Bone regeneration via a novel honeycomb-like polycaprolactone-calcium silicate crystal compound scaffold in extreme-sized cranial defects

doi:10.3969/j.issn.2095-4344.2017.18.011

Abstract

Abstract:

BACKGROUND: Polycaprolactone as a polymer material has poor biocompatibility, and needs to be combined with other natural biological materials to increase biocompatibility, thereby promoting tissue regeneration.

OBJECTIVE: To develop a novel honeycomb-like polycaprolactone-calcium silicate crystal compound scaffold, and observe its osteogenic effects in Sprague-Dawley rats with skull defects.

METHODS: Eighteen Sprague-Dawley rats were used to make skull defect models and randomized into three groups: blank control group with no implantation, control group with implantation of normal polycaprolactone-calcium silicate crystal compound scaffold, and experimental group with implantation of the novel honeycomb-like polycaprolactone- calcium silicate crystal compound scaffold. Six weeks after implantation, bone regeneration effect in the defect region measured via X-ray scanning, Micro-CT three-dimensional reconstruction, and histological analysis.

RESULTS AND CONCLUSION: (1) X-ray scan: in all the rats, the size of bone defect was reduced, the fracture line became vague, and the marginal bone density was increased. The percentage of new bone area was highest in the experimental group, successively followed by the control group and blank control group. (2) Micro-CT three-dimensional reconstruction: new bones in the blank control group were mainly distributed on the both sides of the defect, but those in the control and experimental groups distributed in the defect region. The bone regeneration capacity was ranked as follows: experimental group > control group > blank control group (P < 0.05). (3) Histological analysis: new bone ingrowth was visible in all the three groups to different extents. Compared with the other two groups, new bone formation and microvessel density were significantly higher in the experimental group (P < 0.05). To conclude, this novel honeycomb-like polycaprolactone-calcium silicate crystal compound scaffold can obviously promote bone formation in the skull defect region.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Skull, Bone Regeneration, Tissue Engineering

CLC Number:

R318

Song Bing, Liao Zhe-ting, Chen Yu-fan, Zhao Liang.

Bone regeneration via a novel honeycomb-like polycaprolactone-calcium silicate crystal compound scaffold in extreme-sized cranial defects

[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(18): 2852-2857.

Figures/Tables 6

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