Osteogenic effects of hydroxyapatite/beta-tricalcium phosphate/chitosan/methylcellulose bone for maxillary sinus elevation

doi:10.3969/j.issn.2095-4344.1272

Abstract

Abstract:

BACKGROUND: Three-dimensional scaffold material hydroxyapatite/β-tricalcium phosphate/chitosan/methylcellulose has been shown to exhibit good physical and chemical properties and biocompatibility.

OBJECTIVE: To evaluate the osteogenic effects of injectable hydroxyapatite/β-tricalcium phosphate/chitosan/methylcellulose scaffold material compounded with bone marrow mesenchymal stem cells used for maxillary sinus elevation in rabbits.

METHODS: Twenty-four New Zealand rabbits were provided by Laboratory Animal Center of Xinjiang Medical University, China and included in this study. Four maxillary sinus elevation areas were created in each rabbit. Injectable hydroxyapatite/β-tricalcium phosphate/chitosan/ methylcellulose scaffold compounded with bone marrow mesenchymal stem cell complex (experimental group), simple injectable hydroxyapatite/β-tricalcium phosphate/chitosan/methylcellulose scaffold (control group 1), or hydroxyapatite /β-tricalcium phosphate bone powder (control group 2) were implanted into three of the four maxillary sinus elevation areas. For blank control, no material was implanted in the last area. At 4, 8 and 12 weeks after surgery, maxillary sinus elevation area specimens were taken for cone-beam CT scanning, hematoxylin-eosin staining, and immunohistochemical staining. The experiments were approved by the Laboratory Animal Ethics Committee of the First Affiliated Hospital of Xinjiang Medical University (approval No. IACUC201706-02).

RESULTS AND CONCLUSION: (1) Cone beam CT scanning showed that there was no newly formed bone in the blank control group, and new bone formed in the other three groups, in particular in the experimental group, at 8 weeks after surgery. A greater amount of bone formed in the experimental group, control group 1, and control group 2 at 12 weeks after surgery compared with the blank control group. There was no significant difference among three groups. (2) Hematoxylin-eosin staining showed that at 8 and 12 weeks after surgery, the osteogenic effect in the experimental group was superior to that in the control groups 1 and 2 (P < 0.05). (3) Immunohistochemical staining showed that at 8 weeks after surgery, the expression level of osteogenic markers bone morphogenetic protein 2 and vascular endothelial growth factor in the experimental group was higher than that in the control groups 1 and 2 (P < 0.05). At 12 weeks after surgery, there were no significant differences in the expression levels of bone morphogenetic protein 2 and vascular endothelial growth factor among three groups (P > 0.05). (4) These results suggest that injectable hydroxyapatite/β-tricalcium phosphate/chitosan/methylcellulose scaffolds can promote the bone formation in the maxillary sinus elevation areas.

Key words: hydroxyapatite/β-tricalcium phosphate/chitosan/methylcellulose scaffold, hydroxyapatite/β-tricalcium phosphate, injectable material, bone morphogenetic protein 2, vascular endothelial growth factor, bone marrow mesenchymal stem cells, tissue engineering, maxillary sinus elevation, osteogenic effect

CLC Number:

Diao Zhaofeng, Li Xiaoliang, Ailimaierdan•Aniwear, Lin Cheng, Zhou Mei, Pan Xiaoling, Li Peng. Osteogenic effects of hydroxyapatite/beta-tricalcium phosphate/chitosan/methylcellulose bone for maxillary sinus elevation[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(22): 3456-3462.

Figures/Tables 8

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