Electroacupuncture promotes the proliferation and differentiation of neural stem cells in the rat hippocampus of spleen deficiency syndrome

doi:10.3969/j.issn.2095-4344.2016.32.022

Abstract

Abstract:

BACKGROUND: Spleen deficiency has varying impact on the immune system and digestive system of the human body, and can also damage the normal function of the central nervous system as there is a close relationship between the spleen and brain.
OBJECTIVE: To explore the effect of electroacupuncture on the proliferation and differentiation of neural stem cells in the rat hippocampus of spleen deficiency syndrome.
METHODS: Sixty Sprague-Dawley rats were randomly divided into three groups: normal, control and electroacupuncture groups. The animal model of spleen deficiency syndrome was prepared in the control group and electroacupuncture group. Two weeks after modeling, rats in the electroacupuncture group were given electroacupuncture treatment, and the changes of body mass in normal rats and model rats were measured. Five rats from each group were taken to observe the histological changes of neural stem cells in the hippocampus at 1, 2, 3, 4 weeks after treatment.
RESULTS AND CONCLUSION: The body mass of rats in the model group was significantly lower than that in the normal group at 2 weeks after modeling (P < 0.05). In the electroacupuncture group, the number of BrdU positive cells was significantly higher than that in the normal group and control group at 2, 3, 4 weeks after modeling (P < 0.05); the number of BrdU/Nestin positive cells was significantly higher than that in the normal group at 2, 3, 4 weeks (P < 0.05); the number of BrdU/glial fibrillary acidic protein positive cells was significantly higher than that in the normal group at 2 weeks (P < 0.05); and the number of BrdU/neuronspecific enolase positive cells was significantly higher than the normal group at 3 weeks (P < 0.05). In the control group, the number of BrdU/Nestin positive cells was significantly lower than that in the normal group and electroacupuncture group at 1 week after modeling, significantly lower than that in the electroacupuncture group at 2 and 4 weeks, and significantly lower than the normal group at 3 weeks (P < 0.05); the number of BrdU/glial fibrillary acidic protein positive cells was significantly lower than that in the normal group and electroacupuncture group at 1, 2, 4 weeks; and the number of BrdU/neuronspecific enolase positive cells was significantly lower than that in the normal group and electroacupuncture group at 1, 2 weeks. Taken together, these experimental findings show that electroacupuncture treatment effectively promotes the proliferation of hippocampal neural stem cells that are induced to differentiate into astrocytes and neurons.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stem Cells, Neural Stem Cells, Hippocampus, Cell Differentiation

CLC Number:

R394.2

Miao Ling. Electroacupuncture promotes the proliferation and differentiation of neural stem cells in the rat hippocampus of spleen deficiency syndrome[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(32): 4859-4864.

Figures/Tables 1

2.1 实验流程与动物数量分析入组的动物均进入最后的结果分析，未出现死亡和脱落现象。 2.2 脾虚证大鼠造模情况大鼠在造模后于第2天，均开始出现不同程度的便澹，之后逐渐食量下降、精神倦怠、反应迟钝，排便次数不断增加，不断消瘦。正常组无异常表现，正常饮食、饮水。造模前，正常大鼠与模型组大鼠体质量无显著性意义(P > 0.05)。造模后2周，模型组显著低于正常组(P < 0.05)。见表1。 2.3 三组大鼠不同时间海马区BrdU阳性细胞数正常组大鼠BrdU阳性细胞数基本保持不变，不同时间比较差异均无显著性意义(P > 0.05)。对照组1，2周BrdU阳性细胞数显著低于同期正常组和电针组(P < 0.05)，3，4周显著低于同期电针组(P < 0.05)，但与正常组之间差异无显著性意义(P > 0.05)。2，3，4周，电针组BrdU阳性细胞数均显著高于正常组(P < 0.05)，见表2。 2.4 三组大鼠不同时间海马区BrdU/巢蛋白阳性细胞数正常组大鼠BrdU/巢蛋白阳性细胞数基本保持不变，不同时间比较差异均无显著性意义(P > 0.05)。对照组1周BrdU/巢蛋白阳性细胞数显著低于同期正常组和电针组，2，4周显著低于同期电针组，3周显著低于同期正常组(P < 0.05)。2，3，4周，电针组BrdU/巢蛋白阳性细胞数均显著高于正常组(P < 0.05)，见表3。 2.5 三组大鼠不同时间海马区BrdU/胶质纤维酸性蛋白阳性细胞数正常组大鼠BrdU/胶质纤维酸性蛋白阳性细胞数基本保持不变，不同时间比较差异无显著性意义(P > 0.05)。对照组1，2，4周BrdU/胶质纤维酸性蛋白阳性细胞数显著低于同期正常组和电针组，2周电针组BrdU/胶质纤维酸性蛋白阳性细胞数显著高于正常组(P < 0.05)，见表4。 2.6 三组大鼠不同时间海马区BrdU/神经元特异性烯醇化酶阳性细胞数正常组大鼠Brdu/神经元特异性烯醇化酶阳性细胞数基本保持不变，不同时间比较差异无显著性意义(P > 0.05)。对照组1周、2周BrdU/神经元特异性烯醇化酶阳性细胞数显著低于同期正常组和电针组，3周电针组BrdU/神经元特异性烯醇化酶阳性细胞数显著高于正常组(P < 0.05)，见表5。"

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