Absorbable bio-glass injection in osteoporosis mice: a support for the osteoporotic vertebral body and mechanism of osteogenic induction

doi:10.3969/j.issn.2095-4344.2016.16.004

Abstract

Abstract:

BACKGROUND: Absorbable bio-glass injection composed by bio-glass and calcium phosphate bone cement can be tightly combined with bone tissue by a strong chemical bond to improve the stability of the bone-implant interface.

OBJECTIVE: To investigate the effect of absorbable bio-glass injection to support the vertebral body of osteoporosis mice and its mechanism of osteogenic induction.

METHODS: Osteoporosis models were prepared in 30 female Sprague-Dawley rats undergoing bilateral ovariectomy. After modeling, model rats were randomly divided into three groups, and given polymethylmethacrylate, injectable calcium phosphate bone cement and absorbable bio-glass injection into L_3-5 vertebral bone defects, respectively. L_3-5 segments were removed at 12 weeks after implantation to detect the biomechanical and degradation properties, levels of calcium and phosphate, alkaline phosphatase activity, bone mineral density, levels of bone morphogenetic protein 2 and transforming growth factor β, as well as histological observation.

RESULTS AND CONCLUSION: In the absorbable bio-glass injection group, the degradation properties, compressive strength, surface hydroxyapatite deposition amount and bone mineral density were significantly higher than those in the other two groups(P < 0.05); trabecular bone relative volume, thickness and number were significant higher than those in the other two groups (P < 0.05); serum levels of calcium, alkaline phosphatase, bone morphogenetic protein 2 and transforming growth factor β were significantly higher than those in the other two groups(P < 0.05); but the level of serum phosphate was lower than that in the other two groups(P < 0.05). These results show that the absorbable bio-glass injection can enhance the support for the osteoporotic vertebral body and induce osteogenesis, probably by increasing bone morphogenetic protein 2 and transforming growth factor β levels.
中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Osteoporosis, Methylmethacrylates, Calcium Phosphates, Tissue Engineering

Bian Jing, Gong Tai-fang, Chen Wen, Zheng Hong-mei. Absorbable bio-glass injection in osteoporosis mice: a support for the osteoporotic vertebral body and mechanism of osteogenic induction[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(16): 2310-2316.

Figures/Tables 5

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