Biocompatibility of rabbit annulus fibrosus-derived stem cells with genipin cross-linked decellularized annulus fibrosus matrix/chitosan hydrogels

doi:10.3969/j.issn.2095-4344.2016.21.015

Abstract

Abstract:

BACKGROUND: To improve the mechanical properties and uncontrollability of degradation of decellularized matrix, we manufactured genipin cross-linked decellularized annulus fibrosus matrix/chitosan hydrogels as annulus fibrosus tissue-engineered scaffold.

OBJECTIVE: To observe the biocompatibility of annulus fibrosus-derived stem cells with genipin cross-linked decellularized annulus fibrosus matrix/chitosan hydrogels, and the degradation of hydrogels in vivo.

METHODS: Genipin cross-linked decellularized annulus fibrosus matrix/chitosan hydrogel scaffolds were prepared, and the internal structure and hydrophilic property of hydrogels was detected by scanning electron microscopy and contact angle meter, respectively. Afterwards, passage 1 rabbit annulus fibrosus-derived stem cells were seeded on the scaffolds for 3 days, cell morphology was observed by inverted immunofluorescence microscopy and scanning electron microscopy after cytoskeleton staining, and additionally, curve of cell growth was obtained. In the meanwhile, the scafflods were transplanted into the intermuscular space of New Zealand white rabbits, and the degradation and inflammatory reaction of the hydrogels were observed after 4 weeks.

RESULTS AND CONCLUSION: The prepared hydrogel scaffold showed porous and network structure and the contact angle was (39.94±1.61)°; annulus fibrosus-derived stem cells adhered well on the scaffold surface with a faster growth rate. Furthermore, at 4 weeks after transplantation, the hydrogel in vivo was degraded, and inflammatory cells appeared. These findings indicate that genipin cross-linked decellularized annulus fibrosus matrix/chitosan hydrogels have good biocompatibility.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Chitosan, Hydrogel, Stem Cells, Tissue Engineering

CLC Number:

R318

Liu Chen, Zhao Quan-lai, Wang Ling-ting, Wang Hong, Liu Ping, Li Bin, Xu Hong-guang. Biocompatibility of rabbit annulus fibrosus-derived stem cells with genipin cross-linked decellularized annulus fibrosus matrix/chitosan hydrogels[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(21): 3143-3149.

Figures/Tables 6

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