In vivo animal study on osteal histocompatibility of carbon fiber-reinforced nano-hydroxyapatite/polyamide 66 composites

doi:10.3969/j.issn.2095-4344.2015.16.012

Abstract

Abstract:

BACKGROUND: Compared with hydroxyapatite materials and other nano-hydroxyapatite composites, carbon fiber-reinforced nano-hydroxyapatite/polyamide 66 composites have been significantly improved in the mechanical strength, toughness, elastic modulus and other aspects. It can be used for repairing bone defects of loading parts.
OBJECTIVE: To investigate the biocompatibility of carbon fiber-reinforced nano-hydroxyapatite/polyamide 66 composites in bone tissues.
METHODS: Eight Bama mini pigs were taken to establish models of thoracic vertebral defects and implanted with carbon fiber-reinforced nano-hydroxyapatite/polyamide 66 composites. At 8, 16 and 24 weeks after implantation,
the animals were sacrificed, respectively, for bone mineral density detection and hematoxylin-eosin staining. Blood samples for kidney and liver function tests were taken before and 1 and 8 weeks after implantation.
RESULTS AND CONCLUSION: Hematoxylin-eosin staining of bone samples showed that the materials could bond with the bone defect interface without rejection, and could induce osteogenesis of chondrocytes. At 8 weeks after surgery, the broken ends of cancellous bone closed and the composite material was wrapped by granulation tissues. At 16 weeks after surgery, granulation tissues were organized and new bone developed directly from fibroblast cells. The new bone tissues were nearly fused with the end of cancellous bone. At 24 weeks after surgery, new bone tissue became mature lamellar bone, and the end of cancellous bone was connected tightly with the composite material. Bone mineral density of the implanted vertebra showed an increase trend at 8, 16 and 24 weeks after implantation. Over time, the bone mass was increased. The liver and kidney function tests showed that there was no significant difference before and after implantation. It is preliminarily believed that the carbon fiber-reinforced nano-hydroxyapatite/polyamide 66 composite has excellent histocompatibility and bioactivity without hepatic toxicity and nephritic toxicity.

Key words: Hydroxyapatites, Histocompatibility, Nanoparticles, Bone and Bones, Animal Experimentation

CLC Number:

R318

Lu Ming, Zhang Xue-song, Xu Hui, Hu Wen-hao, Yang Xiao-qing . In vivo animal study on osteal histocompatibility of carbon fiber-reinforced nano-hydroxyapatite/polyamide 66 composites[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(16): 2523-2528.

Figures/Tables 2

References

[1] 廖世波,赖琛,张志雄,等.骨修复复合材料及其相关问题的研究进展[J].中国生物医学工程学报,2012,31(5):755-761.
[2] Mills LA, Simpson AH. In vivo models of bone repair. J Bone Joint Surg Br. 2012;94(7):865-874.
[3] Kolk A, Handschel J, Drescher W, et al. Current trends and future perspectives of bone substitute materials-from space holders to innovative biomaterials. J Craniomaxillofac Surg. 2012;40(8):706-718.
[4] Heo SJ, Kim SE, Wei J, et al. Fabrication and characterization of novel nano-and micro-HA/PCL composite scaffolds using a modified rapid prototyping process. J Biomed Mater Res A. 2009;89(1):108-116.
[5] 李鸿.多孔纳米寒基磷灰石/聚酸胺复合骨修复材料的研究[D].成都:四川大学,2007.
[6] Wang X, Li Y, Wei J, et al. Development of biomimetic nano-hydroxyapatite/poly (hexamethylene adipamide) composites. Biomaterials. 2002;23(24):4787-4791.
[7] Thian ES, Huang J, Ahmad Z, et al. Influence of nanohydroxyapatite patterns deposited by electrohydrodynamic spraying on osteoblast response. J Biomed Mater Res A. 2008;85(1):188-194.
[8] Abd EI-Fatah H, Helmy Y, EI-Kholy B, et al. In vivo animal histomorphometric study for evaluating biocompatibility and osteointegration of nano-hydroxyapatite as biomaterials in tissue engineering. J Egypat Natl Canc lust. 2010;22(4): 241-250.
[9] Zhang X, Zhang Y, Zhang X, et al. Mechanical properties and cytocompatibility of carbon fibre reinforced nano-hydroxyapatite/polyamide66 ternary biocomposite. J Mech Behav Biomed Mater. 2015;42:267-273.
[10] Zhang X, Li Y, Lv G, et al. Mechanical properties and cytocompatibility of carbon fibre reinforced nano-hydroxyapatite/polyamide66 ternary biocomposite. Polym Degrad Stabil. 2006;91(5):1202-1207.
[11] Kanyama M, Hashimoto T, Shigenobu K, et al. Pitfall of anterior cervical fusion using titanium mesh and local autograft. J Spine Disord Tech. 2003;16(6):513-518.
[12] Das K, Couldwell WT, Sava G, et al. Use of cylindrical titanium mesh and locking plates in anterior cervical fusion. J Neurosurg. 2001;94(1 Suppl):174-178.
[13] Wang L, Song Y, Pei F, et al. Application of nano-hydroxyapatite/ polyamide 66 cage in reconstruction of spinal stability after resection of spinal tumor. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2011;25(8):941-945.
[14] Nandi SK, Roy S, Mukherjee P, et al. Orthopaedic applications of bone graft & graft substitutes: a review. Indian J Med Res. 2010;132:15-30.
[15] 王岩松,刘丹平,张元和,等.纳米羟基磷灰石/聚酰胺66骨填充材料与自体骨修复良性骨肿瘤植入后骨缺损的对比研究[J].军医进修学院学报,2011,23(4):373-374.
[16] 杨曦,宋跃明,孔清泉,等.纳米羟基磷灰石/聚酰胺66椎间融合器植骨融合治疗下腰椎退变性疾病的近期疗效[J].中国修复重建外科杂志,2012,26(12):1420-1424.
[17] 陈豪.比较纳米羟基磷灰石/聚酰胺66人工椎体与自体髂骨在颈椎前路减压融合中的应用[J].中国组织工程研究,2011,15(12): 2195-2198.
[18] 修鹏,刘利岷,宋跃明,等.纳米羟基磷灰石/聚酰胺66椎体支撑体在脊髓型颈椎病前路手术重建中的应用[J].中国骨与关节外科,2009, 2(5):23-26.
[19] Zhang Y, Quan ZX, Zhao ZH, et al. Evaluation of anterior cervical reconstruction with titanium mesh cages versus nano- hydroxyapatite/polyamide66 cages after 1- or 2-level corpectomy for multilevel cervical spondylotic myelopathy: a retrospective study of 117 patients. PLoS One. 2014;9(5): e96265.
[20] Zhang ZH, Jiang DM, Ou YS, et al. A hollow cylindrical nano-hydroxyapatite/polyamide composite strut for cervical reconstruction after cervical corpectomy. J Clin Neurosci. 2012;19(4):536-540.
[21] Yang X, Song Y, Liu L, et al. Anterior reconstruction with nanohydroxyapatite/polyamide-66 cage after thoracic and lumbar corpectomy. Orthopedics. 2012;35(1):66-73.
[22] Yang X, Chen Q, Song Y, et al. Comparison of anterior cervical fusion by titanium mesh cage versus nano-hydroxyapatite/polyamide cage following single-level corpectomy. Int Orthop. 2013;37(12):2421-2427.
[23] 朱雪松,杨惠林,张志明,等.椎体成形术骨缺损动物模型的制备和评价[J].苏州大学学报(医学版),2005,(4):599-601.
[24] 杨晓波,裴福兴,严永刚,等.聚氨基酸复合纳米羟基磷灰石的体内生物相容性实验研究[J].中国矫形外科杂志,2010,(21): 1809-1813.
[25] Yoshikawa H, Myoui A. Bone tissue engineering with porous hydroxyapatite ceramics. J Artif Organs. 2005;8(3): 131-136.
[26] 桑裴铭.医用纳米羟基磷灰石/聚酰胺66复合生物材料研究现状及进展[J].医学综述,2012;18(13):2072-2074.
[27] Mastrogiacomo M, Muraglia A, Komlev V, et al. Tissue engineering of bone: search for a better scaffold. Orthod Craniofac Res. 2005;8(4):277-284.
[28] 强小虎,王彦平.聚磷酸钙纤维/纳米羟基磷灰石/聚乳酸复合材料的降解行为[J].中国组织工程研究与临床康复,2008,(27): 5275-5278.
[29] 卢晓英,王秀红,屈树新,等.纳米羟基磷灰石/壳聚糖杂化材料的制备[J].无机材料学报,2008,(2):332-336.
[30] 杨辉,张林,张宏,等.丝素蛋白/羟基磷灰石复合材料的制备及性能表征[J].复合材料学报,2007,(3):141-146.
[31] Wei J, Li YB. Tissue engineering scaffold material of nano-apatitecrystals and polyamide composite. Eur Poly J. 2004;40(3):509-515.
[32] Yang B, Ou Y, Jiang D, et al. Early clinical effect of intervertebral fusion of lumbar degenerative disease using nano-hydroxyapatite/polyamide 66 intervertebral fusion cage. Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2014;31(5): 1102-1106.
[33] Sang PM, Zhang M, Chen BH, et al. Radiological study on the n-HA/PA66 cage used in the transforaminal lumbar interbody fusion. Zhongguo Gu Shang. 2014;27(8):654-657.
[34] Yang X, Song YM, Liu LM, et al. Anterior decompression and fusion with n-HA/PA66 cage for the treatment of lower cervical fracture and dislocation. Zhongguo Gu Shang. 2014;27(2): 92-96.
[35] Yang X, Liu L, Song Y, et al. Outcome of single level anterior cervical discectomy and fusion using nano-hydroxyapatite/ polyamide-66 cage. Indian J Orthop. 2014;48(2):152-157.
[36] Yang X, Chen Q, Liu L, et al. Comparison of anterior cervical fusion by titanium mesh cage versus nano-hydroxyapatite/polyamide cage following single-level corpectomy. Int Orthop. 2013;37(12):2421-2427.
[37] Ryu SY, Kwak SY. Role of electrical conductivity of spinning solution on enhancement of electrospinnability of polyamide 6,6 nanofibers. J Nanosci Nanotechnol. 2013;13(6): 4193-4202.
[38] Su B, Peng X, Jiang D, et al. In vitro and in vivo evaluations of nano-hydroxyapatite/polyamide 66/glass fibre (n-HA/PA66/GF) as a novel bioactive bone screw. PLoS One. 2013;8(7):e68342.
[39] Liu M, Zhang Q, Zhou L, et al. Research on the microstructure of antibacterial nanocomposite membrane and it's biocompatibility as a guided bone regeneration membrane. Hua Xi Kou Qiang Yi Xue Za Zhi. 2013;31(2):127-130, 135.
[40] James D, Varga A, Jesperson GD, et al. Identification and complete genome analysis of a virus variant or putative new foveavirus associated with apple green crinkle disease. Arch Virol. 2013;158(9):1877-1887.
[41] Qiao B, Li J, Zhu Q, et al. Bone plate composed of a ternary nano-hydroxyapatite/polyamide 66/glass fiber composite: biomechanical properties and biocompatibility. Int J Nanomedicine. 2014;9:1423-1432.
[42] Xiong Y, Ren C, Zhang B, et al. Analyzing the behavior of a porous nano-hydroxyapatite/polyamide 66 (n-HA/PA66) composite for healing of bone defects.Int J Nanomedicine. 2014;9:485-494.
[43] Qian X, Yuan F, Zhimin Z, et al. Dynamic perfusion bioreactor system for 3D culture of rat bone marrow mesenchymal stem cells on nanohydroxyapatite/polyamide 66 scaffold in vitro.J Biomed Mater Res B Appl Biomater. 2013;101(6):893-901.
[44] Zhang SL, Zhou Y, Duan H, et al. Repairing bone defect with nano-hydroxyapatite and polyamide 66 composite after giant cell tumor operations. Sichuan Da Xue Xue Bao Yi Xue Ban. 2012;43(3):373-377.
[45] Lu M, Jiang D, Quan Z, et al. Experimental research of antibacterial property and release characteristics of Titania and Ag containing nano-hydroxyapatite/polyamide 66 composite bone filling material in vitro. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2010;24(6):691-695.
[46] Xu Q, Lu H, Zhang J, et al. Tissue engineering scaffold material of porous nanohydroxyapatite/polyamide 66. Int J Nanomedicine. 2010;5:331-335.
[47] Liang X, Jiang D, Ni W. Clinical observation on nano-hydroxyapatite and polyamide 66 composite in repairing bone defect due to benign bone tumor. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2007;21(8):785-788.
[48] Zheng Q, Zhou LW, Wei SC, et al. Experimental study on the reconstruction of mandibular defects with a new bioactive artificial bone nano-hydroxyapatite/polyamide-66 in dogs. Zhonghua Kou Qiang Yi Xue Za Zhi. 2004;39(1):60-62.
[49] Wei S, Li Y, Zheng Q, et al. Study on injectable bioactive bone repairing material of nano-hydroxyapatite and polyamide-66 composite. Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2003; 20(4):590-593