Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (11): 1664-1669.doi: 10.3969/j.issn.2095-4344.3108
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Zhao Ning1, Yu Hongdan2, Feng Zhen2, Ding Jiayuan2, Liu Xuezheng1
Received:
2020-04-03
Revised:
2020-04-28
Accepted:
2020-05-27
Online:
2021-04-18
Published:
2020-12-21
Contact:
Liu Xuezheng, MD, Professor, College of Integrative Chinese and Western Medicine, Liaoning University of Traditional Chinese Medicine, Shenyang 110847, Liaoning Province, China
About author:
Zhao Ning, Master, Lecturer, College of Integrative Chinese and Western Medicine, Liaoning University of Traditional Chinese Medicine, Shenyang 110847, Liaoning Province, China
CLC Number:
Zhao Ning, Yu Hongdan, Feng Zhen, Ding Jiayuan, Liu Xuezheng. Salidroside inhibits apoptosis of retinal Müller cells induced by high glucose in rats[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(11): 1664-1669.
2.5 红景天苷对高糖作用后rMC-1细胞Cleaved caspase-3、PI3K、Akt 、P-Akt蛋白表达的影响 Western blot检测各组细胞Cleaved caspase-3、PI3K、Akt 、P-Akt蛋白的表达情况,见图5。与正常对照组相比,高糖损伤组Cleaved caspase-3蛋白表达明显升高(P < 0.05), PI3K 蛋白表达明显减少(P < 0.01)。以总Akt为参照,P-Akt表达明显减少(P < 0.01);与高糖损伤组相比,红景天苷组Cleaved caspase-3蛋白表达明显减少,PI3K 蛋白表达显著升高,以总Akt为参照,P-Akt表达明显增多(P < 0.05);而PI3K 抑制剂组结果与红景天苷组结果相反(P < 0.05)。以上结果提示红景天苷可能通过激活AKT信号通路减少高糖作用后rMC-1细胞Cleaved caspase-3 的表达,而LY294002 能阻止这种效果。 "
[1] SALEH I, MARITSKA Z, PARISA N, et al. Inhibition of Receptor for Advanced Glycation End Products as New Promising Strategy Treatment in Diabetic Retinopathy.Open Access Maced J Med Sci.2019;7(23): 3921-3924. [2] OSTRI C, LA COUR M, LUND-ANDERSEN H. Diabetic vitrectomy in a large type 1 diabetes patient population: long-term incidence and risk factors.Acta Ophthalmol. 2014;92(5):439-443. [3] CHEN H, JI Y, YAN X, et al. Berberine attenuates apoptosis in rat retinal Muller cells stimulated with high glucose via enhancing autophagy and the AMPK/mTOR signaling. Biomed Pharmacother. 2018;108: 1201-1207. [4] WANG JJ, ZHU M, LE YZ. Functions of Muller cell-derived vascular endothelial growth factor in diabetic retinopathy. World J Diabetes. 2015;6(5):726-733. [5] ZHOU J, SHEN X, LU Q, et al. Thioredoxin-Interacting Protein (TXNIP) Suppresses Expression of Glutamine Synthetase by Inducing Oxidative Stress in Retinal Muller Glia Under Diabetic Conditions.Med Sci Monit. 2016;22:1460-1466. [6] TIEN T, ZHANG J, MUTO T, et al. High Glucose Induces Mitochondrial Dysfunction in Retinal Muller Cells: Implications for Diabetic Retinopathy. Invest Ophthalmol Vis Sci. 2017;58(7):2915-2921. [7] OLA MS, AL-DOSARI D, ALHOMIDA AS. Role of Oxidative Stress in Diabetic Retinopathy and the Beneficial Effects of Flavonoids. Curr Pharm Des. 2018;24(19):2180-2187. [8] CHEN S, CAI F, WANG J, et al. Salidroside protects SHSY5Y from pathogenic alphasynuclein by promoting cell autophagy via mediation of mTOR/p70S6K signaling.Mol Med Rep. 2019;20(1):529-538. [9] ZHU L, CHEN T, CHANG X, et al. Salidroside ameliorates arthritis-induced brain cognition deficits by regulating Rho/ROCK/NF-kappaB pathway.Neuropharmacology. 2016;103:134-142. [10] TANG H, GAO L, MAO J, et al. Salidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-kappaB and TGF-beta1/Smad-2/-3 pathways.Cell Stress Chaperones. 2016;21(2):239-249. [11] XING SS, YANG XY, ZHENG T, et al. Salidroside improves endothelial function and alleviates atherosclerosis by activating a mitochondria-related AMPK/PI3K/Akt/eNOS pathway.Vascul Pharmacol. 2015;72: 141-152. [12] SHI K, WANG X, ZHU J, et al. Salidroside protects retinal endothelial cells against hydrogen peroxide-induced injury via modulating oxidative status and apoptosis.Biosci Biotechnol Biochem. 2015;79(9):1406-1413. [13] QIAN C, LIANG S, WAN G, et al. Salidroside alleviates high-glucose-induced injury in retinal pigment epithelial cell line ARPE-19 by down-regulation of miR-138.RNA Biol. 2019;16(10):1461-1470. [14] BERGERHOFF K, CLAR C, RICHTER B. Aspirin in diabetic retinopathy.A systematic review. Endocrinol Metab Clin North Am. 2002;31(3): 779-793. [15] EGAN A, BYRNE M. Effects of medical therapies on retinopathy progression in type 2 diabetes. Ir Med J. 2011;104(2):37. [16] WANG L, WANG N, TAN HY, et al. Protective effect of a Chinese Medicine formula He-Ying-Qing-Re Formula on diabetic retinopathy.J Ethnopharmacol. 2015;169:295-304. [17] ZHAO YQ, LI QS, XIANG MH, et al. Distribution of traditional Chinese medicine syndromes of diabetic retinopathy and correlation between symptoms . Zhongguo Zhong Yao Za Zhi. 2017;42(14):2796-2801. [18] LU H,LI Y,ZHANG T,et al.Salidroside Reduces High-Glucose-Induced Podocyte Apoptosis and Oxidative Stress via Upregulating Heme Oxygenase-1 (HO-1) Expression.Med Sci Monit.2017;23:4067-4076. [19] MA YG, WANG JW, ZHANG YB, et al. Salidroside improved cerebrovascular vasodilation in streptozotocin-induced diabetic rats through restoring the function of BKCa channel in smooth muscle cells.Cell Tissue Res. 2017;370(3):365-377. [20] 韩雪娇.郭娜.朱美宣.等.红景天苷药理作用及其作用机理研究进展[J].中国生化药物杂志,2015,35(1):171-175. [21] NEWMAN E, REICHENBACH A. The Muller cell:a functional element of the retina.Trends Neurosci. 1996;19(8):307-312. [22] OLA MS. Effect of hyperglycemia on insulin receptor signaling in the cultured retinal Muller glial cells.Biochem Biophys Res Commun. 2014; 444(2):264-269. [23] BRINGMANN A, PANNICKE T, GROSCHE J, et al. Muller cells in the healthy and diseased retina. Prog Retin Eye Res. 2006;25(4):397-424. [24] FU QL, WU W, WANG H, et al. Up-regulated endogenous erythropoietin/erythropoietin receptor system and exogenous erythropoietin rescue retinal ganglion cells after chronic ocular hypertension. Cell Mol Neurobiol. 2008;28(2):317-329. [25] 颜赛梅,常青.糖尿病视网膜病变中Müller 细胞氧化还原状态研究[J].中国眼耳鼻喉科杂志,2009,9(4):261-263. [26] TRUDEAU K, MOLINA AJ, GUO W, et al. High glucose disrupts mitochondrial morphology in retinal endothelial cells: implications for diabetic retinopathy.Am J Pathol. 2010;177(1):447-455. [27] KOWLURU RA, KOWLURU A, MISHRA M, et al. Oxidative stress and epigenetic modifications in the pathogenesis of diabetic retinopathy.Prog Retin Eye Res. 2015;48:40-61. [28] LIU L, ZUO Z, LU S, et al. Naringin attenuates diabetic retinopathy by inhibiting inflammation, oxidative stress and NF-kappaB activation in vivo and in vitro. Iran J Basic Med Sci. 2017;20(7):813-821. [29] BRINGMANN A, PANNICKE T, GROSCHE J, et al. Muller cells in the healthy and diseased retina. Prog Retin Eye Res. 2006;25(4):397-424. [30] HAN N, YU L, SONG Z, et al. Agmatine protects Muller cells from high-concentration glucose-induced cell damage via N-methyl-D-aspartic acid receptor inhibition. Mol Med Rep. 2015;12(1):1098-1106. [31] REN X, SUN L, WEI L, et al. Liraglutide Up-regulation Thioredoxin Attenuated Muller Cells Apoptosis in High Glucose by Regulating Oxidative Stress and Endoplasmic Reticulum Stress.Curr Eye Res.2020; 17:1-9. [32] ZHOU X, AI S, CHEN Z, et al. Probucol promotes high glucose-induced proliferation and inhibits apoptosis by reducing reactive oxygen species generation in Muller cells.Int Ophthalmol. 2019;39(12):2833-2842. [33] DEVI TS, LEE I, HUTTEMANN M, et al. TXNIP links innate host defense mechanisms to oxidative stress and inflammation in retinal Muller glia under chronic hyperglycemia: implications for diabetic retinopathy.Exp Diabetes Res. 2012;2012:438238. [34] 庞若宇,关美萍,郑宗基,等.二甲双胍对糖基化终末产物诱导的成纤维细胞凋亡及相关蛋白Caspase3、Bax及Bcl-2 表达的影响[J].南方医科大学学报,2015,35(6):898-902. [35] ZUO T, ZHU M, XU W, et al. Iridoids with Genipin Stem Nucleus Inhibit Lipopolysaccharide-Induced Inflammation and Oxidative Stress by Blocking the NF-kappaB Pathway in Polycystic Ovary Syndrome.Cell Physiol Biochem. 2017;43(5):1855-1865. [36] CUI W, LENG B, WANG G. Klotho protein inhibits H2O2-induced oxidative injury in endothelial cells via regulation of PI3K/AKT/Nrf2/HO-1 pathways. Can J Physiol Pharmacol. 2019;97(5):370-376. [37] 师岩,徐晶,程昊,等.京尼平抑制高糖诱导的大鼠心肌H9c2细胞氧化应激及凋亡损伤[J].中国病理生理杂志,2019,35(2):224-230. [38] 崔伟,王高频,王洪新,等.黄芪甲苷通过激活PI3K/Akt通路抑制内皮细胞内质网应激介导的细胞凋亡的研究[J].中药药理与临床, 2018,34(5):39-44. [39] YANG X, HUO F, LIU B, et al. Crocin Inhibits Oxidative Stress and Pro-inflammatory Response of Microglial Cells Associated with Diabetic Retinopathy Through the Activation of PI3K/Akt Signaling Pathway.J Mol Neurosci. 2017;61(4):581-589. [40] YIN Y, LIU D, TIAN D. Salidroside prevents hydroperoxide-induced oxidative stress and apoptosis in retinal pigment epithelium cells.Exp Ther Med. 2018;16(3):2363-2368. [41] 陶蕊,张怀国,付庆喜,等.红景天苷对肌萎缩侧索硬化症的保护机制[J].中药药理与临床,2019,10(3):2471-2473. |
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