Effects of leaves of Lycium barbarum on estrogen receptor in ovariectomized rats

doi:10.3969/j.issn.2095-4344.2016.15.007

Abstract

Abstract:

BACKGROUND: Lycium barbarum polysaccharide, the main active component of leaves of lycium barbarum, has a remarkable therapeutic effect on osteoporosis in adult ovariectomized female rats.
OBJECTIVE: To explore effects of leaves of Lycium barbarum on serum estradiol and bone estrogen receptor expressions in adult oxariectomized female rats．
METHODS: Thirty-two 6-month-od female rats were randomized into sham-surgery, model, and treatment groups, respectively. Rat models of postmenopausal osteoporosis were induced by ovariotomy followed by orally administration of water solution of leaves of Lycium barbarum (500 or 1 000 mg/kg) or distilled water once daily for consecutive 12 weeks in treatment and model groups, respectively. Serum estradiol level was detected by radiation immunoassay method. Estrogen receptor α, β immunoreactivities in bone tissue were determined by immunohistochemical staining．
RESULTS AND CONCLUSION: Serum estradiol level in the model group was significantly decreased compared with the sham-surgery group (P < 0.01), but that was significantly increased in the treatment groups, particularly at high dose, compared with the model group (P < 0.01). Estrogen receptor α, β immunoreactivities were weaker in the model group than the sham-surgery group (P < 0.01), and those were stronger in the treatment groups than the model group (P < 0.01). Our results suggest that leaves of Lycium barbarum treat osteoporosis through enhancing serum estradiol and estrogen receptor expressions in adult ovariectomized rats．
中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Lycium, Osteoporosis, Osteoblasts, Receptors, Estrogen, Tissue Engineering

Ma Feng, Ma Jing-zu, Gong Fan, Gao Jun, Lv Jin-han. Effects of leaves of Lycium barbarum on estrogen receptor in ovariectomized rats[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(15): 2178-2183.

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