Dermis-derived cell subpopulation is used to repair mouse calvarial defects

doi:10.3969/j.issn.2095-4344.2015.19.020

Abstract

Abstract:

BACKGROUND: In consideration of skin as the largest organ all over the body and its abundant vessels and vessel plexuses, there would be sufficient adult stem cells for tissue engineering.
OBJECTIVE: To investigate the osteogenic potential of dermis-derived bone morphogenetic protein receptor subtype IB (BMPR-IB) positive cells.
METHODS: In current study, histochemical analysis was adopted to study the localization and expression of BMPR-IB+ cells in skin. Fresh skin samples were digested into single cell suspension. Then, the surface marker BMPR-IB was used to isolate cell subpopulation by magnetic activated cell sorting from freshly prepared single cell suspension. After that, the osteogenic potential in vitro and in vivo was tested. Alkaline phosphatase staining and alizarin red staining were performed after osteogenic induction in vitro. The BMPR-IB+ cells were seeded onto coral scaffolds, and the scaffolds were used to repair critical-sized calvarial defects of mice. Histochemical
analysis was performed at 6 weeks postoperatively and micro-CT analysis was carried out at 24 weeks postoperatively to evaluate the ability of bone repairment.
RESULTS AND CONCLUSION: We localized BMPR-IB cells in situ by immunohistochemistry that turned out to be expressed in the reticular layer of dermis and by single cells. Cell subpopulation which expressed BMPR-IB could be sorted by magnetic activated cell sorting. Alkaline phosphatase staining was obviously positive and lots of calcium modules were confirmed by alizarin red staining after osteogenic induction, indicating that BMPR-IB+ cells had the osteogenic potential in vitro. Histochemical analysis demonstrated that plenty of new bone formation was found in BMPR-IB+ cells group after 6 weeks in vivo. Micro-CT analysis revealed that BMPR-IB+ cells-coral scaffold complex could repair calvarial defects successfully after 24 weeks in vivo. These results indicated that dermis-derived BMPR-IB+ cells possessed adequate osteogenic potential. Moreover, they might be promising seed cells for bone tissue engineering.

Key words: Skin;, Bone Morphogenetic Protein Receptors, Type I, Skull

CLC Number:

R394.2

Wang Ting-liang, He Jin-guang, Zhang Yang, Li Dan, Dong Jia-sheng, Zhu Lian. Dermis-derived cell subpopulation is used to repair mouse calvarial defects [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(19): 3067-3073.

Figures/Tables 6

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