Surgical biomaterials to repair the ruptured annulus fibrosus due to intervertebral disc degeneration

doi:10.3969/j.issn.2095-4344.2016.16.006

Abstract

Abstract:

BACKGROUND: In recent years, animal models of lumbar disc degeneration have been popularized to explore the effect of tissue engineering, cell engineering and genetic engineering technologies on intervertebral disc degeneration.

OBJECTIVE: To investigate the influence of biological patches on rabbit anulus fibrosus repair.

METHODS: L_3-4, L_4-5, L_5-6 segments from rabbits were randomly divided into normal control group (the intervertebral disc was exposed correspondingly), control group (the annulus fibrosus was only cut with a surgical scalpel) and experimental group (the annulus fibrosus was cut and sutured with the surgical biomaterial). Six rabbits were selected randomly to take the lumbar X-ray and MRI preoperatively and 1, 2, 4, 8, 12 weeks postoperatively; one rabbit was chosen preoperatively and three rabbits selected respectively at 1, 2, 4, 8, 12 weeks postoperatively to execute hematoxylin-eosin and type II collagen immunohistochemistry.

RESULTS AND CONCLUSION:At postoperative 1, 2 and 4 weeks, the disc height index decreased significantly in the control and experimental groups. MRI and histopathological examination showed that the T2WI signal intensity and hematoxylin-eosin grading were both increased significantly in the control and experimental groups at 2 weeks after surgery (P < 0.05). With time, the number of nucleus pulposus cells gradually reduced in the control and experimental groups. Annulus fibrosus defects were filled with granulation and fibrous tissues, and the biofilm was tightly fused with the annulus fibrosus. Findings from the type II collagen immunohistochemical staining showed that the histological staining of the nucleus pulposus was gradually changed from positive to negative in the experimental and control groups. Therefore, cutting the annulus fibrosus can lead to severe disc degeneration at early period, and surgical biomaterials can be integrated with the annulus fibrosus well to seal annulus fibrosus defects and further prevent nucleus pulposus protrusion. However, this approach cannot restrain the continuous process of disc degeneration.
中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Tissue Engineering, Intervertebral Disk, Biocompatible Materials

Zhu Xu, Ma Yuan, Meng Xiang-yu, Tian Hui-zhong. Surgical biomaterials to repair the ruptured annulus fibrosus due to intervertebral disc degeneration[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(16): 2324-2332.

Figures/Tables 8

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