Chinese Journal of Tissue Engineering Research ›› 2026, Vol. 30 ›› Issue (24): 6207-6213.doi: 10.12307/2026.211

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Differences in angiogenesis and osteogenic effects between autogenous bone and mixed bone in guided bone regeneration

Yang Ning1, 2, Han Zekui1, 2, Wang Xinyu1, 2, Huang Yiping1, 2, Han Jiaqi1, 2, Wang Yu1, 2, Duan Feng1, 2   

  1. 1Key Laboratory of Oral Biomedical Materials and Clinical Applications of Heilongjiang Province, Jiamusi 154002, Heilongjiang Province, China; 2School of Stomatology, Jiamusi University, Jiamusi 154002, Heilongjiang Province, China 
  • Received:2025-06-20 Revised:2025-09-20 Online:2026-08-28 Published:2026-01-29
  • Contact: Duan Feng, MS, Chief physician, Key Laboratory of Oral Biomedical Materials and Clinical Applications of Heilongjiang Province, Jiamusi 154002, Heilongjiang Province, China; School of Stomatology, Jiamusi University, Jiamusi 154002, Heilongjiang Province, China Co-corresponding author: Han Zekui, MS, Attending physician, Key Laboratory of Oral Biomedical Materials and Clinical Applications of Heilongjiang Province, Jiamusi 154002, Heilongjiang Province, China; School of Stomatology, Jiamusi University, Jiamusi 154002, Heilongjiang Province, China
  • About author:Yang Ning, MS, Physician, Key Laboratory of Oral Biomedical Materials and Clinical Applications of Heilongjiang Province, Jiamusi 154002, Heilongjiang Province, China; School of Stomatology, Jiamusi University, Jiamusi 154002, Heilongjiang Province, China
  • Supported by:
    Key Research and Development Program of Heilongjiang Province, No. SC2024ZX12C0092 (to WXY)

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Abstract: BACKGROUND: Angiogenesis is a key factor in the success of guided bone regeneration, but the impact of different bone graft materials on angiogenesis and osteogenic effects remains unclear.
OBJECTIVE: To compare the angiogenesis and osteogenic effects of autologous bone powder and the mixture of autologous bone powder and artificial bone powder during guided bone regeneration.
METHODS: Preoperative scanning of the jawbone data of New Zealand white rabbits using cone beam CT was performed to extract a rabbit jawbone model, which was then 3D printed to create a bone cutting guide plate. Twenty-one New Zealand white rabbits were used to construct a rabbit mandibular bone defect model. Two bone defect areas were prepared on both sides of each rabbit, with one side of the bone defect area consisting of autogenous bone and artificial bone powder and a 1:1 mixture of autogenous bone powder and artificial bone powder was implanted. The other bone defect area was taken as the autologous bone powder group, with only autologous bone powder material implanted. Angiogenesis and osteogenesis in both bone defect areas at 2, 5, 7, 14 days and 4, 8, 12 weeks after modeling were detected.
RESULTS AND CONCLUSION: (1) At 12 weeks after surgery, the histological morphology and angiogenesis of the autologous bone powder group were similar to those of the autologous bone powder + artificial bone powder group. (2) The number of vascular cross-sections in the autologous bone powder group showed a more significant upward trend from 2 to 7 days, while the change trends of the number of vascular cross-sections in the two groups were similar in other periods. (3) At 8 weeks after surgery, the distribution range of new bone in the autologous bone powder + artificial bone powder group was smaller than that in the autologous bone powder group. At 12 weeks, the continuity and compactness of the bone tissue in both groups were good, close to the morphology of normal bone tissue. (4) At 8 weeks, the bone volume fraction of the autologous bone powder group was significantly higher than that of the autologous bone powder + artificial bone powder group (P < 0.05). By 12 weeks, there was no significant difference in the bone volume parameters between the autologous bone powder + artificial bone powder group and the autologous bone powder group (P > 0.05). These results indicate that angiogenesis and bone regeneration are more obvious in the early stage after implantation of autologous bone powder. However, by 12 weeks after surgery, the mixture of autologous bone powder and artificial bone powder is similar to pure autologous bone powder implantation in the repair of rabbit mandibular defects. This suggests that as the bone remodeling process proceeds, the comprehensive effects of the two materials tend to be consistent.


Key words: guided bone regeneration, angiogenesis, osteogenesis, bone defect repair, autologous bone powder, animal experiment

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