Human umbilical cord mesenchymal stem cells induced by 5-azacytidine: bioactive properties and cardiomyocyte-like differentiation

doi:10.3969/j.issn.2095-4344.2016.14.021

Abstract

Abstract:

BACKGROUND: Human umbilical cord mesenchymal stem cells belong to a special class of stem cells with a limited number, which are difficult to isolate and purify. Importantly, there is a lack of clinical understanding of biological characteristics of human umbilical cord mesenchymal stem cells.

OBJECTIVE: To study the bioactive properties and cardiomyocyte-like differentiation of human umbilical cord mesenchymal stem cells.

METHODS: Under a sterile collection, umbilical cord blood of newborn from cesarean section was collected to isolate human mesenchymal stem cells by digestion using collagenase II. Immunohistochemical staining was used to detect cell surface markers and observe morphological characteristics, and MTT assay used to detect cell proliferation and draw cell growth curve. Cells grown to 70%-80% confluence were treated with 10 μmol/L 5-azacytidine for 24 hours.

RESULTS AND CONCLUSION: One week after adherent tissue culture, human umbilical cord mesenchymal stem cells were seen, and then the cells cultured in culture medium showed colony growth. Within 24 hours of cell seeding, cells at passages 3 and 10 were at latency period and then grew logarithmically with a doubling time of 30 hours. Immunocytochemical staining showed that passage 3 cells at over 90% confluence expressed CD44 and CD29, rather than CD28 and CD31.Three weeks after induced culture, the cells with the absence of processes grew irregularly; after 4 weeks, the cells were round in shape and expressed cardiac-specific immune markers. Taken together, isolated human umbilical cord mesenchymal stem cells with strong proliferative ability have the basic biological characteristics of mesenchymal stem cells, which are capable of differentiating into cardiomyocyte-like cells induced by 5-azacytidine.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stem Cells, Adult Stem Cells, Tissue Engineering

Li Ping, Huangpu Chun-rong, Tang Chun-hui, Chen Yi-hui. Human umbilical cord mesenchymal stem cells induced by 5-azacytidine: bioactive properties and cardiomyocyte-like differentiation[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(14): 2117-2122.

Figures/Tables 5

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