Sex differences in the number and activity of circulating endothelial progenitor cells in prehypertension

doi:10.3969/j.issn.2095-4344.2015.19.019

Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (19): 3061-3066.doi: 10.3969/j.issn.2095-4344.2015.19.019

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Sex differences in the number and activity of circulating endothelial progenitor cells in prehypertension

Jiang Yan-ping¹, Zeng Gao-feng¹, Ren Zi², Zeng Hai-tao^{2, 3,} Yang Zhen4

¹Department of Cardiology, the Second Affiliated Hospital of University of South China, Hengyang 421001, Hunan Province, China; ²Center for Reproductive Medicine, the Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou 510000, Guangdong Province, China; ³Department of Obstetrics and Gynaecology, the Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510000, Guangdong Province, China; 4Department of Cardiology, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China

Online:2015-05-06 Published:2015-05-06
Contact: Yang Zhen, M.D., Associate chief physician, Master’s supervisor, Department of Cardiology, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China Zeng Gao-feng, M.D., Chief physician, Professor, Master’s supervisor, Department of Cardiology, the Second Affiliated Hospital of University of South China, Hengyang 421001, Hunan Province, China
About author:Jiang Yan-ping, Studying for master’s degree, Department of Cardiology, the Second Affiliated Hospital of University of South China, Hengyang 421001, Hunan Province, China
Supported by:
the National Natural Science Foundation of China, No. 31270992, 30800215; the Natural Science Foundation of Guangdong Province, No. 2014A030313086; the Science and Technology Plan of Guangdong Province, No. 2013B021800275; Zhujiang Star Project of Guangzhou Science and Technology Plan, No. 2013J2200019; the Fundamental Research Fund for Young Teacher Culture in Sun Yat-sen University, No. 13ykpy20

Abstract

Abstract:

BACKGROUND: The sex differences in circulating endothelial progenitor cells under the condition of prehypertension are still unclear.
OBJECTIVE: To investigate whether there is a sex difference in circulating endothelial progenitor cells in prehypertension.
METHODS: Seventy-nine volunteers, (46.4±4.5) years old, were divided into four groups: normotensive
premenopausal women (n=18), prehypertensive premenopausal women (n=21), normotensive men (n=21) and prehypertensive men (n=19). Flow cytometry analysis was used to evaluate the number of CD34 and KDR double-positive labeled circulating endothelial progenitor cells in the four groups, and acetylated low density lipoprotein (LDL) and lectin fluorescent staining method were used to evaluate the number of cultured endothelial progenitor cells. The migration and proliferation of endothelial progenitor cells were detected. In addition, the estradiol level in plasma was measured in the four groups.
RESULTS AND CONCLUSION: Flow cytometry analysis showed that the number of CD34+/KDR+ circulating endothelial progenitor cells in normotensive and prehypertensive premenopausal women was higher than that in normotensive and prehypertensive men (all P < 0.05). The acetylated-LDL and lectin fluorescent staining method indicated that the cultured endothelial progenitor cells also increased in normotensive and prehypertensive premenopausal women (P < 0.05). The proliferative and migratory activities of cultured endothelial progenitor cells were higher in normotensive and prehypertensive premenopausal women compared with normotensive and prehypertensive men (P < 0.05). There was no difference in the migratory or proliferative activity of endothelial progenitor cells between normotensive and prehypertensive premenopausal women (P > 0.05). However, the migratory or proliferative activity of endothelial progenitor cells was higher in normotensive men than in prehypertensive men (P < 0.05). The level of plasma estradiol in normotensive women and prehypertensive premenopausal women was significantly higher than that in normotensive and prehypertensive men (P < 0.05). The plasma estradiol level showed a linear correlation with the number or activity of circulating endothelial progenitor cells (P < 0.05)．In all, sex differences in the number or activity of endothelial progenitor cells exist in prehypertension. Therefore, the number and activity of circulating endothelial progenitor cells in prehypertensive premenopausal women were preserved when compared with prehypertensive men, which may be related to the enhanced plasma estradiol level.

Key words: Vascular Diseases, Estrogens, Cell Movement, Cell Proliferation

CLC Number:

R394.2

Figures/Tables 5

References

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Sex differences in the number and activity of circulating endothelial progenitor cells in prehypertension

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