Lingo-1 regulates neural stem cell differentiation via Rho-ROCK signaling pathway

doi:10.3969/j.issn.2095-4344.0625

Abstract

Abstract:

BACKGROUND: It has been reported that Lingo-1 plays an important role in neuronal axon regeneration and myelination, but whether it participates in the regulation of neural stem cell differentiation remains unknown.
OBJECTIVE: To examine the effect of Lingo-1 knockdown on the differentiation of neural stem cells and to explore its potential mechanism.
METHODS: Neural stem cells were isolated from neonatal rat brain tissue and identified by determination of several neural stem cell specific markers. Then the cells were transfected with Lingo-1 shRNA lentivirus or negative control vectors, and were further cultured in induced differentiation medium for 7 days. Immunofluorescence and western blot techniques were thereafter used to detect the expression of neuron specific markers and to examine expression levels of RhoA and ROCK1.
RESULTS AND CONCLUSION: The cells isolated from brain tissue of neonatal rats aggregated to form non-adherent spherical clusters with high expression of Nestin, Sox2, and Pax6, specific markers of neural stem cells, which exhibited the characteristics of neural stem cells. After transfected with lentivirus, expression of Lingo-1 was successfully knocked down at mRNA and protein levels. After 7 days of induction, expression of neuron specific markers was significantly increased in Lingo-1 knocked-down neural stem cells. Meanwhile, expressions of RhoA and ROCK1 were decreased. Thus, Lingo-1 can be involved in the differentiation of neural stem cells. Inhibition of Lingo-1 can induce neural stem cells to differentiate into neurons, which is possibly related to the regulation of Rho-ROCK signaling pathway.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Neural Stem Cells, Cell Differentiation, Neurons, Tissue Engineering

CLC Number:

R394.2

Peng Zhi-ming, Zhao Xiao-yang, Zhu Kai, Wan Yong. Lingo-1 regulates neural stem cell differentiation via Rho-ROCK signaling pathway[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(29): 4669-4674.

Figures/Tables 2

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