Influence of skin-derived mesenchymal stem cells from psoriasis lesions on T cell proliferation

doi:10.3969/j.issn.2095-4344.2016.23.016

Abstract

Abstract:

BACKGROUND: Studies have found abnormalities in the biological activity and cytokine levels of bone marrow mesenchymal stem cells from psoriasis patients, while the biological activity of skin-derived mesenchymal stem cells from psoriasis lesions is rarely reported. OBJECTIVE: To explore the influences of skin-derived mesenchymal stem cells from psoriasis lesions on T cell proliferation
METHODS: After isolation and culture, skin-derived mesenchymal stem cells from psoriasis lesions and normal controls were separately co-cultured with peripheral blood T cells. T cell proliferation was detected using MTT assay, and levels of epidermal growth factor and transforming growth factor β1 in cell supernatant determined using ELISA method.
RESULTS AND CONCLUSION: Skin-derived mesenchymal stem cells from both psoriasis lesions and normal controls significantly inhibited T cell proliferation (P < 0.05). Furthermore, skin-derived mesenchymal stem cells from psoriasis lesions exhibited a weaker inhibitory effect on T cell proliferation than normal skin-derived mesenchymal stem cells (P < 0.05). Skin-derived mesenchymal stem cells from psoriasis lesions significantly increased epidermal growth factor but reduced transforming growth factor β1 compared with the normal skin-derived mesenchymal stem cells (P < 0.05). In conclusion, maybe the imbalances of growth factor levels due to skin injury elicit a decrease in the inhibitory effect of skin-derived mesenchymal stem cells from psoriasis lesions on T lymphocytes.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Psoriasis, Skin, Mesenchymal Stem Cells, T-Lymphocytes, Tissue Engineering

CLC Number:

R394.2

Wang Qing, Wang Da-hu, Wang Ai-xue, Guo Bing-shen, Wang Ning, Li Yu-ping. Influence of skin-derived mesenchymal stem cells from psoriasis lesions on T cell proliferation[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(23): 3451-3456.

Figures/Tables 2

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