Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (5): 783-787.doi: 10.3969/j.issn.2095-4344.2015.05.022

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Pyruvate effects on expression of intercellular adhesion molecular 1 in rats with grafted small bowel

Hao Zhi-qiang1, Zhang Tao1, Li Meng-bin2, Wang Wei-zhong2, Zhang Hong-wei2   

  1. 1Department of General Surgery, the 230th Hospital of Chinese PLA, Dandong 118000, Liaoning Province, China
    2Xijing Hospital of Digestive Diseases, the Fourth Military Medical University of Chinese PLA, Xi’an 710032, Shaanxi Province, China
  • Revised:2014-11-02 Online:2015-01-30 Published:2015-03-02
  • Contact: Zhang Tao, Department of General Surgery, the 230 th Hospital of Chinese PLA, Dandong 118000, Liaoning Province, China
  • About author:Hao Zhi-qiang, Master, Associate chief physician, Department of General Surgery, the 230th Hospital of Chinese PLA, Dandong 118000, Liaoning Province, China

Abstract:

BACKGROUND: Grafted small bowels are sensitive to ischemia-reperfusion injury, which is inevitable during transplantation process. Our previous study found the protective effect of pyruvate to rat grafted small bowel and investigated the underlying mechanisms.
OBJECTIVE: To study the relationship between pyruvate protective effect on ischemia-reperfusion injury and expression of intercellular adhesion molecular 1 of grafted small bowel.
METHODS: Adult male Sprague-Dawley rats of inbred line were randomly divided into three groups. Sham operation group: Rats underwent the laparotomy and the left nephrectomy. Ischemia/reperfusion group and pyruvate group: Ischemia/reperfusion rat models of small bowel transplantation were established and received lavage injection of nutrient solution only or nutrient solution with pyruvate, before donor intestinal blocking blood flow. Small intestinal tissue was harvested at 45, 90 minutes after ischemia and 30, 180 minutes after reperfusion. The pathological changes of small intestinal injury were observed under light microscope. The expression of intercellular adhesion molecular 1 was detected by immunohistochemistry in the small intestine tissue samples.
RESULTS AND CONCLUSION: The pathological injury of small intestine tissue at different time points of ischemia/reperfusion was severer in the ischemia/ reperfusion group than that in sham operation group and pyruvate group, and there was no significant difference in the latter two groups. The expression of intercellular adhesion molecular 1 stayed at a low level during ischemia period, and appeared sharp rise during reperfusion period, which was higher than that in the sham operation group and pyruvate group (P < 0.01). No significant differences were found between sham operation group and pyruvate group in the expression (P > 0.05). We conclude that pyruvate can afford protective effect to ischemia/reperfusion injury of grafted small bowel. The mechanism of protective effect of pyruvate is associated with decrease expression of intercellular adhesion molecular 1.



中国组织工程研究
杂志出版内容重点:肾移植肝移植移植;心脏移植;组织移植;皮肤移植;皮瓣移植;血管移植;器官移植组织工程


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Key words: Pyruvate, Reperfusion Injury, Intercellular Adhesion Molecular 1, Small Intestine, Transplantation, Models, Animal

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