Bone marrow mesenchymal stem cell transplantation for rat cerebral infarction: recovery of neurological function and expression of synaptophysin

doi:10.3969/j.issn.2095-4344.2016.28.012

Abstract

Abstract:

BACKGROUND: Synaptophysin plays an important role in the recovery of neural function after cerebral ischemia.
OBJECTIVE: To investigate the effects of bone marrow mesenchymal stem cell transplantation on nervous function and expression of synaptophysin after cerebral infarction.
METHODS: Totally 60 rats were equivalently randomized into four groups, including sham operation, control, model and stem cell treatment groups. Rats in the control, model and stem cell treatment groups were used for preparing cerebral infarction models, and the remaining underwent the sham operation. After 1 day of modeling, bone marrow mesenchymal stem cells were transplanted into the rat lateral ventricle in the stem cell treatment group, and rats in the control group was given the injection of the same amount of PBS. After 1, 7 and 14 days of treatment, rat’s neurological function was scored on beam-walking test, rotarod test and screen test, and expression of synaptophysin was detected by RT-PCR and immunohistochemical assay.
RESULTS AND CONCLUSION: At 7 and 14 days after treatment, the beam-walking test, rotarod test and screen test scores in the stem cell treatment group were significantly lower than those in the control and model groups (P < 0.05), and the above scores showed no significant differences between the control group and model group (P > 0.05). At 1 day after treatment, the mRNA expression of synaptophysin and the number of synaptophysin-positive cells in the sham operation group were significantly higher than those in the other three groups (P < 0.05); at 7 and 14 days after treatment, the mRNA expression of synaptophysin and the number of synaptophysin-positive cells in the stem cell treatment group were significantly increased compared with the other three groups (P < 0.05), and additionally, the mRNA expression of synaptophysin and the number of synaptophysin-positive cells in the sham operation group were significantly lower than those in the model and control groups (P < 0.05). These findings suggest that bone marrow mesenchymal stem cell transplantation can effectively promote the recovery of neurological function in cerebral infarction rats, and partially promote the formation of synaptophysin.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stem Cells, Stem Cell Transplantation, Cerebral Infarction

CLC Number:

R394.2

Cheng Lv-fang. Bone marrow mesenchymal stem cell transplantation for rat cerebral infarction: recovery of neurological function and expression of synaptophysin[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(28): 4182-4188.

Figures/Tables 2

2.1 实验动物数量分析入组动物全部进行进入最终的结果分析，未出现死亡动物。 2.2 骨髓间充质干细胞培养结果对大鼠骨髓间充质干细胞进行培养，连续培养7 d，细胞呈现出长梭形(见图1)，经传代培养，细胞迅速生长，并大多呈梭形。流式细胞术检测所培养的细胞高表达骨髓间充质干细胞表面标志分子CD44和CD90分别为97.1%和96.3%，而极低表达造血细胞表面分子CD45(2.5%)和CD34 (2.1%)。 2.3 神经功能评估结果治疗后1 d，对照组、模型组、干细胞治疗组之间平衡木行走实验、转棒上行走实验、网屏实验评分经比较差异均无显著性意义(P > 0.05)；治疗后7 d和14 d，干细胞治疗组平衡木行走实验、转棒上行走实验、网屏实验评分均显著低于对照组和模型组(P < 0.05)，对照组、模型组之间比较差异无显著性意义(P > 0.05)，具体结果见表1。 2.4 RT-PCR检测各组突触素表达假手术组各时间点突触素mRNA表达基本保持不变，模型组、对照组、干细胞治疗组均随时间推移呈现出不断上升的情况，且模型组和对照组变化情况基本一致(见图2)。经统计学比较可得治疗后1 d，假手术组的突触素mRNA表达均显著高于其他3组(均P < 0.05)；治疗后7 d和14 d，干细胞治疗组的突触素mRNA表达均显著高于其他3组(均P < 0.05)，且假手术组的突触素mRNA表达均显著低于模型组和对照组(均P < 0.05)，模型组和对照组各时间点比较差异均无显著性意义(P > 0.05)。具体结果见表2。 2.5 免疫组织化学检测各组突触素表达结果免疫组织化学检测突触素表达阳性细胞数，假手术组各时间点阳性细胞数基本保持不变，模型组、对照组、干细胞治疗组均随时间推移呈现出不断上升的情况，且模型组和对照组变化情况基本一致(见图3)。经统计学比较可得治疗后1 d，假手术组的阳性细胞数均显著大于其他3组(均P < 0.05)；治疗后7 d和14 d，干细胞治疗组的阳性细胞数均显著大于其他3组(均P < 0.05)，且假手术组的阳性细胞数均显著小于模型组和对照组(均P < 0.05)，模型组和对照组各时间点比较差异均无显著性意义(P > 0.05)，与突触素表达情况RT-PCR检测结果一致，具体结果见表3。治疗后14 d，经观察，在假手术组大脑皮质中可观察到突触素阳性细胞，但分散存在，数量较少，对照组在额顶皮质下脑组织及梗死灶周围纹状体均可观察到少量的突触素阳性细胞，干细胞治疗组则可观察到大量的突触素阳性细胞，具体结果见图4。"

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