234-3240.  doi: 10.3969/j.issn.2095-4344.2017.20.019 Abstract ( 224 )   PDF (762KB) ( 288 )   Save BACKGROUND: Current treatmemt strategy cannot efficiently relieve or reverse the disk degeneration, and neither surgical treatment nor nonsurgical treatment has gained satisfactory long-term effect. Therefore, more and more researches have focused on the cell therapy. OBJECTIVE: To explore the present states and application prospect of cell therapy, especially stem cells for degenerative intervertebral disc repair. METHODS: A computer-based search for related articles in PubMed database published between January 2011 and January 2016 using the English keywords of “stem cell, intervertebral disk degeneration”. Literatures addressing cell therapy for degenerative intervertebral disc repair were selected, and the articles published lately or original researches in authoritative journals were preferred. RESULTS AND CONCLUSION: A total of 205 articles were selected firstly, and 50 eligible articles were enrolled finally in accordance with the inclusion criteria. The mixtures of stem cells and carrier are injected into the degenerative intervertebral disk, to repair or displace abnormal cells. This strategy has been accepted by many researchers, and considered as a promising treatment. However, there is little evidence about the safety of clinical treatment with cell therapy, which still needs to be explored in depth.     中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Roles of cytokines and three-dimensional printing technology in skeletal muscle repair Wang Kang-kang 2017, 21 (20):  3241-3247.  doi: 10.3969/j.issn.2095-4344.2017.20.020 Abstract ( 296 )   PDF (749KB) ( 204 )   Save BACKGROUND: The regeneration ability of skeletal muscle is limited, and cytokines play an important role in skeletal muscle repair. A variety of cells and regulatory molecules are involved in skeletal muscle repair. Three-dimensional (3D) printing technique is an advanced technology used to construct an in vitro multicellular system with biological activity and has aroused widely attention in skeletal muscle repair. OBJECTIVE: To investigate the role of proinflammatory cytokines and cell growth factors in skeletal muscle repair, and to summarize the advantages and application of 3D printing technology in skeletal muscle repair. METHODS: A computer-based retrieval of PubMed and WanFang databases was conducted for the articles addressing the roles of cytokines and application of 3D printing technique in skeletal muscle injury published from 2002 to 2015. Totally 146 articles were searched firstly, and finally 45 eligible articles were enrolled in accordance with the inclusion and exclusion criteria. RESULTS AND CONCLUSION: Proinflammatory cytokines, such as tumor necrosis factor-α, interleukin-1β, interleukin-6, and anti-inflammatory cytokines, such as interleukin-10, interleukin-4, play a critical role in skeletal muscle repair. Cell growth factors such as insulin-like growth factor, fibroblast growth factor, transforming growth factor can effectively promote the proliferation of myoblasts and cell differentiation into myotubes, further promoting the proliferation of skeleton satellite cells. Additionally, these cytokines act mutually, which is of great significance for skeletal muscle repair. 3D printing technology can quickly and accurately reproduce and reconstruct the complex structure of target tissues or organs, so it has been extensively applied in toxicology research, medical testing and basic experiments. The role of 3D printing technology in skeletal muscle repair will be a hotspot in the future.     中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

MG53 protein protects against multiorgan ischemia/reperfusion injury: present and future Liu Teng-fei, Zhou Jian-kang, Huang Tuan-jie, Xing Qu, Cheng Kang, Li Peng, Li Dong-peng, Yang Bo, Ma Shan-shan, Guan Fang-xia 2017, 21 (20):  3248-3254.  doi: 10.3969/j.issn.2095-4344.2017.20.021 Abstract ( 349 )   PDF (1035KB) ( 236 )   Save BACKGROUND: In recent years, with the progress of shock therapy as well as the establishment and promoted application of arterial bypass grafting, thrombolytic therapy, percutaneous transluminal coronary angioplasty, extracorporeal circulation on cardiac surgery, cardiopulmonary resuscitation, limb replantation, and organ transplantation, blood reperfusion in multiple organs after ischemia has been achieved. However, the organs which undergo a period of ischemia appear to have the performance of damage aggravation. OBJECTIVE: To summarize the research progress of MG53 protein in protecting five organs from ischemia/reperfusion injury, thereby providing reference for further in-depth study. METHODS: A computer-based online search of PubMed, Duxiu Knowledge Search and CNKI databases was performed for relevant literatures puldished between 1986 and 2016. The key words were “MG53, TRIM, Mitsugumin53, ischemic, reperfusion, preconditioning, postconditioning, RISK, membrane damage, Connexin43, KChIP2” in English and “MG53, ischemia/reperfusion” in Chinese. Finally 61 eligible articles were reviewed in accordance with the inclusion and exclusion criteria. RESULTS AND CONCLUSION: As a muscle-specific TRIM family protein, endogenous MG53 is involved in the repair of muscle cytomembrane damage, and the protective effects of ischemic preconditioning and postconditioning. Exogenous recombinant human MG 53 protein not only repairs membrane damage of various muscles and non-muscle cells, but also protects the myocardium, skeletal muscle, brain, lung and kidney from ischemia/reperfusion injury.   中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Pathogenesis and treatment of osteoarthritis: the latest progress Zheng Xiao-fen 2017, 21 (20):  3255-3262.  doi: 10.3969/j.issn.2095-4344.2017.20.022 Abstract ( 525 )   PDF (1091KB) ( 511 )   Save BACKGROUND: The etiology of osteoarthritis is not yet clear. There are a large number of studies on the pathogenesis of osteoarthritis, such as cartilage degeneration, subchondral bone degeneration, joint mechanical changes, and nerve conduction abnormality. OBJECTIVE: To explore the latest advances in the pathogenesis and treatment methods of osteoarthritis at home and abroad. METHODS: The literatures concerning osteoarthritis at home and abroad were searched using the keywords of “osteoarthritis, mechanism, therapy” in English and Chinese, respectively. Finally, 46 eligible literatures were enrolled for overview. RESULTS AND CONCLUSION: The incidence of osteoarthritis is closely related to the degeneration of articular cartilage and subchondral bone, changes in joint mechanics and arthralgia. Superficial zone tissues of articular cartilage vary with age increasing, which is easy to cause osteoarthritis. The treatment should lay emphasis on prevention so as to reduce the risk of osteoarthritis and delay its progression, but not on the combination of non-drug and drug-based treatment. Notably, obesity is the most important risk factor for osteoarthritis. Non-steroid anti-inflammatory drug is the first choice for the treatment of knee osteoarthritis according to Guidelines for the Prevention and Treatment of Osteoarthritis. Fish oil cannot alleviate the progression of osteoarthritis. Surgical treatment is not recommended in the early osteoarthritis, osteoarthritis will not be cured completely through arthroscopic operation, and arthroplasty is recommended for those severe patients.   中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Hypothermia regulates the internal environment following spinal cord injury: the underlying mechanisms Zhu Shuang-long, Chen Xu-yi, Xu Yun-qiang, Zhang Sai 2017, 21 (20):  3263-3268.  doi: 10.3969/j.issn.2095-4344.2017.20.023 Abstract ( 227 )   PDF (876KB) ( 307 )   Save BACKGROUND: Spinal cord injury is a damage to the spinal cord with a high morbidity that can be divided into primary and secondary injury. Secondary injury does more harm to the body than primary injury, which can be regulated and improved through proper interventions. In addition to the drugs, surgical decompression and other traditional treatments, hypothermia is an important physical intervention that has been shown to regulate secondary injury following spinal cord injury, and hold neuroprotective effect. OBJECTIVE: To introduce different hypothermia treatments for spinal cord injury and the effect on the disordered environment after spinal cord injury, as well as summarize the latest progression. METHODS: A computer-based search of CNKI and PubMed databases was conducted for the articles addressing the application of hypothermia in spinal cord injury published from January 2001 to June 2016, using the keywords of “therapeutic hypothermia or low temperature, spinal cord injury” in Chinese and English, respectively. RESULTS AND CONCLUSION: Hypothermia is divided into systematic and local hypothermia, and the former is simple and convenient, but it may lead to complications. Local hypothermia can quickly reach the target temperature, to make deep hypothermia at injury site and stablize the core temperature in the body, but it is invasive, and the necessary time of locating and maintaining effective temperature is a challenge. In general, hypothermia can improve the disordered microenvironment after spinal cord injury, reduce inflammatory infiltration, regulate the expression of relative genes and proteins, and promote the proliferation and differentiation of endogenous nerve cells. There are basic and clinical studies on hypothermia neuroprotection against spinal cord injury from various aspects; thereafter, hypothermia is a promising treatment strategy for spinal cord injury.   中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Hyperbaric oxygen therapy and comprehensive orthopedic treatment for incomplete traumatic spinal cord injury on the Qinghai-Tibet Plateau: study protocol for an open-label randomized controlled clinical trial Sun Qing, Bao Jian-feng, An Yu-lan, Lei Hui, Ma Jun 2017, 21 (20):  3269-3274.  doi: 10.3969/j.issn.2095-4344.2017.20.024 Abstract ( 451 )   PDF (904KB) ( 214 )   Save BACKGROUND: Apoptosis secondary to ischemia and hypoxia is the main cause of spinal cord dysfunction. Because of the decrease in atmospheric pressure, patients living on the Qinghai-Tibet Plateau are in a hypoxic environment, which is very unfavorable for the recovery of spinal cord injury. Hyperbaric oxygen therapy can improve the postoperative function of patients with incomplete spinal cord injury, and its effect is better on the plateau than at normal altitudes. OBJECTIVE: To observe the effect of hyperbaric oxygen therapy on traumatic spinal cord injury in patients living on the Qinghai–Tibet Plateau. METHODS: This prospective, open-label, randomized controlled clinical trial was performed at the Department of Spine Surgery, Affiliated Hospital of Qinghai University, China. In total, 164 patients with incomplete traumatic spinal cord injury were equally and randomly assigned to a control group and a hyperbaric oxygen therapy group. Patients in the control group were treated with pedicle screw fixation and decompressive laminectomy. In addition to the surgical treatment performed in the control group, patients in the hyperbaric oxygen group underwent hyperbaric oxygen therapy at      0.2 MPa once a day for four treatment courses. Ten treatment sessions constituted one course, and each course was separated by a 5- to 7-day rest interval. The primary outcome was the modified Barthel index to assess activities of daily living. The secondary outcomes were the American Spinal Injury Association (ASIA) impairment scale grade, sensory score, and motor score. The study protocol was approved by the Ethics Committee of the Affiliated Hospital of Qinghai University, China (Approval number: QHC011K). Written informed consent was provided by a relative or legal representative of each patient after they had indicated that they fully understood the treatment plan. RESULTS AND CONCLUSION: The partial results demonstrated that after four treatment courses (55-61 days), the modified Barthel index and ASIA tactile, pain, and motor scores were higher in the hyperbaric oxygen group than in the control group. The ASIA grades were significantly different between the hyperbaric oxygen group and control group. The proportion of patients with ASIA grades D and E was higher in the hyperbaric oxygen group than in the control group. In this trial, we aim to determine the efficacy of hyperbaric oxygen therapy on the treatment of incomplete traumatic spinal cord injury in patients living on the plateau and to provide clinical evidence for treating incomplete traumatic spinal cord injury in these patients.   中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics

Select

Risk factors for new-onset diabetes mellitus after kidney transplantation: a retrospective study Yang Jin, Fang Fang, Yang Fu 2017, 21 (20):  3275-3280.  doi: 10.3969/j.issn.2095-4344.2017.20.025 Abstract ( 308 )   PDF (792KB) ( 381 )   Save BACKGROUND: Effect of metabolic disorders after kidney transplantation on prognosis, especially new-onset diabetes after transplantation (NODAT), has aroused concerns. OBJECTIVE: To investigate the risk factors for NODAT in renal transplant recipients, thus providing reference for identifying high-risk patients and taking active prevention measures. METHODS: Clinical data of 365 patients undergoing allograft renal transplantation were analyzed retrospectively. According to whether NODAT occurred or not, all the patients were assigned to NODAT (n=71) and non-NODAT (n=294) groups. The independent risk factors for NODAT were determined using univariate  and non-conditional multivariate logistic regression analysis. RESULTS AND CONCLUSION: The cumulative incidence of NODAT in renal transplant recipients within year postoperatively was 19.45%. Non-conditional multivariate logistic regression analysis showed that age ≥ 45 years old, body mass index ≥ 25 kg/m2, family history of diabetes mellitus and acute rejection were associated with NODAT in renal transplant recipients. To conclude, the age, family history of diabetes mellitus, high body mass index and acute rejection can increase the incidence of NODAT in renal transplant recipients.   中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程 Figures and Tables | References | Related Articles | Metrics