Differentiation and culture of tumor stem cells in human primary gastric cancer cells

doi:10.3969/j.issn.2095-4344.2016.23.005

Abstract

Abstract:

BACKGROUND: There is a close relationship between tumor stem cells and tumor occurrence and recurrence, but there are still some disputes in the presence of tumor stem cells in all tumors.
OBJECTIVE: To investigate the differentiation and culture of tumor stem cells in human primary gastric cancer cells.
METHODS: Primary gastric cancer cells isolated from fresh gastric cancer tissues were stained with hematoxylin-eosin and identified by immunohistochemical detection of carcinoembryonic antigen. The CD44 expression of the cells was detected using immunofluorescence method. Magnetic activated cell sorting was used to isolate CD44⁺ gastric cancer cells that were then seeded subcutaneously behind the armpit of mice. Growth of implanted tumor cells was observed.
RESULTS AND CONCLUSION: Human primary gastric cancer cells were isolated in serum-free medium. Compared with the routine culture group, the number of CD44⁺ cells (P < 0.05) and the tumor volume were significantly increased in the spheroid culture group. Furthermore, at 90 days after transplantation, the tumor volume of mice in spheroid culture group was significantly higher than that in the routine culture group. These experimental findings indicate that gastric cancer cells with certain tumorigenicity can be successfully isolated from gastric cancer cells using serum-free culture method and magnetic activated cell sorting method.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stomach Neoplasms, Neoplastic Stem Cells, Cell Differentiation, Tissue Engineering

CLC Number:

R394.2

Wang Ying. Differentiation and culture of tumor stem cells in human primary gastric cancer cells[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(23): 3380-3385.

Figures/Tables 1

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