Protective effect of bone marrow stromal stem cells against acute lung injury after hip fracture in aged rats with chronic obstructive pulmonary disease

doi:10.3969/j.issn.2095-4344.2016.28.013

Abstract

Abstract:

BACKGROUND: The elderly hip fracture patients with chronic obstructive pulmonary disease (COPD) have a higher postoperative mortality than those only with hip fractures. In recent years, it has become an issue of concerns. Because the mechanism is unknown, however, there are no effective clinical interventions for these patients.
OBJECTIVE: To observe the effect of bone marrow stromal stem cells (BMSCs) on the level of pulmonary inflammation in aged rats with chronic obstructive pulmonary disease (COPD) after hip fractures.
METHODS: Thirty male Sprague-Dawley rats, 12 months old, were exposed to smoking for 4 months and randomized into three groups, smoking, smoking+hip fracture+normal saline, smoking+hip fracture+ BMSCs groups. Animal models of hip fracture were made in the latter two groups. Twenty-four hours after hip fracture, the lower lobe and peripheral blood samples were taken from all rats in the three groups, to evaluate the pathological changes of lung tissue and detect levels of inflammatory factors in the lung tissue and peripheral blood.
RESULTS AND CONCLUSION: After closed hip fracture, aged COPD rats exhibited inflammatory cell infiltration, mucus secretion, airway stenosis or occlusion; the levels of interleukin-6, tumor necrosis factor-α and interleukin-10 in the lung tissue and peripheral blood were increased. After intravenous injection of BMSCs, the pathological changes of the lung tissue were reduced, and the levels of pro-inflammatory factors, tumor necrosis factor-α and interleukin-6, decreased, but the level of anti-inflammatory factor interleukin-10 further increased, which were significantly different from those in the normal saline group (P < 0.05). These findings indicate that BMSCs can relieve acute lung injury in aged COPD rats with hip fractures．

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Pulmonary Disease, Chronic Obstructive, Hip Fractures, Acute Lung Injury, Bone Marrow, Mesenchymal Stem Cell Transplantation, Tissue Engineering

CLC Number:

R394.2

Zhang Gui-tong, Feng Jie-yang, Liu Jia, Zhang Yan-jin, Xiang Zi-min, Meng Fan-xing,Sun Tian-sheng. Protective effect of bone marrow stromal stem cells against acute lung injury after hip fracture in aged rats with chronic obstructive pulmonary disease[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(28): 4189-4195.

Figures/Tables 1

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