Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (27): 4275-4279.doi: 10.3969/j.issn.2095-4344.1372

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Effect of lycopene on proliferation and function of osteoblasts under oxidative stress

Rong Hui1, Xue Wenli2, Long Yanming2, Xie Mengsheng2, Cao Yong2, Li Xiaojie2   

  1.  (1the First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, Guangxi Zhuang Autonomous Region, China; 2College of Stomatology, Guangxi Medical University, Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Clinical Research Center for Craniofacial Deformity, Guangxi Key Laboratory of Oral and Maxillofacial Surgery Disease Treatment, Nanning 530021, Guangxi Zhuang Autonomous Region, China)
  • Received:2019-04-25 Online:2019-09-28 Published:2019-09-28
  • Contact: Cao Yong, Master, Associate chief physician, College of Stomatology, Guangxi Medical University, Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Clinical Research Center for Craniofacial Deformity, Guangxi Key Laboratory of Oral and Maxillofacial Surgery Disease Treatment, Nanning 530021, Guangxi Zhuang Autonomous Region, China Corresponding author: Li Xiaojie, MD, Associate professor, College of Stomatology, Guangxi Medical University, Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Clinical Research Center for Craniofacial Deformity, Guangxi Key Laboratory of Oral and Maxillofacial Surgery Disease Treatment, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • About author:Rong Hui, Master, Attending physician, the First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, Guangxi Zhuang Autonomous Region, China
  • Supported by:

    the Natural Science Foundation of Guangxi Zhuang Autonomous Region, No. 2013GXNSFBA019162 (to LXJ); the Basic Ability Promotion Project of Young and Middle-Aged Teachers in Guangxi Universities, No. 2017KY0092 (to LXJ); the Youth Science Foundation of Guangxi Medical University, No. GXMUYF201634 (to CY)

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Abstract:

BACKGROUND: Oxidative stress is an important factor for bone metabolism, and oxidative stress in vivo caused by various diseases can lead to bone metabolism disorder. Regulating bone metabolism level in vivo by antioxidant is of great significance for preventing bone mass loss.
OBJECTIVE: To investigate the effect of lycopene on the proliferation, differentiation and mineralization of osteoblasts under oxidative stress.
METHODS: The study was approved by the Experimental Animal Ethics Committee of Guangxi Medical University, approval number: 201601007. The osteoblasts were obtained by enzymatic digestion in the calvarial cap of Sprague-Dawley neonatal mice, and were cultured and identified. The passage 3 cells were used for experiment. There were H2O2 group (24-hour culture in the medium containing 100 μmol/L H2O2, and then cultured in the 10% FBS), control group (cultured in the 10% FBS), 10, 102, 103 and 104 nmol/L lycopene groups (24-hour culture in the medium containing different concentrations of lycopene, 24-hour culture in the medium containing 100 μmol/L H2O2, and then cultured in the 10% FBS). The cells in each group were cultured for 21 days. The effects of different concentrations of lycopene on the proliferation, differentiation and mineralization of osteoblasts under the simulated oxidative stress of hydrogen peroxide were detected.
RESULTS AND CONCLUSION: (1) The cell proliferation in the 10-103 nmol/L lycopene prophylactic application groups was significantly higher than that in the H2O2 group (P < 0.05). The cell proliferation in the 104 nmol/L lycopene group at 3, 5 and 7 days of culture was significantly lower than that in the other groups (P < 0.05). (2) The alkaline phosphatase activity of 10-103 nmol/L lycopene prophylactic application groups was significantly higher than that in the H2O2 group (P < 0.05). (3) The 10-103 nmol/L lycopene prophylactic application groups had significantly higher cell mineralization ability than the H2O2 group (P < 0.05), and the higher the concentration of lycopene, the stronger the protective effect of the mineralization ability to the cell (P < 0.05). (4) To conclude, lycopene prophylactic application can reduce the effect of oxidative stress on proliferation, differentiation and mineralization of osteoblasts.

Key words: osteoblasts, lycopene, proliferation, differentiation, oxidative stress, mineralization, alkaline phosphatase

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