Expression of tumor stem cell surface marker CD44 in gastric cancer tissues and its significance

doi:10.3969/j.issn.2095-4344.2016.45.011

Abstract

Abstract:

BACKGROUND: Studies have shown that CD44 expression is closely associated with malignant transformation of gastric cancer.
OBJECTIVE: To study the expression of CD44 in gastric cancer tissues and its clinical significance.
METHODS: Gastric cancer specimens from 94 cases of gastric cancer after surgery and normal gastric tissue specimens from 30 cases undergoing gastroscope examination were collected and detected using S-P method. The positive expression rate of CD44 protein in these two groups was compared, and the relationship between CD44 and prognosis was analyzed.
RESULTS AND CONCLUSION: The positive expression rate of CD44 protein in the gastric cancer group (73%) was significantly higher than that in the normal group (3%) (P < 0.05). The positive expression rate of CD44 protein in gastric cancer patients was remarkably associated with TNM stage, differentiation degree, invasion depth and lymph node metastasis (P < 0.05). The 3-year survival rate of gastric cancer patients positive for CD44 protein was significantly lower than that of negative patients (P < 0.05). The higher TNM staging indicated the lower differentiation degree, and lymph node metastasis and CD44 positive expression were independent risk factors (P < 0.05). To conclude, the expression of CD44 protein is related to the clinical pathological features and prognosis of gastric cancer patients to some extents.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stomach Neoplasms, Neoplastic Stem Cells, Antigens, CD44, Tissue Engineering

CLC Number:

R394.2

Zhang Ze, Zeng Xiang-yong, Duan Shan. Expression of tumor stem cell surface marker CD44 in gastric cancer tissues and its significance[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(45): 6760-6765.

Figures/Tables 1

References

[1] 周雨,易德保,蒋传命,等.老年患者胃癌组织中CD44和ABCG2的表达及相关性[J].中国老年学杂志,2014, 34(24):7042-7044.
[2] 李威,姜波健,俞继卫.CD44与CD133蛋白在胃癌组织中的表达及其临床意义[J].中国普外基础与临床杂志,2014, 21(1):21-28.
[3] 黄玲,朱伟,龚希平,等.CD44在胃癌患者及胃癌细胞系中的表达及意义[J].临床检验杂志,2015,33(7):513- 515.
[4] 梁赅,杜伟,李爽,等.抑制HIF1α表达对低氧培养SGC- 7901胃癌细胞CD44表达的影响[J].实用癌症杂志,2015, 30(9):1269-1272.
[5] 刘建明,周友浪,马利林,等.Nanog与CD44在胃癌细胞株MKN45球体细胞中的表达及其意义[J].天津医药,2015, 43(1):30-33.
[6] 李士亭,陶文成.肿瘤干细胞表面标记物CD44在胃癌浸润和淋巴结转移中的作用[J]. 中国组织工程研究,2015, 19(23):3669-3673.
[7] 梁鹏,刘峰,孙业全,等.胃癌患者淋巴结转移对CD44v5表达的影响[J].医学影像学杂志,2014,24(2):330-331.
[8] 文峰,陈兰,程建国,等. Ezrin和CD44在胃癌组织中的表达及临床意义[J].实用临床医药杂志,2014,18(3):19-22.
[9] Duffy MJ, Lamerz R, Haglund C,et al. Tumor markers in colorectal cancer, gastric cancer and gastrointestinal stromal cancers: European group on tumor markers 2014 guidelines update.Int J Cancer. 2014;134(11): 2513-2522.
[10] 周雨,朱亚平,蒋传命.干细胞标志物CD44在胃癌中的表达及意义[J].中国肿瘤临床与康复,2015,22(6):672-674.
[11] Li X, Yao R, Yue L,et al. FOXM1 mediates resistance to docetaxel in gastric cancer via up-regulating Stathmin.J Cell Mol Med. 2014;18(5):811-823.
[12] 陈勇,张红,吴梦婕,等.CD44 v6和FOXD3在人胃癌组织中的表达及临床意义[J].安徽医科大学学报,2015,50(12): 1795-1799.
[13] Wang YW, Shi DB, Chen X,et al. Clinicopathological significance of microRNA-214 in gastric cancer and its effect on cell biological behaviour. PLoS One. 2014; 9(3):e91307.
[14] 魏世平,张俊会.姜黄素对裸鼠人胃癌细胞MMP-2和CD44v6在mRNA水平表达的影响[J].河北医药,2015, 37(16):2412-2415.
[15] García-González MA, Lanas A.Genetic susceptibility and gastric cancer risk: the importance of meta-analyses as a statistical tool.Gastroenterol Hepatol. 2014;37(7):421-426.
[16] Pirogov SS, Sokolov VV, Karpova ES, et al. Early gastric cancer and precancerous conditions diagnostics with confocal laser endomicroscopy.Eksp Klin Gastroenterol. 2014;(3):18-24.
[17] 李延海,孙辉,任鹏.新辅助化疗对胃癌组织CD44V6、p21阳性表达及患者预后的影响[J].山东医药,2016,56(8): 38-39.
[18] 宋述安,王宁,赵志威,等. Survivin、Dnmt和CD44在胃溃疡及胃癌中表达的差异[J]. 现代生物医学进展,2015, 15(10):1954-1956.
[19] 杨宏琼,陆亚华,周志华,等.人胃癌细胞株BGC-823克隆形态观察及其CD44表达变化[J].山东医药,2015,55(34): 24-25.
[20] 宣立功,武英.VEGF、CD44V6和ICAM-1在胃癌组织中的表达及意义[J].中医学报, 2014,29(7):533-534.
[21] 刘元华,樊祥山,恽艳.应用组织芯片技术检测胃癌组织中Lgr5和CD44的表达[J]. 中国肿瘤外科杂志,2015,7(6): 341-345.
[22] Ji YN, Wang Q, Li Y, et al. Prognostic value of vascular endothelial growth factor A expression in gastric cancer: a meta-analysis.Tumour Biol. 2014;35(3): 2787-2793.
[23] 王永辉,毛卫波,楼建,等.CD44V6、HER2及Ki67在胃癌和癌旁组织中的表达及相关性分析[J].浙江实用医学,2014, 19(3):160-164.
[24] 杨家佳,张琳,郑茂金,等.肿瘤干细胞标记物CD44和EpCAM在胃癌中的表达及意义[J].徐州医学院学报, 2014,34(10):661-664.
[25] 杨熙华.细胞标志物CD133、CD44在胃癌中的表达与预后的关系[D].南宁:广西医科大学,2014.
[26] Okazaki M, Fushida S, Harada S,et al. The angiotensin II type 1 receptor blocker candesartan suppresses proliferation and fibrosis in gastric cancer. Cancer Lett. 2014;355(1):46-53.
[27] 恽艳.肿瘤干细胞标志Lgr5和CD44在胃癌中的表达及临床病理意义[D].苏州:苏州大学,2014.
[28] 王文杰. NHERF1在胃癌组织的表达及其与浸润、转移关系的研究[D].河北:河北医科大学,2014.
[29] Galletti G, Sung MS, Vahdat LT,et al. Isolation of breast cancer and gastric cancer circulating tumor cells by use of an anti HER2-based microfluidic device.Lab Chip. 2014;14(1):147-156.
[30] Chen Z, Zhang L, Xia L,et al. Genomic analysis of drug resistant gastric cancer cell lines by combining mRNA and microRNA expression profiling.Cancer Lett. 2014; 350(1-2):43-51.
[31] 李禄椿. Notch1信号转导通路对CD44+胃癌细胞生物学特性的影响[D].重庆:重庆医科大学,2014.
[32] Lin L, Jiang H, Huang M,et al. Depletion of histone deacetylase 1 inhibits metastatic abilities of gastric cancer cells by regulating the miR-34a/CD44 pathway. Oncol Rep. 2015;34(2):663-672.
[33] 李志海.胃癌细胞多药耐药相关基因的表达与其侵袭转移能力的相关性研究[J].现代消化及介入诊疗,2014, 19(4):218-221.
[34] 陈兵. CD44v6在胃癌、癌前病变及癌旁组织中的表达及其临床意义[D].合肥:安徽医科大学,2014.
[35] 王宏.腹腔镜胃癌手术腹腔冲洗液中sICAM-1、sCD44v6表达的影响及其意义[J].国际肿瘤学杂志,2014, 41(11): 872-874.