Human umbilical cord mesenchymal stem cells: isolation, identification and transplantation combined with chemotherapy for lung cancer in mice

doi:10.3969/j.issn.2095-4344.2016.23.017

Abstract

Abstract:

BACKGROUND: Mesenchymal stem cells have pluripotent differentiation, and can promote cell engraftment and immune regulation. Therefore, we attempt to use human umbilical cord mesenchymal stem cells as a new source for treatment of lung cancer by exploring cell isolation, identification and transplantation combined with chemotherapy for lung cancer in mice.
OBJECTIVE: To investigate the isolation and identification of human umbilical cord mesenchymal stem cells and its transplantation combined with chemotherapy for lung cancer in mice.
METHODS: Human umbilical cord mesenchymal stem cells were isolated from fresh umbilical cord of newborns and identified using tissue culture and enzyme digestion. Twenty Balb/C nude mouse models of lung cancer were randomly divided into two groups: mice in chemotherapy group were given chemotherapy, and those in combined group given combination of chemotherapy with human umbilical cord mesenchymal stem cell transplantation.
RESULTS AND CONCLUSION: Compared with the chemotherapy group, the gastrointestinal tract was rosy and shiny, intestinal mucosa was smooth and complete, and tumor mass and blood indexes significantly decreased in the combined group (P < 0.05). To conclude, mature human umbilical cord mesenchymal stem cells can be obtained by tissue culture and enzyme digestion, and the cell transplantation combined with chemotherapy can significantly reduce gastrointestinal tract damage and the make peripheral hemogram in a stable level.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stem Cells, Lung Neoplasms, Umbilical Cord, Tissue Engineering

CLC Number:

R394.2

Zheng Tian-liang, Zhao Song, Guo Hai-zhou, Cui Guang-hui, Lin Da-wei. Human umbilical cord mesenchymal stem cells: isolation, identification and transplantation combined with chemotherapy for lung cancer in mice[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(23): 3457-3463.

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