Biocompatibility and security of calcium sulfate bone substitutes

doi:10.3969/j.issn.2095-4344.2016.16.005

Abstract

Abstract:

BACKGROUND: It is a great potential study that calcium sulfate product loaded with antibiotics is developed, but this product is not systematically studied and its biocompatibility and security need to be further studied.

OBJECTIVE: To evaluate the biocompatibility and safety of vancomycin- or gentamicin-loaded calcium sulfate bone.

METHODS: (1) Hemolysis test: vancomycin-loaded, gentamicin-loaded calcium sulfate extracts, double distilled water and saline were added into rabbit anticoagulant blood samples. (2) Micronucleus test: vancomycin-loaded and gentamicin-loaded calcium sulfate extracts, cyclophosphamide and normal saline solution were intraperitoneally injected to mice, respectively. (3) Acute toxicity test: vancomycin-loaded and gentamicin-loaded calcium sulfate extracts, and normal saline solution were intraperitoneally injected to mice, respectively. (4) Pyrogen test: the mice were injected vancomycin-loaded and gentamicin-loaded calcium sulfate extracts via the ear vein. (5) Intradermal stimulation test: vancomycin-loaded and gentamicin-loaded calcium sulfate extracts were respectively injected into the unilateral spine of rabbits, respectively. (6) Intramuscular implantation test: vancomycin-loaded and gentamicin-loaded calcium sulfate extracts were respectively injected to the dorsal muscle of rabbits. (7) Intraosseous implantation test: vancomycin-loaded and gentamicin-loaded calcium sulfate were implanted into the necrotic femoral bone of rabbits.

RESULTS AND CONCLUSION: Both vancomycin-loaded and gentamicin-loaded calcium sulfate products, which have no hemolytic reaction, genetic toxicity, acute toxicity, pyrogen reaction and skin irritation, are considered to have good biocompatibility and safety.
中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Calcium Sulfate, Materials Testing, Vancomycin, Tissue Engineering

Qiu Yi-yan, Chen Yang, Zhou Wen-yu, Xia Xiao-long, Yang Xin-jian. Biocompatibility and security of calcium sulfate bone substitutes[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(16): 2317-2323.

Figures/Tables 5

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